LOCUS BBa_K1088010 4013 bp DNA linear 11-May-2024 DEFINITION E. coli dxs-GFP protein fusion (lac promoter with LVA-tagged lac inhibitor (LacI:LVA) - IPTG inducib ACCESSION BBa_K1088010 VERSION BBa_K1088010.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23106 RBS 36..50 /label=RBS-1\Strong RBS 43..47 /label=RBS misc_feature 36..50 /label=BBa_B0030 RBS 36..50 /label=BBa_B0030 misc_feature 51..56 /label=BBa_K1088022 CDS 57..1139 /label=BBa_K1088018 misc_feature 1140..1173 /label=B1002 stem_loop 1149..1164 /label=stem loop polyA_site 1164..1170 /label=Poly A tail polyA_site 1143..1148 /label=Poly A tail terminator 1140..1173 /label=BBa_B1002 promoter 1..1173 /label=BBa_K1088019 CDS 1182..1269 /label=end of LacI coding region (inactive) misc_feature 1182..1381 /label=BBa_R0010 misc_feature 1270..1307 /label=CAP binding site promoter 1318..1323 /label=-35 promoter 1342..1347 /label=-10 misc_feature 1347..1381 /label=LacI binding site misc_feature 1354..1354 /label=start promoter 1182..1381 /label=BBa_R0010 misc_feature 1382..1387 /label=BBa_K1088021 misc_feature 1388..1390 /label=SacI RBS 1391..1397 /label=rbs RBS 1388..1397 /label=BBa_J15001 CDS 1398..3260 /label=dxs coding sequence CDS 1398..3263 /label=BBa_K118000 misc_feature 3264..3293 /label=BBa_K105012 CDS 3294..4013 /label=GFP protein CDS 3294..4013 /label=BBa_E0040 misc_feature 1182..4013 /label=BBa_K1088007 ORIGIN 1 tttacggcta gctcagtcct aggtatagtg ctagcattaa agaggagaaa tactagatga 61 aaccagtaac gttatacgat gtcgcagagt atgccggtgt ctcttatcag accgtttccc 121 gcgtggtgaa ccaggccagc cacgtttctg cgaaaacgcg ggaaaaagtg gaagcggcga 181 tggcggagct gaattacatt cccaaccgcg tggcacaaca actggcgggc aaacagtcgt 241 tgctgattgg cgttgccacc tccagtctgg ccctgcacgc gccgtcgcaa attgtcgcgg 301 cgattaaatc tcgcgccgat caactgggtg ccagcgtggt ggtgtcgatg gtagaacgaa 361 gcggcgtcga agcctgtaaa gcggcggtgc acaatcttct cgcgcaacgc gtcagtgggc 421 tgatcattaa ctatccgctg gatgaccagg atgccattgc tgtggaagct gcctgcacta 481 atgttccggc gttatttctt gatgtctctg accagacacc catcaacagt attattttct 541 cccatgaaga cggtacgcga ctgggcgtgg agcatctggt cgcattgggt caccagcaaa 601 tcgcgctgtt agcgggccca ttaagttctg tctcggcgcg tctgcgtctg gctggctggc 661 ataaatatct cactcgcaat caaattcagc cgatagcgga acgggaaggc gactggagtg 721 ccatgtccgg ttttcaacaa accatgcaaa tgctgaatga gggcatcgtt cccactgcga 781 tgctggttgc caacgatcag atggcgctgg gcgcaatgcg cgccattacc gagtccgggc 841 tgcgcgttgg tgcggatatc tcggtagtgg gatacgacga taccgaagac agctcatgtt 901 atatcccgcc gttaaccacc atcaaacagg attttcgcct gctggggcaa accagcgtgg 961 accgcttgct gcaactctct cagggccagg cggtgaaggg caatcagctg ttgcccgtct 1021 cactggtgaa aagaaaaacc accctggcgc ccaatacgca aaccgcctct ccccgcgcgt 1081 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgac 1141 gcaaaaaacc ccgcttcggc ggggtttttt