LOCUS       BBa_K1080018            2223 bp    DNA     linear       19-May-2024
DEFINITION  ChlD (+ Ptac promoter)
ACCESSION   BBa_K1080018
VERSION     BBa_K1080018.1  
FEATURES             Location/Qualifiers
     promoter        1..40
                     /label=Ptac
     promoter        7..12
                     /label=-35
     promoter        29..33
                     /label=-10
     misc_feature    36..61
                     /label=lacO1 site
     promoter        1..61
                     /label=BBa_K864400
     RBS             75..85
                     /label=RBS
     CDS             94..2223
                     /label=ChlD
     CDS             70..2223
                     /label=BBa_K1080002
ORIGIN
        1 gagctgttga caattaatca tcggctcgta taatgtgtgg aattgtgagc ggataacaat
       61 ttactagaga gctttaagaa ggagatatac ataatgcgcg ccatgaaggt gtctgaggag
      121 gactccaagg gcttcgatgc ggatgtgtcg acccgcctgg cccgctcgta ccctctggcg
      181 gccgtggtgg gccaggacaa catcaagcag gcgctgctgc tgggcgccgt ggacaccggg
      241 ctgggcggca tcgccatcgc cggtcgccgc ggtaccgcca agtccatcat ggctcgcggc
      301 ctgcacgctc tgctgccgcc cattgaggtg gtggagggca gcatctgcaa cgccgacccc
      361 gaggaccccc gctcctggga ggctggcctg gctgagaagt atgcgggcgg ccctgtgaag
      421 accaagatgc gctcggcgcc gtttgtgcag atccctctgg gtgtgactga ggaccgcttg
      481 gtgggcactg tggacattga ggcgtccatg aaggagggca agactgtgtt ccagcccggc
      541 ctgctggctg aggcgcaccg cggcatcctg tacgtggacg agatcaacct gctggatgac
      601 ggcattgcca acctgctgct gtccatcctg tcggacggag tcaacgtggt ggagcgcgag
      661 ggcatctcca tcagccaccc ctgccggccg ctgctgattg ccacctacaa ccccgaggag
      721 ggccctctgc gtgagcacct gctggaccgc atcgccattg gcctcagcgc cgacgtcccc
      781 agcaccagcg acgagcgcgt caaggccatt gacgcagcca tccgcttcca ggacaagccg
      841 caggacacta ttgacgacac cgcggagctc accgacgccc tgcgcacctc ggtcatcctg
      901 gctcgcgagt acctgaagga cgtgaccatc gcgccggagc aggtgaccta cattgtggag
      961 gaggcgcgcc gcggcggagt ccaggggcac cgcgcggagc tgtacgcggt caagtgtgcc
     1021 aaggcgtgtg cggctctgga gggccgtgag cgtgtgaaca aggatgacct gcgccaggcc
     1081 gtgcagctgg tcatcctgcc gcgcgccacc atcctggacc agcccccgcc cgagcaggag
     1141 cagcccccgc cgccgccccc gccccctccc ccgccgccgc cgcaggacca aatggaggac
     1201 gaggaccagg aggagaagga ggacgagaag gaggaggagg agaaggagaa cgaggaccag
     1261 gacgagcccg agatccctca ggagttcatg tttgagtccg agggcgtcat catggacccc
     1321 tccatcctca tgttcgcgca gcagcagcag cgcgcgcagg gccgctccgg ccgcgccaag
     1381 acgctcatct tcagcgacga ccgcggccgc tacatcaagc ccatgctgcc caagggtgac
     1441 aaggtcaagc gcctggcagt ggacgccacg cttcgcgccg ccgcgcccta ccagaagatt
     1501 cgccggcagc aggccatcag cgagggcaag gtgcagcgca aggtgtacgt ggacaagcca
     1561 gacatgcgct ccaagaagct ggcccgcaag gccggtgcgc tggtgatttt tgttgtggac
     1621 gcgtccggct ccatggctct gaaccgcatg agcgccgcca agggcgcctg catgcgcctg
     1681 ctggctgagt cgtacaccag ccgcgaccag gtgtgcctca tccccttcta cggcgacaag
     1741 gccgaggtgc tgctgccgcc ctccaagtcc atcgccatgg cccgccgccg cctggactcg
     1801 ctgccctgcg gcggcggctc gccccttgcg cacggcctgt ccacggcggt acgtgtgggc
     1861 atgcaggcca gccaggcggg cgaggtgggc cgcgtcatga tggtgctcat cacggacggc
     1921 cgcgccaacg tcagcctggc caagtccaac gaggaccccg aggcgctcaa gcccgacgcg
     1981 cccaagccca ccgccgactc gctgaaggac gaggtgcgcg acatggccaa gaaggccgcg
     2041 tccgccggca tcaacgtgct tgtcattgac acggagaaca agttcgtgag caccggcttt
     2101 gcggaggaga tctccaaggc agcgcagggc aagtactact acctgcccaa cgccagcgac
     2161 gcagcaatcg cagcagcagc atccggtgca atggcagcag ctaagggagg atactagcaa
     2221 taa
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