BBa_K1051367 1 BBa_K1051367 K1051302 (clb5 promoter) + K1051003 (non stop codon GFP) + K1051006 (Stop codon + TBY-1 terminator) 2013-07-18T11:00:00Z 2015-05-08T01:08:54Z 1332bp clb5 promoter+GFP+stop codon false false _1358_ 0 15940 9 It's complicated false biobrick false Kequan Lin component2331822 1 BBa_K1051003 component2331821 1 BBa_K1051302 component2331823 1 BBa_K1051006 annotation2331823 1 BBa_K1051006 range2331823 1 1377 1407 annotation2331821 1 BBa_K1051302 range2331821 1 1 648 annotation2331822 1 BBa_K1051003 range2331822 1 655 1368 BBa_K1051302 1 BBa_K1051302 clb5 promoter (during G1 phase) 2013-06-29T11:00:00Z 2015-05-08T01:08:54Z CGATCCTGAGTGCGAGCTTTCTTTTTTAGACTACTAATTTTATTTTGCTAGTCATTTTTT TTTTATACTCAAAAAGTAAAAAGACTACGAGTATATTCAAAGTAAAAAACGAACGTCAAA CTATCTCGATTAAAACTTGTCATACTGTGGGTATCATATTCTGTGCCCTCAGTGAAAAGA ACCAGCAAAAGAACGCGCATCTCGAGTGAAGACGCGCCCTTGATGGTACAAAATTTAACG GGAAGGCGCGTCGTGATGTTCACGCGCTTTGCCCACATTGGGATAGCGCCCACAGCATAT CTGTGCTAAACTCACTTTTCCTAGTGACTGCCGATAGCTACTGCCATCTACCGCGAAGGG AACTTCATTTGCGTTCATCGGTTTATTAGAAGCTACTTGGAACTAATTCTTAAGCTTCTC AAGAAAAGTTTTTTTTCTGTCTATCTATTGAAGTCTTTTTGTCTTTGTACTTCAAGAGAC TCAATCACCTAAAGCTTTTCACGGCCAATTAGTTGTCTCACACAAAGCAAAATAAGCTTA ATAATTAGCAGTAACGCGCTTTTCCCTGTATTTAAAGCCGCTGAACACCTTTACTGAACA It is a promoter which promotes transciption in G1/S of the yeast cell cycle .If you want to express your proteins in G1/S of the yeast cell cycle ,you can add the sequence which is actived by clb5 before your gene sequence ,then put them in a yeast plasmid .After transformation ,you can get the protein you want from the metabolic product of the Bacterial you use for transformation . false false _1358_ 0 15940 9 Not in stock false We get the sequence which is actived by clb5 from a yeast database (http://www.ncbi.nlm.nih.gov/CBBresearch/Landsman/Cell_cycle_data/) .You can amplify it from the yeast gene using PCR .See the primers as follows : upstream:GTTTCTTCGAATTCGCGGCCGCTTCTAGAGCGATCCTGAGTGCGAGCTTT downstream:GTTTCTTCCTGCAGCGGCCGCTACTAGTATGTTCAGTAAAGGTGTTCAG false Kequan Lin BBa_K1051003 1 BBa_K1051003 Stop codon free GFP in RFC[23] standard 2013-07-03T11:00:00Z 2015-05-08T01:08:53Z kit GFP without stop coding false false _1358_ 0 15912 9 In stock false add prefix suffix del stop coding false Xiang LI BBa_K1051006 1 BBa_K1051006 Saccharomycescerevisiae TBY-1 terminator with stopcodon 2013-07-07T11:00:00Z 2015-05-08T01:08:53Z Saccharomycescerevisiae Saccharomycescerevisiae terminator with stopcodon false false _1358_ 0 15912 9 It's complicated false stopcodon + terminator false Xiang LI BBa_K1051006_sequence 1 taatagtgtatgtgtataatatcatagttta BBa_K1051367_sequence 1 atggcgcagtggtagcgcagcagattgcaaatctgttggtccttagttcgatcctgagtgcgagctttcttttttagactactaattttattttgctagtcattttttttttatactcaaaaagtaaaaagactacgagtatattcaaagtaaaaaacgaacgtcaaactatctcgattaaaacttgtcatactgtgggtatcatattctgtgccctcagtgaaaagaaccagcaaaagaacgcgcatctcgagtgaagacgcgcccttgatggtacaaaatttaacgggaaggcgcgtcgtgatgttcacgcgctttgcccacattgggatagcgcccacagcatatctgtgctaaactcacttttcctagtgactgccgatagctactgccatctaccgcgaagggaacttcatttgcgttcatcggtttattagaagctacttggaactaattcttaagcttctcaagaaaagttttttttctgtctatctattgaagtctttttgtctttgtacttcaagagactcaatcacctaaagcttttcacggccaattagttgtctcacacaaagcaaaataagcttaataattagcagtaacgcgcttttccctgtatttaaagccgctgaacacctttactgaacatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaatactagagtaatagtgtatgtgtataatatcatagttta BBa_K1051003_sequence 1 atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaa BBa_K1051302_sequence 1 atggcgcagtggtagcgcagcagattgcaaatctgttggtccttagttcgatcctgagtgcgagctttcttttttagactactaattttattttgctagtcattttttttttatactcaaaaagtaaaaagactacgagtatattcaaagtaaaaaacgaacgtcaaactatctcgattaaaacttgtcatactgtgggtatcatattctgtgccctcagtgaaaagaaccagcaaaagaacgcgcatctcgagtgaagacgcgcccttgatggtacaaaatttaacgggaaggcgcgtcgtgatgttcacgcgctttgcccacattgggatagcgcccacagcatatctgtgctaaactcacttttcctagtgactgccgatagctactgccatctaccgcgaagggaacttcatttgcgttcatcggtttattagaagctacttggaactaattcttaagcttctcaagaaaagttttttttctgtctatctattgaagtctttttgtctttgtacttcaagagactcaatcacctaaagcttttcacggccaattagttgtctcacacaaagcaaaataagcttaataattagcagtaacgcgcttttccctgtatttaaagccgctgaacacctttactgaaca igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z