LOCUS       BBa_K1072006            1883 bp    DNA     linear       21-May-2024
DEFINITION  Lux pR+RBS+α-ALS+2TM
ACCESSION   BBa_K1072006
VERSION     BBa_K1072006.1  
FEATURES             Location/Qualifiers
     misc_feature    1..20
                     /label=LuxR/HSL
     promoter        20..25
                     /label=-35
     promoter        42..47
                     /label=-10
     misc_feature    53..53
                     /label=start
     misc_feature    1..55
                     /label=BBa_R0062
     promoter        1..55
                     /label=BBa_R0062
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     CDS             82..1746
                     /label=alpha-ALS
     CDS             82..1746
                     /label=BBa_K1072004
     stem_loop       1766..1809
                     /label=stem_loop
     misc_feature    1755..1834
                     /label=BBa_B0010
     terminator      1755..1834
                     /label=BBa_B0010
     stem_loop       1850..1869
                     /label=T7 TE
     misc_feature    1876..1876
                     /label=stop
     polyA_site      1870..1883
                     /label=polya
     misc_feature    1843..1883
                     /label=BBa_B0012
     terminator      1843..1883
                     /label=BBa_B0012
ORIGIN
        1 acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga ataaatacta
       61 gagaaagagg agaaatacta gatgtctgag aaacaatttg gggcgaactt ggttgtcgat
      121 agtttgatta accataaagt gaagtatgta tttgggattc caggagcaaa aattgaccgg
      181 gtttttgatt tattagaaaa tgaagaaggc cctcaaatgg tcgtgactcg tcatgagcaa
      241 ggagctgctt tcatggctca agctgttggt cgtttaactg gcgaacctgg tgtagtagtt
      301 gttacgagtg ggcctggtgt atcaaacctt gcgactccgc ttttaaccgc gacatcagaa
      361 ggtgatgcta ttttggctat cggtggacaa gttaaacgaa gtgaccgtct taaacgtgca
      421 catcaatcaa tggataatgc tggaatgatg caatcagcaa caaaatattc agcagaagtt
      481 cttgaccctg atacactttc tgaatcaatt gccaacgctt atcgtattgc aaaatcagga
      541 catccaggtg caactttctt atcaatcccc caagatataa cggatgccga agtatcaatc
      601 aaggccattc aaccactttc agaccctaaa atggggaatg cctctattga tgacattaac
      661 tatttagcac aagcaattaa aaatgctgta ttgccagtaa ttttggttgg agctggtgct
      721 tcagatgcta aagtcgcttc atcattgcgt aatctattga ctcatgttaa tattcctgtc
      781 gttgaaacat tccaaggtgc aggggttatt tcacatgatt tagaacatac tttttatgga
      841 cgtatcggac ttttccgcaa tcaaccagga gatatgcttt tgaagcgttc tgaccttgtt
      901 attgctgttg gttacgaccc aattgaatat gaagctcgta actggaatgc agaaattgat
      961 agtcgaatta tcgttattga taatgccatt gctgaaattg atacttacta ccaaccagag
     1021 cgtgaattaa ttggtgatat cgcagcaaca ttggataatc ttttaccggc tgttcgtggc
     1081 tacaaaattc caaaaggaac aaaagattat ctcgatgggc ttcatgaagt tgctgagcaa
     1141 cacgaatttg atactgaaaa tactgaagaa ggtagaatgc accctcttga tttggtcagc
     1201 actttccaag aaatcgtcaa agatgatgaa acagtaaccg ttgacgtagg ttcactctac
     1261 atttggatgg cacgtcattt caaatcatac gaaccacgtc atctcctctt ctcaaacgga
     1321 atgcaaacac ttggagttgc acttccttgg gcaattacag ccgcattgtt gcgcccaggt
     1381 aaaaaagttt attcacactc tggtgatgga ggtttccttt tcacagggca agaattggaa
     1441 acagctgtac gtttgaatct tccaatcgtt caaattatct ggaatgacgg ccattatgat
     1501 atggttaaat tccaagaaga aatgaaatat ggtcgttcag cagccgttga ttttggctat
     1561 gttgattacg taaaatatgc tgaagcaatg ggagcaaaag gttaccgtgc acacagcaaa
     1621 gaagaacttg ctgaaattct taaatcaatc ccagatacta ctggaccagt agtaattgac
     1681 gttcctttgg actattctga taacattaaa ttagcagaaa aattattgcc agaagaattt
     1741 tactgatact agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt
     1801 tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc
     1861 gggtgggcct ttctgcgttt ata
//