BBa_K1064007 1 BBa_K1064007 adh 2013-11-01T12:00:00Z 2015-05-08T01:08:59Z ralstonia adh false false _1372_ 0 15810 9 Not in stock false no false Ari Dwijayanti BBa_K1064008 1 BBa_K1064008 comp 2013-11-01T12:00:00Z 2015-05-08T01:08:59Z comp comp false false _1372_ 0 15810 9 Not in stock false comp false Ari Dwijayanti component2371240 1 BBa_K1064007 component2371238 1 BBa_B0030 component2371245 1 BBa_B1006 annotation2371238 1 BBa_B0030 range2371238 1 1 15 annotation2371245 1 BBa_B1006 range2371245 1 780 818 annotation2371240 1 BBa_K1064007 range2371240 1 22 771 BBa_B0030 1 BBa_B0030 RBS.1 (strong) -- modified from R. Weiss 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>. false true _44_46_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix (&quot;orig&quot; in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation1702 1 RBS range1702 1 8 12 annotation7025 1 BBa_B0030 range7025 1 1 15 annotation1701 1 RBS-1\Strong range1701 1 1 15 BBa_B1006 1 BBa_B1006 Terminator (artificial, large, %T~>90) 2006-08-30T11:00:00Z 2015-08-31T04:07:21Z modified E. coli thr terminator, replaced all A-T pairs in stem with C-G pairs Released HQ 2013 Artificial terminator, estimated %T~>90% *8bp stem, 6nt loop *Bidirectional, estimated reverse %T~>90% false true _41_ 0 745 41 In stock false Bidirectional, with the reverse estimated to be less effective than the forward. Has a polyA tail of 9 residues. true Haiyao Huang annotation1898431 1 PolyA range1898431 1 1 9 annotation1898430 1 PolyA range1898430 1 32 39 annotation1898429 1 modified thr terminator range1898429 1 10 31 annotation1898428 1 B1006 range1898428 1 1 39 BBa_K1064007_sequence 1 atgtatcgactattaaacaaaacagccgtcataaccggtggagggagcggcattggcctcgccacagcgaagcgcttcgttgccgagggtgcctatgtattcattgtcgatgtaagtcggaaggaactcgagcaggcggccgcagaaatcggtcggaatgtcacggcggtcaaagccgatgtgacaaagcttgaagacctggaccgactttacgcgattgtgcgtgagcaacggggtagcatcgacgtactatttaacaatgcaggcgcaatcgagcaaaagacgcttgaggagattactccggaacactatgacaggactttcgatgtcaacgttcggggattgatcttcaccgtgcagaaggcacttcctctgctgcgagacggcggcagcgtgatcctgacaagctcggtagccggcgtcctaggattacaggcgcacgacacgtatagtgccgccaaggcagcggtaaggtcgctcgcgaggacatggaccactgagttgaaaggtcgcagcattcgtgtcaacgcggtaagcccaggggcgatcgacacgcctatcatagaaaaccaggtctctacacaggaagaagctgacgagctgcgtgcgaaatttgcagctgcgacgcccctgggtcgcgtcggacgacctgaagagctggcagcggccgtgttatttcttgcatcggacgacagtagctacgtagccggcattgagctgtttgtggacggtggattgacccaggtctaa BBa_K1064008_sequence 1 attaaagaggagaaatactagatgtatcgactattaaacaaaacagccgtcataaccggtggagggagcggcattggcctcgccacagcgaagcgcttcgttgccgagggtgcctatgtattcattgtcgatgtaagtcggaaggaactcgagcaggcggccgcagaaatcggtcggaatgtcacggcggtcaaagccgatgtgacaaagcttgaagacctggaccgactttacgcgattgtgcgtgagcaacggggtagcatcgacgtactatttaacaatgcaggcgcaatcgagcaaaagacgcttgaggagattactccggaacactatgacaggactttcgatgtcaacgttcggggattgatcttcaccgtgcagaaggcacttcctctgctgcgagacggcggcagcgtgatcctgacaagctcggtagccggcgtcctaggattacaggcgcacgacacgtatagtgccgccaaggcagcggtaaggtcgctcgcgaggacatggaccactgagttgaaaggtcgcagcattcgtgtcaacgcggtaagcccaggggcgatcgacacgcctatcatagaaaaccaggtctctacacaggaagaagctgacgagctgcgtgcgaaatttgcagctgcgacgcccctgggtcgcgtcggacgacctgaagagctggcagcggccgtgttatttcttgcatcggacgacagtagctacgtagccggcattgagctgtttgtggacggtggattgacccaggtctaatactagagaaaaaaaaaccccgcccctgacagggcggggtttttttt BBa_B0030_sequence 1 attaaagaggagaaa BBa_B1006_sequence 1 aaaaaaaaaccccgcccctgacagggcggggtttttttt igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z