BBa_E1010 1 mRFP1 **highly** engineered mutant of red fluorescent protein from Discosoma striata (coral) 2004-07-27T11:00:00Z 2015-08-31T04:07:26Z Campbell et al., PNAS v99 p7877 <a href="http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=12060735">URL</a> Released HQ 2013 monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm false false _11_1_ 0 52 7 In stock false TAATAA double stop codon added (DE). Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A. true Drew Endy annotation2214014 1 Help:Barcodes range2214014 1 682 706 annotation1014044 1 mrfp1 range1014044 1 1 675 BBa_K1055007 1 BBa_K1055007 FsC: functional cutinase 2013-09-17T11:00:00Z 2015-05-08T01:08:57Z fungus Fusarium solani pisi The part enables the user to produce functional cutinase from fungus Fusarium solani pisi which is a cuticula degrading hydrolase. The enzyme is fused to mRFP to allow tracking of expression and also provides a His-Tag for subsequent purification. Both, mRFP and His-Tag, can be cleaved using TEV-Protease. false false _1362_ 0 16980 9 It's complicated false Despite BioBricks the part also consists of the RBS from pET22b(+), a TEV-site and a His-Tag. false Sven Jager, Sebastian Wagner and Anne Schieferdecker component2352868 1 BBa_E1010 component2352865 1 BBa_K808025 component2352862 1 BBa_K206000 component2352864 1 BBa_J32015 annotation2352862 1 BBa_K206000 range2352862 1 1 130 annotation2352868 1 BBa_E1010 range2352868 1 866 1571 annotation2352864 1 BBa_J32015 range2352864 1 137 202 annotation2352865 1 BBa_K808025 range2352865 1 209 859 BBa_K808025 1 BBa_K808025 FsC: Cutinase PET cleaving enzyme 2012-09-01T11:00:00Z 2015-05-08T01:13:26Z Cloned from vector pEST100 from Becker et all 2005: (Becker, S., Theile, S., Heppeler, N., Michalczyk, A., Wentzel, A., Wilhelm, S., Jaeger, K.-E., et al. (2005). A generic system for the Escherichia coli cell-surface display of lipolytic enzymes. FEBS letters, 579(5), 1177???82. doi:10.1016/j.febslet.2004.12.087) Released HQ 2013 A cutinase is a cuticula degrading hydrolase from the fungus Fusarium solani pisi. It shows activity towards PET. false false _1065_ 0 11792 9 In stock false Surface Expression is very hard to do. FsC shows lipolytic activity and attacks the outer membrane of gramm negativ bacteria. This activity leads to a certain toxicity for expression hosts. In addition FsC needs to be expressed in the periplasm due to folding issues. false Marie Burghard, Henrik Cordes, Jascha Diemer, Adrian Eilingsfeld, Sven Jager, Rene Sahm, Arne Wehling BBa_K206000 1 pBAD pBAD strong 2009-10-13T11:00:00Z 2015-05-08T01:11:23Z The sequence was obtained by applying all mutations that upregulated AraC binding and subsequent promoter activity listed in reference [1]. Released HQ 2013 pBAD is an <i>E.coli</i> promoter that is induced by L-arabinose. K206000 is a mutagenized pBAD promoter that is responsive to lower concentrations of arabinose than wild type (<partinfo>I13453</partinfo>) and, additionally, exhibits a higher maximum of protein expression as measured by coupling to a fluorescent reporter. false false _307_ 0 4172 9 In stock true There were no special design considerations. false Amelia Hardjasa annotation2049254 1 AraI2 range2049254 1 61 78 annotation2049252 1 promoter range2049252 1 1 131 annotation2049253 1 AraI1 range2049253 1 40 57 BBa_J32015 1 PelB PelB leader sequence; directs protein to E. coli periplasmic membrane 2006-08-24T11:00:00Z 2015-08-31T04:08:46Z Novagen Released HQ 2013 The pelB leader sequence is a sequence of amino acids which when attached to a protein, directs the protein to the periplasmic membrane of E. coli, where the sequence is removed by pelB peptidase. It is used to direct coat protein-antigen fusions to the cell surface. false true _50_ 0 495 50 In stock true . true Austen Heinz annotation1898182 1 PelB range1898182 1 1 66 BBa_K1055007_sequence 1 acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagatgaaatacctgctgccgaccgctgctgctggtctgctgctcctcgctgcccagccggcgatggcctactagatgctgcctacttctaaccctgctcaggagcttgaggcgcgccagcttggtagaacaactcgcgacgatctgatcaacggcaatagcgcttcctgcgccgatgtcatcttcatttatgcccggggttcaacagagacgggcaacttgggaactctcggtcctagcattgcctccaaccttgagtccgccttcggcaaggacggtgtctggattcagggcgttggcggtgcctaccgagccactcttggagacaatgctctccctcgcggtacctctagcgccgcaatcagggagatgcttggtctcttccagcaggccaacaccaagtgccctgacgcgactttgatcgccggtggctacagccagggtgctgcacttgcggccgcctccatcgaggacctcgactcggccattcgtgacaagatcgccggaactgttctgttcggctacaccaagaacctacagaaccgtggccgaatccccaactaccctgccgacaggaccaaggtcttctgcaatacaggagatctcgtttgtactggtagcttgatcgttgctgcacctcacttggcttatggtcctgatgctcggggccctgcccctgagttcctcatcgagaaggttcgggctgtccgtggttctgcttaataatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc BBa_E1010_sequence 1 atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc BBa_J32015_sequence 1 atgaaatacctgctgccgaccgctgctgctggtctgctgctcctcgctgcccagccggcgatggcc BBa_K206000_sequence 1 acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagc BBa_K808025_sequence 1 atgctgcctacttctaaccctgctcaggagcttgaggcgcgccagcttggtagaacaactcgcgacgatctgatcaacggcaatagcgcttcctgcgccgatgtcatcttcatttatgcccggggttcaacagagacgggcaacttgggaactctcggtcctagcattgcctccaaccttgagtccgccttcggcaaggacggtgtctggattcagggcgttggcggtgcctaccgagccactcttggagacaatgctctccctcgcggtacctctagcgccgcaatcagggagatgcttggtctcttccagcaggccaacaccaagtgccctgacgcgactttgatcgccggtggctacagccagggtgctgcacttgcggccgcctccatcgaggacctcgactcggccattcgtgacaagatcgccggaactgttctgttcggctacaccaagaacctacagaaccgtggccgaatccccaactaccctgccgacaggaccaaggtcttctgcaatacaggagatctcgtttgtactggtagcttgatcgttgctgcacctcacttggcttatggtcctgatgctcggggccctgcccctgagttcctcatcgagaaggttcgggctgtccgtggttctgcttaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z