LOCUS       BBa_K1075035             666 bp    DNA     linear       17-May-2024
DEFINITION  pLac-RBS32-ccdA-ssrA-TT
ACCESSION   BBa_K1075035
VERSION     BBa_K1075035.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     RBS             209..221
                     /label=RBS-3\Weak
     RBS             215..218
                     /label=RBS
     misc_feature    209..221
                     /label=BBa_B0032
     RBS             209..221
                     /label=BBa_B0032
     CDS             228..470
                     /label=BBa_K1075032
     misc_feature    479..529
                     /label=BBa_K1075020
     stem_loop       549..592
                     /label=stem_loop
     misc_feature    538..617
                     /label=BBa_B0010
     terminator      538..617
                     /label=BBa_B0010
     stem_loop       633..652
                     /label=T7 TE
     misc_feature    659..659
                     /label=stop
     polyA_site      653..666
                     /label=polya
     misc_feature    626..666
                     /label=BBa_B0012
     terminator      626..666
                     /label=BBa_B0012
     terminator      538..666
                     /label=BBa_B0015
     misc_feature    479..666
                     /label=BBa_K1075021
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagtc acacaggaaa gtactagatg aagcagcgca
      241 ttacagtcac cgtcgacagt gacagctatc agttgctcaa ggcatatgat gtcaatatct
      301 ccggtctggt aagcaccacc atgcagaatg aagcccgtcg tctgcgtgcc gaacgctgga
      361 aagcagaaaa tcaggaaggg atggctgagg tcgcccggtt tattgaaatg aacggctctt
      421 ttgccgatga gaacagggac tggtgaaatg cagtttaagg tttacaccta tactagaggc
      481 agctaacgat gaaaactaca gcgaaaacta tgctgacgct agctaataat actagagcca
      541 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt
      601 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg
      661 tttata
//