LOCUS       BBa_K1075025            1865 bp    DNA     linear       17-May-2024
DEFINITION  pLac-RBS34-mCherry-(Ec)ssrA(DAS+4)-TT
ACCESSION   BBa_K1075025
VERSION     BBa_K1075025.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     CDS             227..937
                     /label=mCherry
     misc_feature    578..578
                     /label=C->T (removing PstI site)
     misc_feature    227..940
                     /label=BBa_J06504
     CDS             947..1654
                     /label=mCherry
     misc_feature    1298..1298
                     /label=C->T (removing PstI site)
     misc_feature    1655..1663
                     /label=LVA
     misc_feature    947..1669
                     /label=BBa_J06505
     misc_feature    1678..1728
                     /label=BBa_K1075020
     stem_loop       1748..1791
                     /label=stem_loop
     misc_feature    1737..1816
                     /label=BBa_B0010
     terminator      1737..1816
                     /label=BBa_B0010
     stem_loop       1832..1851
                     /label=T7 TE
     misc_feature    1858..1858
                     /label=stop
     polyA_site      1852..1865
                     /label=polya
     misc_feature    1825..1865
                     /label=BBa_B0012
     terminator      1825..1865
                     /label=BBa_B0012
     terminator      1737..1865
                     /label=BBa_B0015
     misc_feature    947..1865
                     /label=BBa_K1075023
     misc_feature    209..1865
                     /label=BBa_K1075024
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgg tgagcaaggg
      241 cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg
      301 ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg
      361 cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat
      421 cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc
      481 cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga
      541 ggacggcggc gtggtgaccg tgacccagga ctcctccttg caggacggcg agttcatcta
      601 caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac
      661 catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga
      721 gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac
      781 ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga
      841 catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg
      901 ccactccacc ggcggcatgg acgagctgta caagtaataa tactagatgg tgagcaaggg
      961 cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc acatggaggg
     1021 ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg
     1081 cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat
     1141 cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg ccgacatccc
     1201 cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga
     1261 ggacggcggc gtggtgaccg tgacccagga ctcctccttg caggacggcg agttcatcta
     1321 caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc agaagaagac
     1381 catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc tgaagggcga
     1441 gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg tcaagaccac
     1501 ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca tcaagttgga
     1561 catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg
     1621 ccactccacc ggcggcatgg acgagctgta caagttagta gcttaataat actagaggca
     1681 gctaacgatg aaaactacag cgaaaactat gctgacgcta gctaataata ctagagccag
     1741 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
     1801 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt
     1861 ttata
//