LOCUS BBa_K1065201 5680 bp DNA linear 14-Jun-2024 DEFINITION Pxyl+GFP in an integrative Bacillus subtilis specific backbone ACCESSION BBa_K1065201 VERSION BBa_K1065201.1 FEATURES Location/Qualifiers CDS 211..885 /label=spc misc_feature 1028..1456 /label='thrC misc_feature 1453..1863 /label=thrB CDS 2716..3576 /label=bla misc_feature 3731..4033 /label=hom misc_feature 4033..4572 /label=thrC' promoter 4539..4763 /label=Pxly region misc_feature 1..4794 /label=BBa_K823024 RBS 4803..4817 /label=RBS-1\Strong RBS 4810..4814 /label=RBS misc_feature 4803..4817 /label=BBa_B0030 RBS 4803..4817 /label=BBa_B0030 CDS 4824..5543 /label=GFP protein CDS 4824..5543 /label=BBa_E0040 stem_loop 5563..5606 /label=stem_loop misc_feature 5552..5631 /label=BBa_B0010 terminator 5552..5631 /label=BBa_B0010 stem_loop 5647..5666 /label=T7 TE misc_feature 5673..5673 /label=stop polyA_site 5667..5680 /label=polya misc_feature 5640..5680 /label=BBa_B0012 terminator 5640..5680 /label=BBa_B0012 misc_feature 4803..5680 /label=BBa_E0840 ORIGIN 1 tactagtagc ggccgctgca ggataaaaaa tttagaagcc aatgaaatct ataaataaac 61 taaattaagt ttatttaatt aacaactatg gatataaaat aggtactaat caaaatagtg 121 aggaggatat atttgaatac atacgaacaa attaataaag tgaaaaaaat acttcggaaa 181 catttaaaaa ataaccttat tggtacttac atgtttggat caggagttga gagtggacta 241 aaaccaaata gtgatcttga ctttttagtc gtcgtatctg aaccattgac agatcaaagt 301 aaagaaatac ttatacaaaa aattagacct atttcaaaaa aaataggaga taaaagcaac 361 ttacgatata ttgaattaac aattattatt cagcaagaaa tggtaccgtg gaatcatcct 421 cccaaacaag aatttattta tggagaatgg ttacaagagc tttatgaaca aggatacatt 481 cctcagaagg aattaaattc agatttaacc ataatgcttt accaagcaaa acgaaaaaat 541 aaaagaatat acggaaatta tgacttagag gaattactac ctgatattcc attttctgat 601 gtgagaagag ccattatgga ttcgtcagag gaattaatag ataattatca ggatgatgaa 661 accaactcta tattaacttt atgccgtatg attttaacta tggacacggg taaaatcata 721 ccaaaagata ttgcgggaaa tgcagtggct gaatcttctc cattagaaca tagggagaga 781 attttgttag cagttcgtag ttatcttgga gagaatattg aatggactaa tgaaaatgta 841 aatttaacta taaactattt aaataacaga ttaaaaaaat tataaaaaaa ttgaaaaaat 901 ggtggaaaca cttttttcaa tttttttgtt ttattattta atatttggga aatattcatt 961 ctaattggta atcagatttt agaaaacaat aaacccttgc atagggggat ctcgaggcct 1021 tccgaaaatg cgcggctttg aagctgaggg tgcggcggca atcgtgcgca atgaagtgat 1081 tgaaaatccg gaaacaatag cgacagccat tcgtatcgga aacccggcaa gctgggacaa 1141 agctgtaaag gcagccgagg aatccaatgg gaaaattgac gaagtcactg atgatgaaat 1201 ccttcacgca tatcagctga tcgcccgtgt agaaggcgtg tttgcagaac caggttcttg 1261 cgcgtctatc gcaggagtgc tgaaacaggt gaaatccgga gaaattccga aaggcagcaa 1321 ggtcgtagct gtgttaacag gaaacggact gaaagatccg aacacagcgg tcgacatttc 1381 agaaatcaag cctgtcacat tgccgactga tgaagacagc atccttgaat atgtaaaagg 1441 agcggcccgt gtatgaacga agccgacatg ctgttctctg tcactgttcc cggaagcaca 1501 gctaacctag gccccggctt tgattcagtc ggaatggcgc tcagcagata tttgaagctg 1561 accgtctttg aaagcgacaa atggtctttt gaggctgaaa cagaaacagt cgccggaatt 1621 aattcgagct cctttaactt cctcaccagt agttgtatcg gtaccataag tagaagcagc 1681 aacccaagta gctttaccag catccggttc aaccagcata gtaagaatct tactggacat 1741 cggcagttct tcgaacagtg cgccaactac cagctctttc tacagttcat tcagggcacc 1801 ggagaacctg cgtgcaatcc atcttgttca atcatgcgaa acgatcctca tcctgtctct 1861 tgatccatgg attacgcgcg accttcagca tcgccggcat gtccccctgg cggacgggaa 1921 gtatccagct cgaggtcggg ccgcgttgct ggcgtttttc cataggctcc gcccccctga 1981 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag 2041 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct 2101 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg 2161 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc 2221 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt 2281 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta 2341 