Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Andreas Kj??r
Date created: 2013-07-08 11:00:00
Date modified: 2015-05-08 01:09:06
1-deoxyxylulose-5-phosphate synthase (Dxs) enzyme from MEP pathway isolated from B. subtilis 168
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K1088000_sequence (Version 1) |
Description
The MEP pathway is a common bacterial isoprenoid pathway. To improve the yield of the end product, isopentenyl diphosphate (IPP) and dimethylallyl pyrophosphate(DMAPP), Zhao et al. expressed, in 2011, the 1-deoxyxylulose-5-phosphate synthase(Dxs)gene aswell as the second step of the MEP pathway. They saw a 2.3 fold increase in isoprene output (isoprene is one of many products from the MEP pathway).From Biocyc:
"Dxs catalyzes the thiamin diphosphate-dependent reaction that condenses pyruvate and D-glyceraldehyde-3-phosphate to yield 1-deoxy-D-xylulose 5-phosphate [Julsing07]. This is the first, rate-limiting step in the methylerythritol phosphate pathway of isoprenoid biosynthesis, and is believed to feed into the pyridoxal 5-phosphate (vitamin B6) and thiamin (vitamin B1) biosynthesis pathways."
-http://biocyc.org/BSUB/NEW-IMAGE?type=ENZYME&object=BSU24270-MONOMER
Notes
we changed TTG start codon to ATG to improve translation efficiency.We performed 4 silent site directed mutageneses to remove 2 EcoR1 and 2 Pst1 restriction sites
Source
B. subtilis 168| Sequence Annotation | Location | Component / Role(s) |
| Dxs B. sub coding region | 1,1901 | feature/cds CDS |