LOCUS       BBa_K1075006             717 bp    DNA     linear       17-May-2024
DEFINITION  pLac-1-RBS32-sspB-TT
ACCESSION   BBa_K1075006
VERSION     BBa_K1075006.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     RBS             64..76
                     /label=RBS-3\Weak
     RBS             70..73
                     /label=RBS
     misc_feature    64..76
                     /label=BBa_B0032
     RBS             64..76
                     /label=BBa_B0032
     CDS             83..580
                     /label=sspB
     CDS             83..580
                     /label=BBa_K174000
     stem_loop       600..643
                     /label=stem_loop
     misc_feature    589..668
                     /label=BBa_B0010
     terminator      589..668
                     /label=BBa_B0010
     stem_loop       684..703
                     /label=T7 TE
     misc_feature    710..710
                     /label=stop
     polyA_site      704..717
                     /label=polya
     misc_feature    677..717
                     /label=BBa_B0012
     terminator      677..717
                     /label=BBa_B0012
     terminator      589..717
                     /label=BBa_B0015
     misc_feature    83..717
                     /label=BBa_K1075004
     misc_feature    64..717
                     /label=BBa_K1075005
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagtcacaca ggaaagtact agatggattt gtcacagcta acaccacgtc gtccctatct
      121 gctgcgtgca ttctatgagt ggttgctgga taaccagctc acgccgcacc tggtggtgga
      181 tgtgacgctc cctggcgtgc aggttcctat ggaatatgcg cgtgacgggc aaatcgtact
      241 caacattgcg ccgcgtgctg tcggcaatct ggaactggcg aatgatgagg tgcgctttaa
      301 cgcgcgcttt ggtggcattc cgcgtcaggt ttctgtgccg ctggctgccg tgctggctat
      361 ctacgcccgt gaaaatggcg caggcacgat gtttgagcct gaagctgcct acgatgaaga
      421 taccagcatc atgaatgatg aagaggcatc ggcagacaac gaaaccgtta tgtcggttat
      481 tgatggcgac aagccagatc acgatgatga cactcatcct gacgatgaac ctccgcagcc
      541 accacgcggt ggtcgaccgg cattacgcgt tgtgaagtaa tactagagcc aggcatcaaa
      601 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga
      661 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata
//