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac 2401 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttgatagctc 2461 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat 2521 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc 2581 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt 2641 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta 2701 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct 2761 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg 2821 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga 2881 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt 2941 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt 3001 taatagtttg cgcaacgttg ttgccattgc tgccggcatc gtggtgtcac gctcgtcgtt 3061 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat 3121 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc 3181 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc 3241 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat 3301 gcggcgaccg agttgctctt gcccggcgtc aacacgggat aataccgcgc cacatagcag 3361 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt 3421 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc 3481 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa 3541 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg 3601 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa 3661 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac 3721 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtcttca 3781 agaattaatt catgtaaaag atgaggttgg ttcattctcg aaaattacat ctgtgttctc 3841 agagcggggc gtgacgtttg aaaaaatcct tcagctgcca attaaaggcc atgatgagtt 3901 agctgaaatc gtaattgtca cacatcatac atcagaagct gatttcagtg atatcctgca 3961 aaacctaaat gatttggaag tcgttcaaga agtcaaaagc acatatcgtg tagaagggaa 4021 cggttggagc taatgtggaa aggacttatc catcaatata aagaattttt acctgtaaca 4081 gatcaaacac cggcgctaac tttacatgaa ggaaacacac ctcttattca cctgccgaag 4141 ctgtctgagc agctcggaat tgagcttcat gtcaaaacgg aaggcgtcaa tcctacggga 4201 tcatttaaag atcgcggaat ggttatggct gtggcaaagg caaaagaaga aggcaatgac 4261 acgattatgt gcgcgtcaac aggtaacact tccgctgctg cggcagcata tgcagcccgt 4321 gctaacatga aatgcattgt catcatcccg aacggaaaaa ttgcatttgg aaaactcgct 4381 caagctgtca tgtacggagc cgagattatc gcaattgacg gaaactttga cgatgcgctt 4441 aaaattgtcc gttccatctg tgagaaatca ccgattgccc ttgtcaactc agtcaaccct 4501 taccgcattg aaggccaaaa aactgctgcc ttcggatcag cgatatccac ttcatccact 4561 ccatttgttt aatctttaaa ttaagtatca acatagtaca tagcgaatct tccctttatt 4621 atatctaatg tgttcataaa aaactaaaaa aaatattgaa aatactgacg aggttatata 4681 agatgaaaat aagttagttt gtttaaacaa caaactaata ggtgatgtac ttactatatg 4741 aaataaaatg catctgggat cccaagctta tcgaattcgc ggccgcttct agagtactag 4801 agattaaaga ggagaaatac tagatgcgta aaggagaaga acttttcact ggagttgtcc 4861 caattcttgt tgaattagat ggtgatgtta atgggcacaa attttctgtc agtggagagg 4921 gtgaaggtga tgcaacatac ggaaaactta cccttaaatt tatttgcact actggaaaac 4981 tacctgttcc atggccaaca cttgtcacta ctttcggtta tggtgttcaa tgctttgcga 5041 gatacccaga tcatatgaaa cagcatgact ttttcaagag tgccatgccc gaaggttatg 5101 tacaggaaag aactatattt ttcaaagatg acgggaacta caagacacgt gctgaagtca 5161 agtttgaagg tgataccctt gttaatagaa tcgagttaaa aggtattgat tttaaagaag 5221 atggaaacat tcttggacac aaattggaat acaactataa ctcacacaat gtatacatca 5281 tggcagacaa acaaaagaat ggaatcaaag ttaacttcaa aattagacac aacattgaag 5341 atggaagcgt tcaactagca gaccattatc aacaaaatac tccaattggc gatggccctg 5401 tccttttacc agacaaccat tacctgtcca cacaatctgc cctttcgaaa gatcccaacg 5461 aaaagagaga ccacatggtc cttcttgagt ttgtaacagc tgctgggatt acacatggca 5521 tggatgaact atacaaataa taatactaga gccaggcatc aaataaaacg aaaggctcag 5581 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt 5641 cacactggct caccttcggg tgggcctttc tgcgtttata //