LOCUS       BBa_K1075039             558 bp    DNA     linear       17-May-2024
DEFINITION  pLac-RBS32-mazF-TT
ACCESSION   BBa_K1075039
VERSION     BBa_K1075039.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     RBS             64..76
                     /label=RBS-3\Weak
     RBS             70..73
                     /label=RBS
     misc_feature    64..76
                     /label=BBa_B0032
     RBS             64..76
                     /label=BBa_B0032
     misc_feature    416..421
                     /label=double TAA added
     CDS             83..421
                     /label=BBa_K302033
     stem_loop       441..484
                     /label=stem_loop
     misc_feature    430..509
                     /label=BBa_B0010
     terminator      430..509
                     /label=BBa_B0010
     stem_loop       525..544
                     /label=T7 TE
     misc_feature    551..551
                     /label=stop
     polyA_site      545..558
                     /label=polya
     misc_feature    518..558
                     /label=BBa_B0012
     terminator      518..558
                     /label=BBa_B0012
     terminator      430..558
                     /label=BBa_B0015
     misc_feature    83..558
                     /label=BBa_K1075037
     misc_feature    64..558
                     /label=BBa_K1075038
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagtcacaca ggaaagtact agatggtaag ccgatacgta cccgatatgg gcgatctgat
      121 ttgggttgat tttgacccga caaaaggtag cgagcaagct ggacatcgtc cagctgttgt
      181 cctgagtcct ttcatgtaca acaacaaaac aggtatgtgt ctgtgtgttc cttgtacaac
      241 gcaatcaaaa ggatatccgt tcgaagttgt tttatccggt caggaacgtg atggcgtagc
      301 gttagctgat caggtaaaaa gtatcgcctg gcgggcaaga ggagcaacga agaaaggaac
      361 agttgcccca gaggaattac aactcattaa agccaaaatt aacgtactga ttgggtaata
      421 atactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt
      481 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg
      541 ggcctttctg cgtttata
//