LOCUS       BBa_K1073030            2005 bp    DNA     linear       14-Jun-2024
DEFINITION  Inducible ampicillin resistance cassette + LasR expression cassette
ACCESSION   BBa_K1073030
VERSION     BBa_K1073030.1  
FEATURES             Location/Qualifiers
     misc_feature    86..91
                     /label=-35 Region
     misc_feature    75..93
                     /label=LasR Binding Site
     misc_feature    113..118
                     /label= -10 Region
     misc_feature    125..125
                     /label=Primary Transcriptional Start
     promoter        1..126
                     /label=BBa_K091117
     RBS             135..147
                     /label=RBS-3\Weak
     RBS             141..144
                     /label=RBS
     misc_feature    135..147
                     /label=BBa_B0032
     RBS             135..147
                     /label=BBa_B0032
     promoter        1..147
                     /label=BBa_K1073003
     CDS             154..1017
                     /label=BBa_K1073000
     misc_feature    1..1017
                     /label=BBa_K1073013
     stem_loop       1037..1080
                     /label=stem_loop
     misc_feature    1026..1105
                     /label=BBa_B0010
     terminator      1026..1105
                     /label=BBa_B0010
     stem_loop       1121..1140
                     /label=T7 TE
     misc_feature    1147..1147
                     /label=stop
     polyA_site      1141..1154
                     /label=polya
     misc_feature    1114..1154
                     /label=BBa_B0012
     terminator      1114..1154
                     /label=BBa_B0012
     terminator      1026..1154
                     /label=BBa_B0015
     promoter        1163..1197
                     /label=BBa_J23100
     RBS             1206..1218
                     /label=RBS-3\Weak
     RBS             1212..1215
                     /label=RBS
     misc_feature    1206..1218
                     /label=BBa_B0032
     RBS             1206..1218
                     /label=BBa_B0032
     promoter        1163..1218
                     /label=BBa_K1073006
     CDS             1225..1941
                     /label=lasR
     misc_feature    1942..1974
                     /label=LVA
     misc_feature    1704..1704
                     /label=G
     misc_feature    1981..2005
                     /label=Help:Barcodes
     CDS             1225..2005
                     /label=BBa_C0079
     misc_feature    1163..2005
                     /label=BBa_K1073016
ORIGIN
        1 tgttctcgtg tgaagccatt gctctgatct tttggacgtt tcttcgagcc tagcaagggt
       61 ccgggttcac cgaaatctat ctcatttgct agttataaaa ttatgaaatt tgtataaatt
      121 cttcagtact agagtcacac aggaaagtac tagatgagta ttcaacattt ccgtgtcgcc
      181 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
      241 aaagtaaaag atgccgaaga tcagttgggt gcacgtgtgg gttacatcga actggacctc
      301 aacagcggta agattcttga gagttttcgc cccgaagaac gtttcccaat gatgagcact
      361 tttaaagttc tgctctgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
      421 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
      481 catcttacgg acggcatgac agtacgcgaa ttatgcagcg ctgccataac catgagtgat
      541 aacacggcgg ccaacttact tctgacaacg atcggaggac cgaaggagct taccgctttt
      601 ttgcacaaca tgggtgatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
      661 gccataccaa acgacgagcg tgacaccacg atgcctgtag ctatggcaac aacgttgcgc
      721 aaactcttaa ctggcgaact tcttactctc gcttcccggc aacaattaat agactggatg
      781 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
      841 gctgataaat ctggagccgg tgagcgtggg tcccgcggta ttattgcagc cctggggcca
      901 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagccaggc aactatggac
      961 gaacgtaatc gccagatcgc tgagataggt gcctccctga ttaagcattg gtaataatac
     1021 tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat
     1081 ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc
     1141 tttctgcgtt tatatactag agttgacggc tagctcagtc ctaggtacag tgctagctac
     1201 tagagtcaca caggaaagta ctagatggcc ttggttgacg gttttcttga gctggaacgc
     1261 tcaagtggaa aattggagtg gagcgccatc ctccagaaga tggcgagcga ccttggattc
     1321 tcgaagatcc tgttcggcct gttgcctaag gacagccagg actacgagaa cgccttcatc
     1381 gtcggcaact acccggccgc ctggcgcgag cattacgacc gggctggcta cgcgcgggtc
     1441 gacccgacgg tcagtcactg tacccagagc gtactgccga ttttctggga accgtccatc
     1501 taccagacgc gaaagcagca cgagttcttc gaggaagcct cggccgccgg cctggtgtat
     1561 gggctgacca tgccgctgca tggtgctcgc ggcgaactcg gcgcgctgag cctcagcgtg
     1621 gaagcggaaa accgggccga ggccaaccgt ttcatagagt cggtcctgcc gaccctgtgg
     1681 atgctcaagg actacgcact gcaaagcggt gccggactgg ccttcgaaca tccggtcagc
     1741 aaaccggtgg ttctgaccag ccgggagaag gaagtgttgc agtggtgcgc catcggcaag
     1801 accagttggg agatatcggt tatctgcaac tgctcggaag ccaatgtgaa cttccatatg
     1861 ggaaatattc ggcggaagtt cggtgtgacc tcccgccgcg tagcggccat tatggccgtt
     1921 aatttgggtc ttattactct cgctgcaaac gacgaaaact acgctttagt agcttaataa
     1981 cactgatagt gctagtgtag atcac
//