LOCUS       BBa_K1084124             472 bp    DNA     linear       27-May-2024
DEFINITION  pTet SD8 LacZα dT
ACCESSION   BBa_K1084124
VERSION     BBa_K1084124.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    63..81
                     /label=Enhancer sequence
     misc_feature    88..95
                     /label=SD sequence
     RBS             63..95
                     /label=BBa_K1084104
     CDS             102..335
                     /label=lacZ
     misc_feature    102..104
                     /label=START
     misc_feature    330..335
                     /label=STOP
     CDS             102..335
                     /label=BBa_I732006
     stem_loop       355..398
                     /label=stem_loop
     misc_feature    344..423
                     /label=BBa_B0010
     terminator      344..423
                     /label=BBa_B0010
     stem_loop       439..458
                     /label=T7 TE
     misc_feature    465..465
                     /label=stop
     polyA_site      459..472
                     /label=polya
     misc_feature    432..472
                     /label=BBa_B0012
     terminator      432..472
                     /label=BBa_B0012
     terminator      344..472
                     /label=BBa_B0015
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 aggctcttta acaatttatc agatccataa ggaggtacta gatgaccatg attacggatt
      121 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
      181 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
      241 gcccttccca acagttgcgc agcctgaatg gcgaatggcg ctttgcctgg tttccggcac
      301 cagaagcggt gccggaaagc tggctggagt aataatacta gagccaggca tcaaataaaa
      361 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct
      421 ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta ta
//