LOCUS BBa_K1088009 4094 bp DNA linear 13-May-2024 DEFINITION B. subtilis dxs-GFP protein fusion (lac promoter with LVA-tagged lac inhibitor (LacI:LVA) - IPTG ind ACCESSION BBa_K1088009 VERSION BBa_K1088009.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23106 RBS 36..50 /label=RBS-1\Strong RBS 43..47 /label=RBS misc_feature 36..50 /label=BBa_B0030 RBS 36..50 /label=BBa_B0030 misc_feature 51..56 /label=BBa_K1088022 CDS 57..1184 /label=LacI-LVA misc_feature 1146..1184 /label=LVA CDS 57..1184 /label=BBa_K1088023 misc_feature 1185..1218 /label=B1002 stem_loop 1194..1209 /label=stem loop polyA_site 1209..1215 /label=Poly A tail polyA_site 1188..1193 /label=Poly A tail terminator 1185..1218 /label=BBa_B1002 promoter 1..1218 /label=BBa_K1088020 CDS 1227..1314 /label=end of LacI coding region (inactive) misc_feature 1227..1426 /label=BBa_R0010 misc_feature 1315..1352 /label=CAP binding site promoter 1363..1368 /label=-35 promoter 1387..1392 /label=-10 misc_feature 1392..1426 /label=LacI binding site misc_feature 1399..1399 /label=start promoter 1227..1426 /label=BBa_R0010 misc_feature 1427..1432 /label=BBa_K1088021 misc_feature 1433..1435 /label=SacI RBS 1436..1442 /label=rbs RBS 1433..1442 /label=BBa_J15001 CDS 1443..3343 /label=Dxs B. sub coding region CDS 1443..3344 /label=BBa_K1088000 misc_feature 3345..3374 /label=BBa_K105012 CDS 3375..4094 /label=GFP protein CDS 3375..4094 /label=BBa_E0040 misc_feature 1227..4094 /label=BBa_K1088008 ORIGIN 1 tttacggcta gctcagtcct aggtatagtg ctagcattaa agaggagaaa tactagatgg 61 tgaatgtgaa accagtaacg ttatacgatg tcgcagagta tgccggtgtc tcttatcaga 121 ccgtttcccg cgtggtgaac caggccagcc acgtttctgc gaaaacgcgg gaaaaagtgg 181 aagcggcgat ggcggagctg aattacattc ccaaccgcgt ggcacaacaa ctggcgggca 241 aacagtcgtt gctgattggc gttgccacct ccagtctggc cctgcacgcg ccgtcgcaaa 301 ttgtcgcggc gattaaatct cgcgccgatc aactgggtgc cagcgtggtg gtgtcgatgg 361 tagaacgaag cggcgtcgaa gcctgtaaag cggcggtgca caatcttctc gcgcaacgcg 421 tcagtgggct gatcattaac tatccgctgg atgaccagga tgccattgct gtggaagctg 481 cctgcactaa tgttccggcg ttatttcttg atgtctctga ccagacaccc atcaacagta 541 ttattttctc ccatgaagac ggtacgcgac tgggcgtgga gcatctggtc gcattgggtc 601 accagcaaat cgcgctgtta gcgggcccat taagttctgt ctcggcgcgt ctgcgtctgg 661 ctggctggca taaatatctc actcgcaatc aaattcagcc gatagcggaa cgggaaggcg 721 actggagtgc catgtccggt tttcaacaaa ccatgcaaat gctgaatgag ggcatcgttc 781 ccactgcgat gctggttgcc aacgatcaga tggcgctggg cgcaatgcgc gccattaccg 841 agtccgggct gcgcgttggt gcggatatct cggtagtggg atacgacgat accgaagaca 901 gctcatgtta tatcccgccg ttaaccacca tcaaacagga ttttcgcctg ctggggcaaa 961 ccagcgtgga ccgcttgctg caactctctc agggccaggc ggtgaagggc aatcagctgt 1021 tgcccgtctc actggtgaaa agaaaaacca ccctggcgcc caatacgcaa accgcctctc 1081 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg 1141 ggcaggctgc aaacgacgaa aactacgctt tagtagctta ataacgcaaa aaaccccgct 1201 tcggcggggt tttttcgcta ctagagcaat acgcaaaccg cctctccccg cgcgttggcc 1261 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa 1321 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc 1381 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaacta gactcaagga 1441 ggatggatct