LOCUS       BBa_K1073018             823 bp    DNA     linear       15-May-2024
DEFINITION  Autoinducer synthetase LasI ( producing N-3-oxododecanoyl-HSL) 
            with RBS and double terminator
ACCESSION   BBa_K1073018
VERSION     BBa_K1073018.1  
FEATURES             Location/Qualifiers
     RBS             1..13
                     /label=RBS-3\Weak
     RBS             7..10
                     /label=RBS
     misc_feature    1..13
                     /label=BBa_B0032
     RBS             1..13
                     /label=BBa_B0032
     CDS             20..622
                     /label=LasI
     misc_feature    623..655
                     /label=LVA
     misc_feature    662..686
                     /label=Help:Barcodes
     CDS             20..686
                     /label=BBa_C0078
     stem_loop       706..749
                     /label=stem_loop
     misc_feature    695..774
                     /label=BBa_B0010
     terminator      695..774
                     /label=BBa_B0010
     stem_loop       790..809
                     /label=T7 TE
     misc_feature    816..816
                     /label=stop
     polyA_site      810..823
                     /label=polya
     misc_feature    783..823
                     /label=BBa_B0012
     terminator      783..823
                     /label=BBa_B0012
     terminator      695..823
                     /label=BBa_B0015
ORIGIN
        1 tcacacagga aagtactaga tgatcgttca gatcggtcgt cgtgaagagt tcgacaaaaa
       61 actgctgggt gaaatgcaca aactgcgtgc tcaggttttc aaagaacgta aaggttggga
      121 cgtttccgtt atcgacgaaa tggaaatcga cggttacgac gctctgtccc cgtactacat
      181 gctgatccag gaagacaccc cggaagctca ggttttcggt tgctggcgta tcttcgacac
      241 caccggtccg tacatgctga aaaacacctt cccggaactg ctgcacggta aagaagctcc
      301 gtgctccccg cacatctggg aactgtcccg tttcgctatc aactccggtc agaaaggttc
      361 cctgggtttc tccgactgca ccctggaagc tatgcgtgct ctggctcgtt actccttgca
      421 gaacgacatc cagaccctgg ttaccgttac caccgttggt gttgaaaaaa tgatgatccg
      481 tgctggtctg gacgtttccc gtttcggtcc gcacctgaaa atcggtatcg aacgtgctgt
      541 tgctctgcgt atcgaactga acgctaaaac ccagatcgct ctgtacggtg gtgttctggt
      601 tgaacagcgt ctggctgttt ccgctgctaa cgacgaaaac tacgctctgg ttgcttaata
      661 actctgatag tgctagtgta gatctctact agagccaggc atcaaataaa acgaaaggct
      721 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag
      781 agtcacactg gctcaccttc gggtgggcct ttctgcgttt ata
//