LOCUS BBa_K1022127 7154 bp DNA linear 01-Jun-2024 DEFINITION pBAD: Agr A : Agr C : pP2 : RBS: His6- SUMO: MaximinH5: RBS TetR :TT : pTet: cI: TT : pcI: Ulp : Lys ACCESSION BBa_K1022127 VERSION BBa_K1022127.1 FEATURES Location/Qualifiers misc_feature 1..2299 /label=BBa_K1022119 misc_feature 1..130 /label=BBa_K206000 promoter 1..131 /label=promoter misc_feature 40..57 /label=AraI1 misc_feature 61..78 /label=AraI2 misc_feature 139..2058 /label=BBa_I746100 RBS 139..150 /label=B0034-sequence RBS misc_feature 139..2299 /label=BBa_I746103 CDS 157..1404 /label=agrC coding sequence misc_feature 1405..1410 /label=BamHI site RBS 1411..1422 /label=B0034-sequence RBS CDS 1429..2058 /label=agrA coding sequence misc_feature 2067..2146 /label=BBa_B0010 stem_loop 2078..2121 /label=stem_loop misc_feature 2155..2195 /label=BBa_B0012 stem_loop 2162..2181 /label=T7 TE polyA_site 2182..2195 /label=polya misc_feature 2188..2188 /label=stop misc_feature 2204..2299 /label=BBa_I746104 RBS 2300..2313 /label=34 misc_feature 2314..2634 /label=HIs 6 - SUMO misc_feature 2635..2698 /label=Maximin H5 RBS 2703..2706 /label=34 CDS 2720..3335 /label=TetR misc_feature 3410..3489 /label=B0010 misc_feature 3498..3538 /label=B0012 misc_feature 1..3538 /label=BBa_K1022120 misc_feature 1..3538 /label=BBa_K1022120 stem_loop 2078..2121 /label=stem_loop misc_feature 2067..2146 /label=BBa_B0010 terminator 2067..2146 /label=BBa_B0010 stem_loop 2162..2181 /label=T7 TE misc_feature 2188..2188 /label=stop polyA_site 2182..2195 /label=polya misc_feature 2155..2195 /label=BBa_B0012 terminator 2155..2195 /label=BBa_B0012 misc_feature 3547..3565 /label=TetR 1 misc_feature 3547..3600 /label=BBa_R0040 promoter 3566..3571 /label=-35 misc_feature 3572..3590 /label=TetR 2 promoter 3589..3594 /label=-10 promoter 3547..3600 /label=BBa_R0040 misc_feature 3613..3616 /label=conserved RBS 3609..3620 /label=BBa_B0034 promoter 3547..3620 /label=BBa_J13002 CDS 3630..4337 /label=cI lambda misc_feature 4338..4370 /label=LVA misc_feature 4377..4401 /label=Help:Barcodes CDS 3627..4401 /label=BBa_C0051 stem_loop 4421..4464 /label=stem_loop misc_feature 4410..4489 /label=BBa_B0010 terminator 4410..4489 /label=BBa_B0010 stem_loop 4505..4524 /label=T7 TE misc_feature 4531..4531 /label=stop polyA_site 4525..4538 /label=polya misc_feature 4498..4538 /label=BBa_B0012 terminator 4498..4538 /label=BBa_B0012 terminator 4410..4538 /label=BBa_B0015 misc_feature 3547..4538 /label=BBa_K1022108 promoter 4547..4595 /label=cI RBS 4604..4620 /label=34 misc_feature 4621..5361 /label=Ulp misc_feature 4547..5361 /label=BBa_K1022107 misc_feature 3547..5361 /label=BBa_K1022115 misc_feature 5370..7154 /label=BBa_K1022109 misc_feature 3547..7154 /label=BBa_K1022123 ORIGIN 1 acattgatta tttgcacggc gtcacacttt gctatgccat agcaagatag