LOCUS       BBa_K1075038             495 bp    DNA     linear       18-May-2024
DEFINITION  RBS32-MazF-TT
ACCESSION   BBa_K1075038
VERSION     BBa_K1075038.1  
FEATURES             Location/Qualifiers
     RBS             1..13
                     /label=RBS-3\Weak
     RBS             7..10
                     /label=RBS
     misc_feature    1..13
                     /label=BBa_B0032
     RBS             1..13
                     /label=BBa_B0032
     misc_feature    353..358
                     /label=double TAA added
     CDS             20..358
                     /label=BBa_K302033
     stem_loop       378..421
                     /label=stem_loop
     misc_feature    367..446
                     /label=BBa_B0010
     terminator      367..446
                     /label=BBa_B0010
     stem_loop       462..481
                     /label=T7 TE
     misc_feature    488..488
                     /label=stop
     polyA_site      482..495
                     /label=polya
     misc_feature    455..495
                     /label=BBa_B0012
     terminator      455..495
                     /label=BBa_B0012
     terminator      367..495
                     /label=BBa_B0015
     misc_feature    20..495
                     /label=BBa_K1075037
ORIGIN
        1 tcacacagga aagtactaga tggtaagccg atacgtaccc gatatgggcg atctgatttg
       61 ggttgatttt gacccgacaa aaggtagcga gcaagctgga catcgtccag ctgttgtcct
      121 gagtcctttc atgtacaaca acaaaacagg tatgtgtctg tgtgttcctt gtacaacgca
      181 atcaaaagga tatccgttcg aagttgtttt atccggtcag gaacgtgatg gcgtagcgtt
      241 agctgatcag gtaaaaagta tcgcctggcg ggcaagagga gcaacgaaga aaggaacagt
      301 tgccccagag gaattacaac tcattaaagc caaaattaac gtactgattg ggtaataata
      361 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta
      421 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc
      481 ctttctgcgt ttata
//