LOCUS       BBa_K103020              501 bp    DNA     linear       01-Jun-2024
DEFINITION  omega_linker under PT7
ACCESSION   BBa_K103020
VERSION     BBa_K103020.1  
FEATURES             Location/Qualifiers
     promoter        12..28
                     /label=T7
     misc_feature    32..57
                     /label=lacO
     RBS             92..96
                     /label=rbs
     misc_feature    116..130
                     /label=HisTag
     misc_feature    143..160
                     /label=thrombin
     CDS             104..166
                     /label=HisTagged ORF
     misc_feature    161..166
                     /label=NdeI
     misc_feature    102..107
                     /label=NcoI
     misc_feature    104..106
                     /label=ATG
     misc_feature    164..166
                     /label=ATG
     promoter        1..166
                     /label=BBa_K103021
     CDS             167..225
                     /label=linker
     CDS             226..501
                     /label=omega
     misc_feature    168..173
                     /label=SacI
     misc_feature    167..501
                     /label=BBa_K103013
ORIGIN
        1 atcccgcgaa attaatacga ctcactatag gggaattgtg agcggataac aattcccctc
       61 tagctagaaa taattttgtt taactttaag aaggagatat accatgggca gcagccatca
      121 tcatcatcat cacagcagcg gcctggtgcc gcgcggcagc catatgggag ctctggtgga
      181 agcggtggcg gaggatctgg aggaggtggt tctggaggtg gaggtctact tactctagct
      241 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
      301 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
      361 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
      421 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
      481 tcactgatta agcattggta a
//