LOCUS BBa_K106006 7650 bp DNA linear 29-Apr-2024 DEFINITION AarI AD acceptor vector (pRS315, Adh1P, Adh1t) ACCESSION BBa_K106006 VERSION BBa_K106006.1 FEATURES Location/Qualifiers promoter 6132..7646 /label=Adh1 Promoter stem_loop 22..215 /label=Adh1 terminator misc_feature 4309..4385 /label=AarI AD MCS misc_feature 3403..3921 /label=CEN ARS CDS 1570..2676 /label=LEU2 marker CDS 4053..4911 /label=ampicillin resistance marker misc_feature 11..21 /label=Stops in all 3 frames misc_feature 7638..7640 /label=ATG start codon promoter 224..242 /label=T7 promoter CDS 246..405 /label=lacZalpha reporter misc_feature 427..733 /label=f1 origin promoter 3983..4011 /label=ampicillin resistance promoter misc_feature 5068..5687 /label=pBR322 origin promoter 5996..6025 /label=lac promoter promoter 6095..6114 /label=T3 promoter misc_feature 7647..7650 /label=A overhang misc_feature 1..4 /label=D overhang ORIGIN 1 tgcgggatcc tgagtaaata agcggccgcg cgaatttctt atgatttatg atttttatta 61 ttaaataagt tataaaaaaa ataagtgtat acaaatttta aagtgactct taggttttaa 121 aacgaaaatt cttattcttg agtaactctt tcctgtaggt caggttgctt tctcaggtat 181 agcatgaggt cgctcttatt gaccacaccg agctccaatt cgccctatag tgagtcgtat 241 tacaattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa 301 cttaatcgcc ttgcagcaca tccccccttc gccagctggc gtaatagcga agaggcccgc 361 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgcga cgcgccctgt 421 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc 481 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc 541 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg 601 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga 661 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc 721 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg 781 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt 841 aacaaaatat taacgtttac aatttcctga tgcggtattt tctccttacg catctgtgcg 901 gtatttcaca ccgcatatcg accctcgagg agaacttcta gtatatccac atacctaata 961 ttattgcctt attaaaaatg gaatcccaac aattacatca aaatccacat tctcttcaaa 1021 atcaattgtc ctgtacttcc ttgttcatgt gtgttcaaaa acgttatatt tataggataa 1081 ttatactcta tttctcaaca agtaattggt tgtttggccg agcggtctaa ggcgcctgat 1141 tcaagaaata tcttgaccgc agttaactgt gggaatactc aggtatcgta agatgcaaga 1201 gttcgaatct cttagcaacc attatttttt tcctcaacat aacgagaaca cacaggggcg 1261 ctatcgcaca gaatcaaatt cgatgactgg aaattttttg ttaatttcag aggtcgcctg 1321 acgcatatac ctttttcaac tgaaaaattg ggagaaaaag gaaaggtgag aggccggaac 1381 cggcttttca tatagaatag agaagcgttc atgactaaat gcttgcatca caatacttga 1441 agttgacaat attatttaag gacctattgt tttttccaat aggtggttag caatcgtctt 1501 actttctaac ttttcttacc ttttacattt cagcaatata tatatatatt tcaaggatat 1561 accattctaa tgtctgcccc tatgtctgcc cctaagaaga tcgtcgtttt gccaggtgac 1621 cacgttggtc aagaaatcac agccgaagcc attaaggttc ttaaagctat ttctgatgtt 1681 cgttccaatg tcaagttcga tttcgaaaat catttaattg gtggtgctgc tatcgatgct 1741 acaggtgtcc cacttccaga tgaggcgctg gaagcctcca agaaggttga tgccgttttg 1801 ttaggtgctg tgggtggtcc taaatggggt accggtagtg ttagacctga acaaggttta 1861 ctaaaaatcc gtaaagaact tcaattgtac gccaacttaa gaccatgtaa ctttgcatcc 1921 gactctcttt tagacttatc tccaatcaag ccacaatttg ctaaaggtac tgacttcgtt 1981 gttgtcagag aattagtggg aggtatttac tttggtaaga gaaaggaaga cgatggtgat 2041 ggtgtcgctt gggatagtga acaatacacc gttccagaag tgcaaagaat cacaagaatg 2101 gccgctttca tggccctaca acatgagcca ccattgccta tttggtcctt ggataaagct 2161 aatgttttgg cctcttcaag attatggaga aaaactgtgg aggaaaccat caagaacgaa 2221 ttccctacat tgaaggttca acatcaattg attgattctg ccgccatgat cctagttaag 2281 aacccaaccc acctaaatgg tattataatc accagcaaca tgtttggtga tatcatctcc 2341 gatgaagcct ccgttatccc aggttccttg ggtttgttgc catctgcgtc cttggcctct 2401 ttgccagaca agaacaccgc atttggtttg tacgaaccat gccacggttc tgctccagat 2461 ttgccaaaga ataaggttga ccctatcgcc actatcttgt ctgctgcaat gatgttgaaa 2521 ttgtcattga acttgcctga agaaggtaag gccattgaag atgcagttaa aaaggttttg 2581 gatgcaggta tcagaactgg tgatttaggt ggttccaaca gtaccaccga agtcggtgat 2641 gctgtcgccg aagaagttaa gaaaatcctt gcttaaaaag attctctttt tttatgatat 2701 ttgtacataa actttataaa tgaaattcat aatagaaacg acacgaaatt acaaaatgga 2761 atatgttcat agggtagacg aaactatata cgcaatctac atacatttat caagaaggag 2821 aaaaaggagg atagtaaagg aatacaggta agcaaattga tactaatggc tcaacgtgat 2881 aaggaaaaag aattgcactt taacattaat attgacaagg aggagggcac cacacaaaaa 2941 gttaggtgta acagaaaatc atgaaactac gattcctaat ttgatattgg aggattttct 3001 ctaaaaaaaa aaaaatacaa caaataaaaa acactcaatg acctgaccat ttgatggagt 3061 ttaagtcaat accttcttga accatttccc ataatggtga aagttccctc aagaatttta 3121 ctctgtcaga aacggcctta cgacgtagtc gatatggtgc actctcagta caatctgctc 3181 tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg 3241 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat 3301 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg 3361 cctattttta