LOCUS       BBa_K1075043             672 bp    DNA     linear       17-May-2024
DEFINITION  pLac-RBS32-mazE-(Ec)ssrA(DAS+4)-TT
ACCESSION   BBa_K1075043
VERSION     BBa_K1075043.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     RBS             209..221
                     /label=RBS-3\Weak
     RBS             215..218
                     /label=RBS
     misc_feature    209..221
                     /label=BBa_B0032
     RBS             209..221
                     /label=BBa_B0032
     CDS             228..476
                     /label=BBa_K302032
     misc_feature    485..535
                     /label=BBa_K1075020
     misc_feature    209..535
                     /label=BBa_K1075041
     stem_loop       555..598
                     /label=stem_loop
     misc_feature    544..623
                     /label=BBa_B0010
     terminator      544..623
                     /label=BBa_B0010
     stem_loop       639..658
                     /label=T7 TE
     misc_feature    665..665
                     /label=stop
     polyA_site      659..672
                     /label=polya
     misc_feature    632..672
                     /label=BBa_B0012
     terminator      632..672
                     /label=BBa_B0012
     terminator      544..672
                     /label=BBa_B0015
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagtc acacaggaaa gtactagatg atccacagta
      241 gcgtaaagcg ttggggaaat tcaccggcgg tgcggatccc ggctacgtta atgcaggcgc
      301 tcaatctgaa tattgatgat gaagtgaaga ttgacctggt ggatggcaaa ttaattattg
      361 agccagtgcg taaagagccc gtatttacgc ttgctgaact ggtcaacgac atcacgccgg
      421 aaaacctcca cgagaatatc gactggggag agccgaaaga taaggaagtc tggtaatact
      481 agaggcagct aacgatgaaa actacagcga aaactatgct gacgctagct aataatacta
      541 gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
      601 gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt
      661 tctgcgttta ta
//