tccataagat 61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt 121 ttgggctagc tactagagaa agaggagaaa tactagatga ttctgatgtt caccattcca 181 gcaattatta gcggtattaa gtacagcaag ctggattatt tcttcatcat cgtaatctct 241 accctgtccc tgttcctgtt caaaatgttc gattccgcga gcctgattat cctgacttcc 301 ttcatcatca tcatgtattt cgtaaaaatc aaatggtata gcatcctgct gatcatgacc 361 tcccagatta tcctgtactg tgccaactac atgtatatcg ttatctacgc gtacatcacg 421 aaaatctccg attctatctt cgttatcttt ccgtctttct tcgtggtgta cgttacgatc 481 tctattctgt tttcctatat tatcaatcgc gttctgaaaa agatctctac tccgtacctg 541 attctgaaca aaggttttct gatcgtcatc tccactatcc tgctgctgac cttctctctg 601 ttcttctttt actctcagat caactccgat gaggcaaaag tcatccgtca gtacagcttc 661 atctttatcg gtattactat cttcctgagc attctgactt tcgttatcag ccagttcctg 721 ctgaaagaaa tgaagtacaa gcgcaaccaa gaagaaatcg agacttacta cgagtacacc 781 ctgaagattg aagcgatcaa caacgaaatg cgtaaatttc gtcacgacta cgttaacatt 841 ctgaccaccc tgagcgaata catccgtgaa gacgacatgc ctggcctgcg tgattatttc 901 aacaaaaaca ttgtgccgat gaaagataac ctgcaaatga acgctattaa gctgaacggt 961 atcgaaaacc tgaaagtacg tgagatcaag ggtctgatta ccgctaaaat cctgcgtgcg 1021 caggaaatga acatcccaat tagcatcgaa atcccagacg aagtttctag cattaacctg 1081 aacatgattg acctgtctcg tagcatcggc atcatcctgg acaacgctat cgaagcttcc 1141 actgaaatcg acgacccgat tattcgcgtt gcttttatcg agagcgaaaa ctccgtgacc 1201 tttatcgtga tgaacaaatg cgcggacgat atcccgcgta ttcacgaact gttccaggaa 1261 tctttcagca ccaagggtga aggtcgcggc ctgggcctgt ccaccctgaa agagatcgcg 1321 gataacgccg acaacgttct gctggacact attatcgaaa acggtttctt cattcagaaa 1381 gtcgaaatca tcaataacta ataaggatcc aaagaggaga aatactagat ggaaatcgca 1441 ctggctacgg acaatccata tgaggttctg gaacaggcta aaaacatgaa cgacatcggc 1501 tgctatttcc tggatattca gctgagcacc gacatcaacg gtatcaaact gggctctgaa 1561 atccgtaaac atgatccggt tggtaacatt attttcgtga cttctcattc tgagctgacc 1621 tatctgacgt tcgtatacaa ggttgcagcc atggatttca tctttaaaga cgatccggca 1681 gagctgcgta cgcgcatcat cgactgtctg gaaactgcgc acacgcgcct gcaactgctg 1741 tccaaagata actccgtgga aaccattgaa ctgaaacgtg gcagcaacag cgtttacgtt 1801 cagtacgatg atatcatgtt cttcgaaagc tccactaagt cccaccgtct gatcgcgcat 1861 ctggataatc gtcagatcga gttctatggc aacctgaaag agctgagcca gctggacgat 1921 cgcttcttcc gttgtcataa ctccttcgtt gttaaccgtc acaacatcga atccatcgac 1981 tccaaagagc gtattgtata cttcaagaac aaagaacact gttatgcgtc tgtacgcaat 2041 gtcaagaaaa tctaataata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa 2101 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac 2161 tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagattaaat acaaattaca 2221 tttaacagtt aagtatttat ttcctacagt taggcaatat aatgataaaa gattgtacta 2281 aatcgtataa tgacagtgaa ggagaaatac tagatggcta gcatgcatca tcatcatcat 2341 cattcggact cagaagtcaa tcaagaagct aagccagagg tcaagccaga agtcaagcct 2401 gagactcaca tcaatttaaa