LOCUS       BBa_K1030004            1980 bp    DNA     linear       14-May-2024
DEFINITION  J23104->tetA-asv+sfGFP
ACCESSION   BBa_K1030004
VERSION     BBa_K1030004.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23104
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             64..1254
                     /label=tetA(C) backwards
     misc_feature    64..66
                     /label=stop
     primer_bind     64..83
                     /label=Rev primer J31006
     primer_bind     1231..1254
                     /label=Fwd primer J31006
     CDS             64..1254
                     /label=BBa_J31006
     misc_feature    1261..1263
                     /label=start
     CDS             1261..1980
                     /label=superfolder GFP coding region
     misc_feature    1975..1980
                     /label=stop
     CDS             1261..1980
                     /label=BBa_I746916
ORIGIN
        1 ttgacagcta gctcagtcct aggtattgtg ctagctacta gagaaagagg agaaatacta
       61 gagttaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag
      121 gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata
      181 aatcgccgtg acgatcagcg gtccaatgat cgaagttagg ctggtaagag ccgcgagcga
      241 tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc
      301 gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg
      361 cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc
      421 ctgcttctcg ccgaaacgtt tggtggcggg gccagtgacg aaggcttgag cgagggcgtg
      481 caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc
      541 ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa
      601 gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg
      661 gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa
      721 gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa
      781 ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca
      841 agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata
      901 ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag
      961 gatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga
     1021 agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca
     1081 tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc
     1141 ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac
     1201 agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcattactag
     1261 atgcgtaaag gcgaagagct gttcactggt gtcgtcccta ttctggtgga actggatggt
     1321 gatgtcaacg gtcataagtt ttccgtgcgt ggcgagggtg aaggtgacgc aactaatggt
     1381 aaactgacgc tgaagttcat ctgtactact ggtaaactgc cggtaccttg gccgactctg
     1441 gtaacgacgc tgacttatgg tgttcagtgc tttgctcgtt atccggacca tatgaagcag
     1501 catgacttct tcaagtccgc catgccggaa ggctatgtgc aggaacgcac gatttccttt
     1561 aaggatgacg gcacgtacaa aacgcgtgcg gaagtgaaat ttgaaggcga taccctggta
     1621 aaccgcattg agctgaaagg cattgacttt aaagaagacg gcaatatcct gggccataag
     1681 ctggaataca attttaacag ccacaatgtt tacatcaccg ccgataaaca aaaaaatggc
     1741 attaaagcga attttaaaat tcgccacaac gtggaggatg gcagcgtgca gctggctgat
     1801 cactaccagc aaaacactcc aatcggtgat ggtcctgttc tgctgccaga caatcactat
     1861 ctgagcacgc aaagcgttct gtctaaagat ccgaacgaga aacgcgatca tatggttctg
     1921 ctggagttcg taaccgcagc gggcatcacg catggtatgg atgaactgta caaatgatga
//