LOCUS       BBa_K1073032            1711 bp    DNA     linear       01-Jun-2024
DEFINITION  Autoinducer synthase LasI + LVA with combined eforRed 
            chromoprotein expression cassette
ACCESSION   BBa_K1073032
VERSION     BBa_K1073032.1  
FEATURES             Location/Qualifiers
     RBS             1..13
                     /label=RBS-3\Weak
     RBS             7..10
                     /label=RBS
     misc_feature    1..13
                     /label=BBa_B0032
     RBS             1..13
                     /label=BBa_B0032
     CDS             20..622
                     /label=LasI
     misc_feature    623..655
                     /label=LVA
     misc_feature    662..686
                     /label=Help:Barcodes
     CDS             20..686
                     /label=BBa_C0078
     stem_loop       706..749
                     /label=stem_loop
     misc_feature    695..774
                     /label=BBa_B0010
     terminator      695..774
                     /label=BBa_B0010
     stem_loop       790..809
                     /label=T7 TE
     misc_feature    816..816
                     /label=stop
     polyA_site      810..823
                     /label=polya
     misc_feature    783..823
                     /label=BBa_B0012
     terminator      783..823
                     /label=BBa_B0012
     terminator      695..823
                     /label=BBa_B0015
     misc_feature    1..823
                     /label=BBa_K1073018
     promoter        832..866
                     /label=BBa_J23100
     RBS             875..887
                     /label=RBS-3\Weak
     RBS             881..884
                     /label=RBS
     misc_feature    875..887
                     /label=BBa_B0032
     RBS             875..887
                     /label=BBa_B0032
     promoter        832..887
                     /label=BBa_K1073006
     CDS             894..1574
                     /label=eforRed
     CDS             894..1574
                     /label=BBa_K592012
     misc_feature    832..1574
                     /label=BBa_K1073022
     stem_loop       1594..1637
                     /label=stem_loop
     misc_feature    1583..1662
                     /label=BBa_B0010
     terminator      1583..1662
                     /label=BBa_B0010
     stem_loop       1678..1697
                     /label=T7 TE
     misc_feature    1704..1704
                     /label=stop
     polyA_site      1698..1711
                     /label=polya
     misc_feature    1671..1711
                     /label=BBa_B0012
     terminator      1671..1711
                     /label=BBa_B0012
     terminator      1583..1711
                     /label=BBa_B0015
ORIGIN
        1 tcacacagga aagtactaga tgatcgttca gatcggtcgt cgtgaagagt tcgacaaaaa
       61 actgctgggt gaaatgcaca aactgcgtgc tcaggttttc aaagaacgta aaggttggga
      121 cgtttccgtt atcgacgaaa tggaaatcga cggttacgac gctctgtccc cgtactacat
      181 gctgatccag gaagacaccc cggaagctca ggttttcggt tgctggcgta tcttcgacac
      241 caccggtccg tacatgctga aaaacacctt cccggaactg ctgcacggta aagaagctcc
      301 gtgctccccg cacatctggg aactgtcccg tttcgctatc aactccggtc agaaaggttc
      361 cctgggtttc tccgactgca ccctggaagc tatgcgtgct ctggctcgtt actccttgca
      421 gaacgacatc cagaccctgg ttaccgttac caccgttggt gttgaaaaaa tgatgatccg
      481 tgctggtctg gacgtttccc gtttcggtcc gcacctgaaa atcggtatcg aacgtgctgt
      541 tgctctgcgt atcgaactga acgctaaaac ccagatcgct ctgtacggtg gtgttctggt
      601 tgaacagcgt ctggctgttt ccgctgctaa cgacgaaaac tacgctctgg ttgcttaata
      661 actctgatag tgctagtgta gatctctact agagccaggc atcaaataaa acgaaaggct
      721 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag
      781 agtcacactg gctcaccttc gggtgggcct ttctgcgttt atatactaga gttgacggct
      841 agctcagtcc taggtacagt gctagctact agagtcacac aggaaagtac tagatgtcag
      901 tgattaagca ggtaatgaag accaagttgc accttgaggg cactgtcaat ggccatgatt
      961 ttacgatcga gggtaaaggt gaaggcaagc cgtacgaagg gttacagcac atgaaaatga
     1021 cagtcaccaa aggcgcgcct ctgccgtttt ccgttcatat tcttacacct agccacatgt
     1081 atggaagcaa accgtttaat aagtatccag cggatatccc agactaccac aaacagtctt
     1141 ttcccgaagg tatgtcttgg gagcggtcga tgatttttga agatggtggc gtatgcaccg
     1201 ccagtaatca ctccagcata aacttgcaag agaactgttt catctatgat gttaaatttc
     1261 atggtgtgaa cctgcctccg gatgggcccg taatgcaaaa aaccattgct ggatgggagc
     1321 cgagcgtgga aacactgtac gtgcgtgacg ggatgttaaa aagtgacact gcaatggttt
     1381 ttaaactgaa aggaggcggt catcatcgtg ttgatttcaa aacgacgtat aaagccaaaa
     1441 aacctgtcaa gctgccagaa tttcatttcg ttgaacatcg cctggaactg accaaacacg
     1501 ataaagattt cacaacttgg gaccagcagg aggcagccga aggccatttc tcaccgctgc
     1561 cgaaggctct cccatactag agccaggcat caaataaaac gaaaggctca gtcgaaagac
     1621 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc
     1681 tcaccttcgg gtgggccttt ctgcgtttat a
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