ggtgtccgat ggatcttcag agatcttctt caagatcaaa 2461 aagaccactc ctttaagaag gctgatggaa gcgttcgcta aacgtcaggg taaggaaatg 2521 gactccttaa gattcttgta cgacggtatt aggattcaag ctgatcagac ccctgaagat 2581 ttggacatgg atgataacga tattattgag gctcacagag aacagaccgg tggtatcctg 2641 ggtccggttc tgggtctggt ttctgacacc ctggacgacg ttctgggtat cctgtaataa 2701 agaggagaaa tactagatgt ccagattaga taaaagtaaa gtgattaaca gcgcattaga 2761 gctgcttaat gaggtcggaa tcgaaggttt aacaacccgt aaactcgccc agaagctagg 2821 tgtagagcag cctacattgt attggcatgt aaaaaataag cgggctttgc tcgacgcctt 2881 agccattgag atgttagata ggcaccatac tcacttttgc cctttagaag gggaaagctg 2941 gcaagatttt ttacgtaata acgctaaaag ttttagatgt gctttactaa gtcatcgcga 3001 tggagcaaaa gtacatttag gtacacggcc tacagaaaaa cagtatgaaa ctctcgaaaa 3061 tcaattagcc tttttatgcc aacaaggttt ttcactagag aatgcattat atgcactcag 3121 cgctgtgggg cattttactt taggttgcgt attggaagat caagagcatc aagtcgctaa 3181 agaagaaagg gaaacaccta ctactgatag tatgccgcca ttattacgac aagctatcga 3241 attatttgat caccaaggtg cagagccagc cttcttattc ggccttgaat tgatcatatg 3301 cggattagaa aaacaactta aatgtgaaag tgggtccgct gcaaacgacg aaaactacgc 3361 tttagtagct taataacact gatagtgcta gtgtagatca ctactagagc caggcatcaa 3421 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg 3481 aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatata 3541 ctagagtccc tatcagtgat agagattgac atccctatca gtgatagaga tactgagcac 3601 tactagagaa agaggagaaa tactagatga gcacaaaaaa gaaaccatta acacaagagc 3661 agcttgagga cgcacgtcgc cttaaagcaa tttatgaaaa aaagaaaaat gaacttggct 3721 tatcccagga atctgtcgca gacaagatgg ggatggggca gtcaggcgtt ggtgctttat 3781 ttaatggcat caatgcatta aatgcttata acgccgcatt gcttgcaaaa attctcaaag 3841 ttagcgttga agaatttagc ccttcaatcg ccagagaaat ctacgagatg tatgaagcgg 3901 ttagtatgca gccgtcactt agaagtgagt atgagtaccc tgttttttct catgttcagg 3961 cagggatgtt ctcacctgag cttagaacct ttaccaaagg tgatgcggag agatgggtaa 4021 gcacaaccaa aaaagccagt gattctgcat tctggcttga ggttgaaggt aattccatga 4081 ccgcaccaac aggctccaag ccaagctttc ctgacggaat gttaattctc gttgaccctg 4141 agcaggctgt tgagccaggt gatttctgca tagccagact tgggggtgat gagtttacct 4201 tcaagaaact gatcagggat agcggtcagg tgtttttaca accactaaac ccacagtacc 4261 caatgatccc atgcaatgag agttgttccg ttgtggggaa agttatcgct agtcagtggc 4321 ctgaagagac gtttggcgct gcaaacgacg aaaactacgc tttagtagct taataacgct 4381 gatagtgcta gtgtagatcg ctactagagc caggcatcaa ataaaacgaa aggctcagtc 4441 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca 4501 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagtaac accgtgcgtg 4561 ttgactattt tacctctggc ggtgataatg gttgcttacc ggaaagagga gaaatactag 4621 atggctagcc ttgttcctga attaaatgaa aaagacgatg accaagtaca aaaagctttg 4681 gcatcgagag aaaatactca gttaatgaat agagataata tagagataac agtacgtgat 4741 tttaagacct tggcaccacg aagatggcta aatgacacta tcattgagtt ttttatgaaa 