taggttaatg tcatgataat aatggtttct taggacggat cgcttgcctg 3421 taacttacac gcgcctcgta tcttttaatg atggaataat ttgggaattt actctgtgtt 3481 tatttatttt tatgttttgt atttggattt tagaaagtaa ataaagaagg tagaagagtt 3541 acggaatgaa gaaaaaaaaa taaacaaagg tttaaaaaat ttcaacaaaa agcgtacttt 3601 acatatatat ttattagaca agaaaagcag attaaataga tatacattcg attaacgata 3661 agtaaaatgt aaaatcacag gattttcgtg tgtggtcttc tacacagaca agatgaaaca 3721 attcggcatt aatacctgag agcaggaaga gcaagataaa aggtagtatt tgttggcgat 3781 ccccctagag tcttttacat cttcggaaaa caaaaactat tttttcttta atttcttttt 3841 ttactttcta tttttaattt atatatttat attaaaaaat ttaaattata attattttta 3901 tagcacgtga tgaaaaggac ccaggtggca cttttcgggg aaatgtgcgc ggaaccccta 3961 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat 4021 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc 4081 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga 4141 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca 4201 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt 4261 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg 4321 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc 4381 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata 4441 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt 4501 ttcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag 4561 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca 4621 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg 4681 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg 4741 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag 4801 atggtaagcc ctcccgtatc gtagttatct acacgacggg cagtcaggca actatggatg 4861 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag 4921 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga 4981 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt 5041 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc 5101 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc 5161 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac 5221 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac 5281 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt 5341 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct 5401 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat 5461 acctacagcg tgagcattga gaaagcgcca cgcttcccga agggagaaag gcggacaggt 5521 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca ggggggaacg 5581 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt 5641 gatgctcgtc aggggggccg agcctatgga aaaacgccag caacgcggcc tttttacggt 5701 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg 5761 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg 5821 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc 5881 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg 5941 gcagtgagcg caacgcaatt aatgtgagtt acctcactca ttaggcaccc caggctttac 6001 actttatgct tccggctcct atgttgtgtg gaattgtgag cggataacaa tttcacacag 6061 gaaacagcta tgaccatgat tacgccaagc tcggaattaa ccctcactaa agggaacaaa 6121 agctgggtac cgggccctaa aacaagaaga gggttgacta catcacgatg agggggatcg 6181 aagaaatgat ggtaaatgaa ataggaaatc aaggagcatg aaggcaaaag acaaatataa 6241 gggtcgaacg aaaaataaag tgaaaagtgt tgatatgatg tatttggctt tgcggcgccg 6301 aaaaaacgag tttacgcaat tgcacaatca tgctgactct gtggcggacc cgcgctcttg 6361 ccggcccggc gataacgctg ggcgtgaggc tgtgcccggc ggagtttttt gcgcctgcat 6421 tttccaaggt ttaccctgcg ctaaggggcg agattggaga agcaataaga atgccggttg 6481 gggttgcgat gatgacgacc acgacaactg gtgtcattat ttaagttgcc gaaagaacct 6541 gagtgcattt gcaacatgag tatactagaa gaatgagcca agacttgcga gacgcgagtt 6601 tgccggtggt gcgaacaata gagcgaccat gaccttgaag gtgagacgcg cataaccgct 6661 agagtacttt gaagaggaaa cagcaatagg gttgctacca gtataaatag acaggtacat 6721 acaacactgg aaatggttgt ctgtttgagt acgctttcaa ttcatttggg tgtgcacttt 6781 attatgttac aatatggaag ggaactttac acttctccta tgcacatata ttaattaaag 6841 tccaatgcta gtagagaagg ggggtaacac ccctccgcgc tcttttccga tttttttcta 6901 aaccgtggaa tatttcggat atccttttgt tgtttccggg tgtacaatat ggacttcctc 6961 ttttctggca accaaaccca tacatcggga ttcctataat accttcgttg gtctccctaa 7021 catgtaggtg gcggagggga gatatacaat agaacagata ccagacaaga cataatgggc 7081 taaacaagac tacaccaatt acactgcctc attgatggtg gtacataacg aactaatact 7141 gtagccctag acttgatagc catcatcata tcgaagtttc actacccttt ttccatttgc 7201 catctattga agtaataata ggcgcatgca acttcttttc tttttttttc ttttctctct 7261 cccccgttgt tgtctcacca tatccgcaat gacaaaaaaa tgatggaaga cactaaagga 7321 aaaaattaac gacaaagaca gcaccaacag atgtcgttgt tccagagctg atgaggggta 7381 tctcgaagca cacgaaactt tttccttcct tcattcacgc acactactct ctaatgagca 7441 acggtatacg gccttccttc cagttacttg aatttgaaat aaaaaaaagt ttgctgtctt 7501 gctatcaagt ataaatagac ctgcaattat taatcttttg tttcctcgtc attgttctcg 7561 ttccctttct tccttgtttc tttttctgca caatatttca agctatacca agcatacaat 7621 caactatctc atatacaatg ctcgagggag //