LOCUS       BBa_K1040004             302 bp    DNA     linear       25-May-2024
DEFINITION  toggle switch construction kit ( ptet_rbs_LacI_rbs )
ACCESSION   BBa_K1040004
VERSION     BBa_K1040004.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             83..170
                     /label=end of LacI coding region (inactive)
     misc_feature    83..282
                     /label=BBa_R0010
     misc_feature    171..208
                     /label=CAP binding site
     promoter        219..224
                     /label=-35
     promoter        243..248
                     /label=-10
     misc_feature    248..282
                     /label=LacI binding site
     misc_feature    255..255
                     /label=start
     promoter        83..282
                     /label=BBa_R0010
     misc_feature    295..298
                     /label=conserved
     RBS             291..302
                     /label=BBa_B0034
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag agcaatacgc aaaccgcctc tccccgcgcg ttggccgatt
      121 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
      181 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
      241 cgtatgttgt gtggaattgt gagcggataa caatttcaca catactagag aaagaggaga
      301 aa
//