Adh1P Acce

BBa_K106006 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K106006
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Sergio Peisajovich
Date created: 2008-10-22 11:00:00
Date modified: 2015-05-08 01:08:58

AarI AD acceptor vector (pRS315, Adh1P, Adh1t)



Types
DnaRegion

Roles
Plasmid_Backbone

plasmid_vector

Sequences BBa_K106006_sequence (Version 1)

Description

This is an acceptor vector for AarI cloning, with A and D ends. Cloning into this plasmid will put your parts under the control of a Adh1 promoter (Adh1P) and Adh1 terminator (Adh1t). For more information regarding AarI acceptor vectors, and AarI cloning in general, please visit the UCSF 2008 wiki.

Notes

The pRS315 vector has been modified with an AarI cloning cassette inserted between the PspOMI and SacI sites.

Source

A modified pRS315 vector.

Sequence Annotation Location Component / Role(s)
Adh1 Promoter
Adh1 terminator
AarI AD MCS
CEN ARS
LEU2 marker
ampicillin resistance marker
Stops in all 3 frames
ATG start codon
T7 promoter
lacZalpha reporter
f1 origin
ampicillin resistance promoter
pBR322 origin
lac promoter
T3 promoter
A overhang
D overhang
6132,7646
22,215
4309,4385
3403,3921
1570,2676
4053,4911
11,21
7638,7640
224,242
246,405
427,733
3983,4011
5068,5687
5996,6025
6095,6114
7647,7650
1,4
feature/promoter promoter
feature/stem_loop stem_loop
sequence_feature feature/misc
sequence_feature feature/misc
CDS feature/cds
CDS feature/cds
stop_codon feature/stop
start_codon feature/start
feature/promoter promoter
feature/cds CDS
sequence_feature feature/dna
feature/promoter promoter
sequence_feature feature/dna
feature/promoter promoter
promoter feature/promoter
sequence_feature feature/dna
sequence_feature feature/dna
igem#experience
Works
 
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K106006/1