tttatcaata caggacccgt cgtttttaaa aaacatgtcc attgatgaat 1501 tagagaaatt aagtgatgaa atccgtcagt ttttaattac aagtttatcc gcttccggcg 1561 gccacatcgg cccaaactta ggtgtcgtag agcttactgt tgccctgcat aaggaattta 1621 acagcccgaa agacaaattt ttatgggatg taggccatca gtcgtatgtc cataagctgc 1681 tgacaggacg cggaaaagaa tttgcgacgc ttcgccagta caaagggctt tgcggatttc 1741 caaagcggag tgaaagcgag cacgatgttt gggaaaccgg gcacagctcg acttctctgt 1801 caggcgcgat gggaatggca gctgcccgtg atattaaagg aacggatgaa tatattattc 1861 cgatcattgg tgacggcgcg ctgaccggcg gtatggcgct cgaagccctt aaccacatcg 1921 gcgacgagaa aaaagacatg attgtcatcc ttaatgataa tgaaatgagt attgcgccaa 1981 acgtcggtgc cattcactct atgctcggac ggctccgcac tgcggggaaa taccagtggg 2041 tcaaagatga gcttgaatac ttatttaaaa agattccggc agttgggggc aagcttgccg 2101 ccacggcgga acgggtcaaa gacagcctga aatacatgct cgtctccgga atgtttttcg 2161 aggagctcgg ttttacgtat ttgggcccag tggacggaca ttcttatcat gagctgattg 2221 agaatcttca atacgccaaa aaaacgaaag gccctgttct tctgcacgtc attacgaaaa 2281 aagggaaggg gtacaaaccg gctgagaccg atacgattgg gacatggcat ggtaccggac 2341 catataaaat taataccggt gactttgtaa agccgaaagc cgcagctcct tcgtggagcg 2401 gtcttgtcag cggaactgtg cagcgaatgg cgcgcgagga cggacgcatt gtagccatta 2461 cgccggctat gcctgtcggt tcaaagcttg aaggcttcgc aaaggaattc cctgaccgga 2521 tgttcgacgt aggaatcgca gaacagcatg ccgcaacaat ggctgcagct atggcaatgc 2581 agggtatgaa gccgtttttg gcgatttact caaccttcct gcaaagggca tatgaccaag 2641 ttgttcatga catctgccgc caaaacgcta atgtgtttat tggaattgac cgtgctggac 2701 tcgttggcgc tgatggagag acacatcaag gcgtgtttga tattgcgttt atgcgccaca 2761 ttccaaacat ggtcttaatg atgccgaaag acgaaaatga aggccagcac atggttcata 2821 cagcacttag ctatgacgaa ggcccgatag caatgcgttt tccgcgcgga aacggactcg 2881 gcgtaaaaat ggatgaacag ttgaaaacga ttccgatcgg tacgtgggag gtgctgcgtc 2941 cagggaacga tgctgtcatc ttaacattcg gcacaacaat cgaaatggcg attgaagcag 3001 ccgaagagct gcagaaagaa ggcctttccg tgcgcgttgt gaatgcgcgt tttattaagc 3061 cgattgatga aaagatgatg aagagtatcc taaaagaagg cttgccaatt ttaacaattg 3121 aagaagcggt cttagaaggc ggtttcggaa gctcgatttt agaattcgct catgatcaag 3181 gtgaatatca tactccgatt gacagaatgg gtatacctga tcggtttatt gaacacggaa 3241 gtgtaacagc gcttcttgag gaaattggac tgacaaaaca gcaggtggca aatcgtatta 3301 gattactgat gccaccaaag acacacaaag gaattggatc atgaggtgaa aatttgtatt 3361 ttcaatctgg tggtatgcgt aaaggagaag aacttttcac tggagttgtc ccaattcttg 3421 ttgaattaga tggtgatgtt aatgggcaca aattttctgt cagtggagag ggtgaaggtg 3481 atgcaacata cggaaaactt acccttaaat ttatttgcac tactggaaaa ctacctgttc 3541 catggccaac acttgtcact actttcggtt atggtgttca atgctttgcg agatacccag 3601 atcatatgaa acagcatgac tttttcaaga gtgccatgcc cgaaggttat gtacaggaaa 3661 gaactatatt tttcaaagat gacgggaact acaagacacg tgctgaagtc aagtttgaag 3721 gtgataccct tgttaataga atcgagttaa aaggtattga ttttaaagaa gatggaaaca 3781 ttcttggaca caaattggaa tacaactata actcacacaa tgtatacatc atggcagaca 3841 aacaaaagaa tggaatcaaa gttaacttca aaattagaca caacattgaa gatggaagcg 3901 ttcaactagc agaccattat caacaaaata ctccaattgg cgatggccct gtccttttac 3961 cagacaacca ttacctgtcc acacaatctg ccctttcgaa agatcccaac gaaaagagag 4021 accacatggt ccttcttgag tttgtaacag ctgctgggat tacacatggc atggatgaac 4081 tatacaaata ataa //