LOCUS       BBa_K1088007            2832 bp    DNA     linear       17-May-2024
DEFINITION  E. coli dxs-GFP fusion (lac promoter without lac inhibitor: IPTG 
            uninducible)
ACCESSION   BBa_K1088007
VERSION     BBa_K1088007.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    201..206
                     /label=BBa_K1088021
     misc_feature    207..209
                     /label=SacI
     RBS             210..216
                     /label=rbs
     RBS             207..216
                     /label=BBa_J15001
     CDS             217..2079
                     /label=dxs coding sequence
     CDS             217..2082
                     /label=BBa_K118000
     misc_feature    2083..2112
                     /label=BBa_K105012
     CDS             2113..2832
                     /label=GFP protein
     CDS             2113..2832
                     /label=BBa_E0040
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca actagactca aggaggatga gttttgatat tgccaaatac
      241 ccgaccctgg cactggtcga ctccacccag gagttacgac tgttgccgaa agagagttta
      301 ccgaaactct gcgacgaact gcgccgctat ttactcgaca gcgtgagccg ttccagcggg
      361 cacttcgcct ccgggctggg cacggtcgaa ctgaccgtgg cgctgcacta tgtctacaac
      421 accccgtttg accaattgat ttgggatgtg gggcatcagg cttatccgca taaaattttg
      481 accggacgcc gcgacaaaat cggcaccatc cgtcagaaag gcggtctgca cccgttcccg
      541 tggcgcggcg aaagcgaata tgacgtatta agcgtcgggc attcatcaac ctccatcagt
      601 gccggaattg gtattgcggt tgctgccgaa aaagaaggca aaaatcgccg caccgtctgt
      661 gtcattggcg atggcgcgat taccgcaggc atggcgtttg aagcgatgaa tcacgcgggc
      721 gatatccgtc ctgatatgct ggtgattctc aacgacaatg aaatgtcgat ttccgaaaat
      781 gtcggcgcgc tcaacaacca tctggcacag ctgctttccg gtaagcttta ctcttcactg
      841 cgcgaaggcg ggaaaaaagt tttctctggc gtgccgccaa ttaaagagct gctcaaacgc
      901 accgaagaac atattaaagg catggtagtg cctggcacgt tgtttgaaga gctgggcttt
      961 aactacatcg gcccggtgga cggtcacgat gtgctggggc ttatcaccac gctaaagaac
     1021 atgcgcgacc tgaaaggccc gcagttcctg catatcatga ccaaaaaagg tcgtggttat
     1081 gaaccggcag aaaaagaccc gatcactttc cacgccgtgc ctaaatttga tccctccagc
     1141 ggttgtttgc cgaaaagtag cggcggtttg ccgagctatt caaaaatctt tggcgactgg
     1201 ttgtgcgaaa cggcagcgaa agacaacaag ctgatggcga ttactccggc gatgcgtgaa
     1261 ggttccggca tggtcgagtt ttcacgtaaa ttcccggatc gctacttcga cgtggcaatt
     1321 gccgagcaac acgcggtgac ctttgctgcg ggtctggcga ttggtgggta caaacccatt
     1381 gtcgcgattt actccacttt cctgcaacgc gcctatgatc aggtgctgca tgacgtggcg
     1441 attcaaaagc ttccggtcct gttcgccatc gaccgcgcgg gcattgttgg tgctgacggt
     1501 caaacccatc agggtgcttt tgatctctct tacctgcgct gcataccgga aatggtcatt
     1561 atgaccccga gcgatgaaaa cgaatgtcgc cagatgctct ataccggcta tcactataac
     1621 gatggcccgt cagcggtgcg ctacccgcgt ggcaacgcgg tcggcgtgga actgacgccg
     1681 ctggaaaaac taccaattgg caaaggcatt gtgaagcgtc gtggcgagaa actggcgatc
     1741 cttaactttg gtacgctgat gccagaagcg gcgaaagtcg ccgaatcgct gaacgccacg
     1801 ctggtcgata tgcgttttgt gaaaccgctt gatgaagcgt taattctgga aatggccgcc
     1861 agccatgaag cgctggtcac cgtagaagaa aacgccatta tgggcggcgc aggcagcggc
     1921 gtgaacgaag tgctgatggc ccatcgtaaa ccagtacccg tgctgaacat tggcctgccg
     1981 gacttcttta ttccgcaagg aactcaggaa gaaatgcgcg ccgaactcgg cctcgatgcc
     2041 gctggtatgg aagccaaaat caaggcctgg ctggcataat aaggtgaaaa tttgtatttt
     2101 caatctggtg gtatgcgtaa aggagaagaa cttttcactg gagttgtccc aattcttgtt
     2161 gaattagatg gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat
     2221 gcaacatacg gaaaacttac ccttaaattt atttgcacta ctggaaaact acctgttcca
     2281 tggccaacac ttgtcactac tttcggttat ggtgttcaat gctttgcgag atacccagat
     2341 catatgaaac agcatgactt tttcaagagt gccatgcccg aaggttatgt acaggaaaga
     2401 actatatttt tcaaagatga cgggaactac aagacacgtg ctgaagtcaa gtttgaaggt
     2461 gatacccttg ttaatagaat cgagttaaaa ggtattgatt ttaaagaaga tggaaacatt
     2521 cttggacaca aattggaata caactataac tcacacaatg tatacatcat ggcagacaaa
     2581 caaaagaatg gaatcaaagt taacttcaaa attagacaca acattgaaga tggaagcgtt
     2641 caactagcag accattatca acaaaatact ccaattggcg atggccctgt ccttttacca
     2701 gacaaccatt acctgtccac acaatctgcc ctttcgaaag atcccaacga aaagagagac
     2761 cacatggtcc ttcttgagtt tgtaacagct gctgggatta cacatggcat ggatgaacta
     2821 tacaaataat aa
//