LOCUS       BBa_K1087022            1463 bp    DNA     linear       02-Jun-2024
DEFINITION  Lock+phiR73 activator+PO promoter+mRFP1
ACCESSION   BBa_K1087022
VERSION     BBa_K1087022.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    63..105
                     /label=BBa_J23032
     promoter        1..105
                     /label=BBa_K1087004
     misc_feature    112..494
                     /label=BBa_K1087006
     promoter        1..494
                     /label=BBa_K1087007
     promoter        503..594
                     /label=PO
     promoter        503..594
                     /label=BBa_I746361
     misc_feature    607..610
                     /label=conserved
     RBS             603..614
                     /label=BBa_B0034
     CDS             621..1295
                     /label=mrfp1
     misc_feature    1302..1326
                     /label=Help:Barcodes
     CDS             621..1301
                     /label=BBa_E1010
     stem_loop       1346..1389
                     /label=stem_loop
     misc_feature    1335..1414
                     /label=BBa_B0010
     terminator      1335..1414
                     /label=BBa_B0010
     stem_loop       1430..1449
                     /label=T7 TE
     misc_feature    1456..1456
                     /label=stop
     polyA_site      1450..1463
                     /label=polya
     misc_feature    1423..1463
                     /label=BBa_B0012
     terminator      1423..1463
                     /label=BBa_B0012
     misc_feature    603..1463
                     /label=BBa_I13507
     misc_feature    503..1463
                     /label=BBa_K1087018
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaactagaa tcacctcttg cttttgggta agacagaaga ggagatacta gatgatgcgc
      121 tgccctttct gtcgtcattc agcgcatacc cgcaccagcc ggtatgtgag tgacaatgtc
      181 aaagaaagtt atctccagtg ccagaatatt tactgttcgg cgacatttaa aacgcatgag
      241 tcaatttgtg ccgtgattcg ttctccggtc acggaggaaa aaccagcacc ggcaagcaca
      301 gcaccggctg ttgtccgaaa agttaaaggc tgttacagct caccattcaa ccattaatac
      361 tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat
      421 ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc
      481 tttctgcgtt tatatactag agcgcgcccc gcgattcgct aaggtgctgt tgtgtcagtg
      541 ataagccatc cgggactgat ggcggaggat gcgcatcgtc gggaaactga tgcctactag
      601 agaaagagga gaaatactag atggcttcct ccgaagacgt tatcaaagag ttcatgcgtt
      661 tcaaagttcg tatggaaggt tccgttaacg gtcacgagtt cgaaatcgaa ggtgaaggtg
      721 aaggtcgtcc gtacgaaggt acccagaccg ctaaactgaa agttaccaaa ggtggtccgc
      781 tgccgttcgc ttgggacatc ctgtccccgc agttccagta cggttccaaa gcttacgtta
      841 aacacccggc tgacatcccg gactacctga aactgtcctt cccggaaggt ttcaaatggg
      901 aacgtgttat gaacttcgaa gacggtggtg ttgttaccgt tacccaggac tcctccctgc
      961 aagacggtga gttcatctac aaagttaaac tgcgtggtac caacttcccg tccgacggtc
     1021 cggttatgca gaaaaaaacc atgggttggg aagcttccac cgaacgtatg tacccggaag
     1081 acggtgctct gaaaggtgaa atcaaaatgc gtctgaaact gaaagacggt ggtcactacg
     1141 acgctgaagt taaaaccacc tacatggcta aaaaaccggt tcagctgccg ggtgcttaca
     1201 aaaccgacat caaactggac atcacctccc acaacgaaga ctacaccatc gttgaacagt
     1261 acgaacgtgc tgaaggtcgt cactccaccg gtgcttaata acgctgatag tgctagtgta
     1321 gatcgctact agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt
     1381 tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc
     1441 gggtgggcct ttctgcgttt ata
//