LOCUS       BBa_K1025007            1821 bp    DNA     linear       12-Jun-2024
DEFINITION  Bitter Defender Part（RBS_B0032）
ACCESSION   BBa_K1025007
VERSION     BBa_K1025007.1  
FEATURES             Location/Qualifiers
     promoter        1..62
                     /label=tac promoter
     RBS             63..72
                     /label=RBS_tac
     CDS             81..155
                     /label=tnaC
     misc_feature    156..362
                     /label=Rho binding site
     RBS             363..375
                     /label=RBS_B0032
     CDS             376..1575
                     /label=tetA
     misc_feature    1664..1821
                     /label=rrnB_ternimator
ORIGIN
        1 ttgacaatta atcatccggc tcgtataatg tgtggaattg tgagcggata acaatttcac
       61 acaggaaaca gaccatggct atgaatatct tacatatatg tgtgacctca aaatggttca
      121 atattgacaa caaaattgtc gatcaccgcc cttgatttgc ccttctgtag ccatcaccag
      181 agccaaaccg attagattca atgtgatcta tttgtttgct atatcttaat tttgcctttt
      241 gcaaaggtca tctctcgttt atttacttgt tttagtaaat gatggtgctt gcatatatat
      301 ctggcgaatt aatcggtata gcagatgtaa tattcacagg gatcactgta attaaaataa
      361 attcacacag gaaagatgaa acccaacata cccctgatcg taattctgag cactgtcgcg
      421 ctcgacgctg tcggcatcgg cctgattatg ccggtgctgc cgggcctcct gcgcgatctg
      481 gttcactcga acgacgtcac cgcccactat ggcattctgc tggcgctgta tgcgttggtg
      541 caatttgcct gcgcacctgt gctgggcgcg ctgtcggatc gtttcgggcg gcggccaatc
      601 ttgctcgtct cgctggccgg cgccactgtc gactacgcca tcatggcgac agcgcctttc
      661 ctttgggttc tctatatcgg gcggatcgtg gccggcatca ccggggcgac tggggcggta
      721 gccggcgctt atattgccga tatcactgat ggcgatgagc gcgcgcggca cttcggcttc
      781 atgagcgcct gtttcgggtt cgggatggtc gcgggacctg tgctcggtgg gctgatgggc
      841 ggtttctccc cccacgctcc gttcttcgcc gcggcagcct tgaacggcct caatttcctg
      901 acgggctgtt tccttttgcc ggagtcgcac aaaggcgaac gccggccgtt acgccgggag
      961 gctctcaacc cgctcgcttc gttccggtgg gcccggggca tgaccgtcgt cgccgccctg
     1021 atggcggtct tcttcatcat gcaacttgtc ggacaggtgc cggccgcgct ttgggtcatt
     1081 ttcggcgagg atcgctttca ctgggacgcg accacgatcg gcatttcgct tgccgcattt
     1141 ggcattctgc attcactcgc ccaggcaatg atcaccggcc ctgtagccgc ccggctcggc
     1201 gaaaggcggg cactcatgct cggaatgatt gccgacggca caggctacat cctgcttgcc
     1261 ttcgcgacac ggggatggat ggcgttcccg atcatggtcc tgcttgcttc gggtggcatc
     1321 ggaatgccgg cgctgcaagc aatgttgtcc aggcaggtgg atgaggaacg tcaggggcag
     1381 ctgcaaggct cactggcggc gctcaccagc ctgacctcga tcgtcggacc cctcctcttc
     1441 acggcgatct atgcggcttc tataacaacg tggaacgggt gggcatggat tgcaggcgct
     1501 gccctctact tgctctgcct gccggcgctg cgtcgcgggc tttggagcgg cgcagggcaa
     1561 cgagccgatc gctgaaagct tggctgtttt ggcggatgag agaagatttt cagcctgata
     1621 cagattaaat cagaacgcag aagcggtctg ataaaacaga atttgcctgg cggcagtagc
     1681 gcggtggtcc cacctgaccc catgccgaac tcagaagtga aacgccgtag cgccgatggt
     1741 agtgtggggt ctccccatgc gagagtaggg aactgccagg catcaaataa aacgaaaggc
     1801 tcagtcgaaa gactgggcct t
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