LOCUS       BBa_K1088008            2868 bp    DNA     linear       18-May-2024
DEFINITION  B. subtilis dxs-GFP fusion (lac promoter without lac inhibitor: 
            IPTG uninducible)
ACCESSION   BBa_K1088008
VERSION     BBa_K1088008.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    201..206
                     /label=BBa_K1088021
     misc_feature    207..209
                     /label=SacI
     RBS             210..216
                     /label=rbs
     RBS             207..216
                     /label=BBa_J15001
     CDS             217..2117
                     /label=Dxs B. sub coding region
     CDS             217..2118
                     /label=BBa_K1088000
     misc_feature    2119..2148
                     /label=BBa_K105012
     CDS             2149..2868
                     /label=GFP protein
     CDS             2149..2868
                     /label=BBa_E0040
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca actagactca aggaggatgg atcttttatc aatacaggac
      241 ccgtcgtttt taaaaaacat gtccattgat gaattagaga aattaagtga tgaaatccgt
      301 cagtttttaa ttacaagttt atccgcttcc ggcggccaca tcggcccaaa cttaggtgtc
      361 gtagagctta ctgttgccct gcataaggaa tttaacagcc cgaaagacaa atttttatgg
      421 gatgtaggcc atcagtcgta tgtccataag ctgctgacag gacgcggaaa agaatttgcg
      481 acgcttcgcc agtacaaagg gctttgcgga tttccaaagc ggagtgaaag cgagcacgat
      541 gtttgggaaa ccgggcacag ctcgacttct ctgtcaggcg cgatgggaat ggcagctgcc
      601 cgtgatatta aaggaacgga tgaatatatt attccgatca ttggtgacgg cgcgctgacc
      661 ggcggtatgg cgctcgaagc ccttaaccac atcggcgacg agaaaaaaga catgattgtc
      721 atccttaatg ataatgaaat gagtattgcg ccaaacgtcg gtgccattca ctctatgctc
      781 ggacggctcc gcactgcggg gaaataccag tgggtcaaag atgagcttga atacttattt
      841 aaaaagattc cggcagttgg gggcaagctt gccgccacgg cggaacgggt caaagacagc
      901 ctgaaataca tgctcgtctc cggaatgttt ttcgaggagc tcggttttac gtatttgggc
      961 ccagtggacg gacattctta tcatgagctg attgagaatc ttcaatacgc caaaaaaacg
     1021 aaaggccctg ttcttctgca cgtcattacg aaaaaaggga aggggtacaa accggctgag
     1081 accgatacga ttgggacatg gcatggtacc ggaccatata aaattaatac cggtgacttt
     1141 gtaaagccga aagccgcagc tccttcgtgg agcggtcttg tcagcggaac tgtgcagcga
     1201 atggcgcgcg aggacggacg cattgtagcc attacgccgg ctatgcctgt cggttcaaag
     1261 cttgaaggct tcgcaaagga attccctgac cggatgttcg acgtaggaat cgcagaacag
     1321 catgccgcaa caatggctgc agctatggca atgcagggta tgaagccgtt tttggcgatt
     1381 tactcaacct tcctgcaaag ggcatatgac caagttgttc atgacatctg ccgccaaaac
     1441 gctaatgtgt ttattggaat tgaccgtgct ggactcgttg gcgctgatgg agagacacat
     1501 caaggcgtgt ttgatattgc gtttatgcgc cacattccaa acatggtctt aatgatgccg
     1561 aaagacgaaa atgaaggcca gcacatggtt catacagcac ttagctatga cgaaggcccg
     1621 atagcaatgc gttttccgcg cggaaacgga ctcggcgtaa aaatggatga acagttgaaa
     1681 acgattccga tcggtacgtg ggaggtgctg cgtccaggga acgatgctgt catcttaaca
     1741 ttcggcacaa caatcgaaat ggcgattgaa gcagccgaag agctgcagaa agaaggcctt
     1801 tccgtgcgcg ttgtgaatgc gcgttttatt aagccgattg atgaaaagat gatgaagagt
     1861 atcctaaaag aaggcttgcc aattttaaca attgaagaag cggtcttaga aggcggtttc
     1921 ggaagctcga ttttagaatt cgctcatgat caaggtgaat atcatactcc gattgacaga
     1981 atgggtatac ctgatcggtt tattgaacac ggaagtgtaa cagcgcttct tgaggaaatt
     2041 ggactgacaa aacagcaggt ggcaaatcgt attagattac tgatgccacc aaagacacac
     2101 aaaggaattg gatcatgagg tgaaaatttg tattttcaat ctggtggtat gcgtaaagga
     2161 gaagaacttt tcactggagt tgtcccaatt cttgttgaat tagatggtga tgttaatggg
     2221 cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa acttaccctt
     2281 aaatttattt gcactactgg aaaactacct gttccatggc caacacttgt cactactttc
     2341 ggttatggtg ttcaatgctt tgcgagatac ccagatcata tgaaacagca tgactttttc
     2401 aagagtgcca tgcccgaagg ttatgtacag gaaagaacta tatttttcaa agatgacggg
     2461 aactacaaga cacgtgctga agtcaagttt gaaggtgata cccttgttaa tagaatcgag
     2521 ttaaaaggta ttgattttaa agaagatgga aacattcttg gacacaaatt ggaatacaac
     2581 tataactcac acaatgtata catcatggca gacaaacaaa agaatggaat caaagttaac
     2641 ttcaaaatta gacacaacat tgaagatgga agcgttcaac tagcagacca ttatcaacaa
     2701 aatactccaa ttggcgatgg ccctgtcctt ttaccagaca accattacct gtccacacaa
     2761 tctgcccttt cgaaagatcc caacgaaaag agagaccaca tggtccttct tgagtttgta
     2821 acagctgctg ggattacaca tggcatggat gaactataca aataataa
//