LOCUS       BBa_K1022106            1284 bp    DNA     linear       17-May-2024
DEFINITION  pT7:  RBS: His6- SUMO: Magainin II: RBS: TetR:TT
ACCESSION   BBa_K1022106
VERSION     BBa_K1022106.1  
FEATURES             Location/Qualifiers
     promoter        1..28
                     /label=T7
     RBS             29..42
                     /label=34
     misc_feature    43..363
                     /label=His6:SUMO
     misc_feature    364..436
                     /label=Magainin II
     misc_feature    1..436
                     /label=BBa_K1022103
     misc_feature    449..452
                     /label=conserved
     RBS             445..456
                     /label=BBa_B0034
     CDS             466..1082
                     /label=tetR
     misc_feature    1083..1116
                     /label=SsrA
     misc_feature    1123..1147
                     /label=Help:Barcodes
     CDS             463..1122
                     /label=BBa_C0040
     stem_loop       1167..1210
                     /label=stem_loop
     misc_feature    1156..1235
                     /label=BBa_B0010
     terminator      1156..1235
                     /label=BBa_B0010
     stem_loop       1251..1270
                     /label=T7 TE
     misc_feature    1277..1277
                     /label=stop
     polyA_site      1271..1284
                     /label=polya
     misc_feature    1244..1284
                     /label=BBa_B0012
     terminator      1244..1284
                     /label=BBa_B0012
     misc_feature    445..1284
                     /label=BBa_P0440
ORIGIN
        1 taatacgact cactataggg agagaaagag gagaaatact agatggctag catgcatcat
       61 catcatcatc attcggactc agaagtcaat caagaagcta agccagaggt caagccagaa
      121 gtcaagcctg agactcacat caatttaaag gtgtccgatg gatcttcaga gatcttcttc
      181 aagatcaaaa agaccactcc tttaagaagg ctgatggaag cgttcgctaa acgtcagggt
      241 aaggaaatgg actccttaag attcttgtac gacggtatta ggattcaagc tgatcagacc
      301 cctgaagatt tggacatgga tgataacgat attattgagg ctcacagaga acagaccggt
      361 ggtggtatcg gtaaattcct gcactctgcg aaaaaattcg gtaaagcgtt cgttggtcag
      421 atcatgaact cttaattact agagaaagag gagaaatact agatgtccag attagataaa
      481 agtaaagtga ttaacagcgc attagagctg cttaatgagg tcggaatcga aggtttaaca
      541 acccgtaaac tcgcccagaa gctaggtgta gagcagccta cattgtattg gcatgtaaaa
      601 aataagcggg ctttgctcga cgccttagcc attgagatgt tagataggca ccatactcac
      661 ttttgccctt tagaagggga aagctggcaa gattttttac gtaataacgc taaaagtttt
      721 agatgtgctt tactaagtca tcgcgatgga gcaaaagtac atttaggtac acggcctaca
      781 gaaaaacagt atgaaactct cgaaaatcaa ttagcctttt tatgccaaca aggtttttca
      841 ctagagaatg cattatatgc actcagcgct gtggggcatt ttactttagg ttgcgtattg
      901 gaagatcaag agcatcaagt cgctaaagaa gaaagggaaa cacctactac tgatagtatg
      961 ccgccattat tacgacaagc tatcgaatta tttgatcacc aaggtgcaga gccagccttc
     1021 ttattcggcc ttgaattgat catatgcgga ttagaaaaac aacttaaatg tgaaagtggg
     1081 tccgctgcaa acgacgaaaa ctacgcttta gtagcttaat aacactgata gtgctagtgt
     1141 agatcactac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
     1201 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt
     1261 cgggtgggcc tttctgcgtt tata
//