4801 tacattgaaa aatctacccc taatacagtg gcgtttaatt catttttcta taccaattta 4861 tcagaaaggg gttatcaagg cgtccggagg tggatgaaga gaaagaagac acaaattgat 4921 aaacttgata aaatctttac accaataaat ttgaaccaat cccactgggc gttgggcata 4981 attgatttaa aaaagaaaac tataggttac gtagattcat tatcgaatgg tccaaatgct 5041 atgagtttcg ctatactgac tgacttgcaa aaatatgtta tggaggaaag taagcataca 5101 ataggagaag actttgattt gattcattta gattgtccgc agcaaccaaa tggctacgac 5161 tgtggaatat atgtttgtat gaatactctc tatggaagtg cagatgcgcc attggatttt 5221 gattataaag atgcgattag gatgagaaga tttattgccc atttgatttt aaccgacgct 5281 ttaaaacggg atccgaactc gagctccgtc gacaagcttg cggccgcact cgagcaccac 5341 caccaccacc actgagtaca ttactagagc ttaaaaggag ggtctatggc agcacctaga 5401 atatcatttt cgccctctga tattctattt ggtgttctcg atcgcttgtt caaagataac 5461 gctaccggga aggttcttgc ttcccgggta gctgtcgtaa ttcttttgtt tataatggcg 5521 attgtttggt ataggggaga tagtttcttt gagtactata agcaatcaaa gtatgaaaca 5581 tacagtgaaa ttattgaaaa ggaaagaact gcacgctttg aatctgtcgc cctggaacaa 5641 ctccagatag ttcatatatc atctgaggca gactttagtg cggtgtattc tttccgccct 5701 aaaaacttaa actattttgt tgatattata gcatacgaag gaaaattacc ttcaacaata 5761 agtgaaaaat cacttggagg atatcctgtt gataaaacta tggatgaata tacagttcat 5821 ttaaatggac gtcattatta ttccaactca aaatttgctt ttttaccaac taaaaagcct 5881 actcccgaaa taaactacat gtacagttgt ccatatttta atttggataa tatctatgct 5941 ggaacgataa ccatgtactg gtatagaaat gatcatataa gtaatgaccg ccttgaatca 6001 atatgtgctc aggcggccag aatattagga agggctaaat aattatacta gagatactta 6061 ggaggtatta tgaatatatt tgaaatgtta cgtatagatg aaggtcttag acttaaaatc 6121 tataaagaca cagaaggcta ttacactatt ggcatcggtc atttgcttac aaaaagtcca 6181 tcacttaatg ctgctaaatc tgaattagat aaagctattg ggcgtaattg caatggtgta 6241 attacaaaag atgaggctga aaaactcttt aatcaggatg ttgatgctgc tgttcgcgga 6301 atcctgagaa atgctaaatt aaaaccggtt tatgattctc ttgatgcggt tcgtcgctgt 6361 gcattgatta atatggtttt ccaaatggga gaaaccggtg tggcaggatt tactaactct 6421 ttacgtatgc ttcaacaaaa acgctgggat gaagcagcag ttaacttagc taaaagtaga 6481 tggtataatc aaacacctaa tcgcgcaaaa cgagtcatta caacgtttag aactggcact 6541 tgggacgcgt ataaaaatct ataaagctac tagagccagg catcaaataa aacgaaaggc 6601 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta 6661 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tatatactag agttgacagc 6721 tagctcagtc ctagggacta tgctagctac tagaggatag gaggccttta tggccttaaa 6781 agcaacagca ctttttgcca tgctaggatt gtcatttgtt ttatctccat cgattgaagc 6841 gaatgtcgat cctcattttg ataaatttat ggaatctggt attaggcacg tttatatgct 6901 ttttgaaaat aaaagcgtag aatcgtctga acaattctat agttttatga gaacgaccta 6961 taaaaatgac ccgtgctctt ctgattttga atgtatagag cgaggcgcgg agatggcaca 7021 atcatacgct agaattatga acattaaatt ggagactgaa tgaaattact agagccaggc 7081 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt 7141 cggtgaacgc tctc //