LOCUS       BBa_K1022114            1839 bp    DNA     linear       01-Jun-2024
DEFINITION  pT7 : Lysis Device
ACCESSION   BBa_K1022114
VERSION     BBa_K1022114.1  
FEATURES             Location/Qualifiers
     promoter        1..46
                     /label=BBa_I712074
     CDS             71..727
                     /label=T4 holin
     CDS             55..730
                     /label=BBa_K112805
     CDS             755..1249
                     /label=T4 Endolysin
     CDS             739..1252
                     /label=BBa_K112806
     stem_loop       1272..1315
                     /label=stem_loop
     misc_feature    1261..1340
                     /label=BBa_B0010
     terminator      1261..1340
                     /label=BBa_B0010
     stem_loop       1356..1375
                     /label=T7 TE
     misc_feature    1382..1382
                     /label=stop
     polyA_site      1376..1389
                     /label=polya
     misc_feature    1349..1389
                     /label=BBa_B0012
     terminator      1349..1389
                     /label=BBa_B0012
     promoter        1398..1432
                     /label=BBa_J23116
     CDS             1455..1748
                     /label=T4 antiholin
     CDS             1441..1751
                     /label=BBa_K112807
     stem_loop       1771..1814
                     /label=stem_loop
     misc_feature    1760..1839
                     /label=BBa_B0010
     terminator      1760..1839
                     /label=BBa_B0010
     misc_feature    55..1839
                     /label=BBa_K112808
ORIGIN
        1 taatacgact cactataggg aatacaagct acttgttctt tttgcatact agagcttaaa
       61 aggagggtct atggcagcac ctagaatatc attttcgccc tctgatattc tatttggtgt
      121 tctcgatcgc ttgttcaaag ataacgctac cgggaaggtt cttgcttccc gggtagctgt
      181 cgtaattctt ttgtttataa tggcgattgt ttggtatagg ggagatagtt tctttgagta
      241 ctataagcaa tcaaagtatg aaacatacag tgaaattatt gaaaaggaaa gaactgcacg
      301 ctttgaatct gtcgccctgg aacaactcca gatagttcat atatcatctg aggcagactt
      361 tagtgcggtg tattctttcc gccctaaaaa cttaaactat tttgttgata ttatagcata
      421 cgaaggaaaa ttaccttcaa caataagtga aaaatcactt ggaggatatc ctgttgataa
      481 aactatggat gaatatacag ttcatttaaa tggacgtcat tattattcca actcaaaatt
      541 tgctttttta ccaactaaaa agcctactcc cgaaataaac tacatgtaca gttgtccata
      601 ttttaatttg gataatatct atgctggaac gataaccatg tactggtata gaaatgatca
      661 tataagtaat gaccgccttg aatcaatatg tgctcaggcg gccagaatat taggaagggc
      721 taaataatta tactagagat acttaggagg tattatgaat atatttgaaa tgttacgtat
      781 agatgaaggt cttagactta aaatctataa agacacagaa ggctattaca ctattggcat
      841 cggtcatttg cttacaaaaa gtccatcact taatgctgct aaatctgaat tagataaagc
      901 tattgggcgt aattgcaatg gtgtaattac aaaagatgag gctgaaaaac tctttaatca
      961 ggatgttgat gctgctgttc gcggaatcct gagaaatgct aaattaaaac cggtttatga
     1021 ttctcttgat gcggttcgtc gctgtgcatt gattaatatg gttttccaaa tgggagaaac
     1081 cggtgtggca ggatttacta actctttacg tatgcttcaa caaaaacgct gggatgaagc
     1141 agcagttaac ttagctaaaa gtagatggta taatcaaaca cctaatcgcg caaaacgagt
     1201 cattacaacg tttagaactg gcacttggga cgcgtataaa aatctataaa gctactagag
     1261 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
     1321 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
     1381 gcgtttatat actagagttg acagctagct cagtcctagg gactatgcta gctactagag
     1441 gataggaggc ctttatggcc ttaaaagcaa cagcactttt tgccatgcta ggattgtcat
     1501 ttgttttatc tccatcgatt gaagcgaatg tcgatcctca ttttgataaa tttatggaat
     1561 ctggtattag gcacgtttat atgctttttg aaaataaaag cgtagaatcg tctgaacaat
     1621 tctatagttt tatgagaacg acctataaaa atgacccgtg ctcttctgat tttgaatgta
     1681 tagagcgagg cgcggagatg gcacaatcat acgctagaat tatgaacatt aaattggaga
     1741 ctgaatgaaa ttactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg
     1801 gcctttcgtt ttatctgttg tttgtcggtg aacgctctc
//