cgctactaga gcaatacgca aaccgcctct 1201 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc 1261 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt 1321 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac 1381 aactagactc aaggaggatg agttttgata ttgccaaata cccgaccctg gcactggtcg 1441 actccaccca ggagttacga ctgttgccga aagagagttt accgaaactc tgcgacgaac 1501 tgcgccgcta tttactcgac agcgtgagcc gttccagcgg gcacttcgcc tccgggctgg 1561 gcacggtcga actgaccgtg gcgctgcact atgtctacaa caccccgttt gaccaattga 1621 tttgggatgt ggggcatcag gcttatccgc ataaaatttt gaccggacgc cgcgacaaaa 1681 tcggcaccat ccgtcagaaa ggcggtctgc acccgttccc gtggcgcggc gaaagcgaat 1741 atgacgtatt aagcgtcggg cattcatcaa cctccatcag tgccggaatt ggtattgcgg 1801 ttgctgccga aaaagaaggc aaaaatcgcc gcaccgtctg tgtcattggc gatggcgcga 1861 ttaccgcagg catggcgttt gaagcgatga atcacgcggg cgatatccgt cctgatatgc 1921 tggtgattct caacgacaat gaaatgtcga tttccgaaaa tgtcggcgcg ctcaacaacc 1981 atctggcaca gctgctttcc ggtaagcttt actcttcact gcgcgaaggc gggaaaaaag 2041 ttttctctgg cgtgccgcca attaaagagc tgctcaaacg caccgaagaa catattaaag 2101 gcatggtagt gcctggcacg ttgtttgaag agctgggctt taactacatc ggcccggtgg 2161 acggtcacga tgtgctgggg cttatcacca cgctaaagaa catgcgcgac ctgaaaggcc 2221 cgcagttcct gcatatcatg accaaaaaag gtcgtggtta tgaaccggca gaaaaagacc 2281 cgatcacttt ccacgccgtg cctaaatttg atccctccag cggttgtttg ccgaaaagta 2341 gcggcggttt gccgagctat tcaaaaatct ttggcgactg gttgtgcgaa acggcagcga 2401 aagacaacaa gctgatggcg attactccgg cgatgcgtga aggttccggc atggtcgagt 2461 tttcacgtaa attcccggat cgctacttcg acgtggcaat tgccgagcaa cacgcggtga 2521 cctttgctgc gggtctggcg attggtgggt acaaacccat tgtcgcgatt tactccactt 2581 tcctgcaacg cgcctatgat caggtgctgc atgacgtggc gattcaaaag cttccggtcc 2641 tgttcgccat cgaccgcgcg ggcattgttg gtgctgacgg tcaaacccat cagggtgctt 2701 ttgatctctc ttacctgcgc tgcataccgg aaatggtcat tatgaccccg agcgatgaaa 2761 acgaatgtcg ccagatgctc tataccggct atcactataa cgatggcccg tcagcggtgc 2821 gctacccgcg tggcaacgcg gtcggcgtgg aactgacgcc gctggaaaaa ctaccaattg 2881 gcaaaggcat tgtgaagcgt cgtggcgaga aactggcgat ccttaacttt ggtacgctga 2941 tgccagaagc ggcgaaagtc gccgaatcgc tgaacgccac gctggtcgat atgcgttttg 3001 tgaaaccgct tgatgaagcg ttaattctgg aaatggccgc cagccatgaa gcgctggtca 3061 ccgtagaaga aaacgccatt atgggcggcg caggcagcgg cgtgaacgaa gtgctgatgg 3121 cccatcgtaa accagtaccc gtgctgaaca ttggcctgcc ggacttcttt attccgcaag 3181 gaactcagga agaaatgcgc gccgaactcg gcctcgatgc cgctggtatg gaagccaaaa 3241 tcaaggcctg gctggcataa taaggtgaaa atttgtattt tcaatctggt ggtatgcgta 3301 aaggagaaga acttttcact ggagttgtcc caattcttgt tgaattagat ggtgatgtta 3361 atgggcacaa attttctgtc agtggagagg gtgaaggtga tgcaacatac ggaaaactta 3421 cccttaaatt tatttgcact actggaaaac tacctgttcc atggccaaca cttgtcacta 3481 ctttcggtta tggtgttcaa tgctttgcga gatacccaga tcatatgaaa cagcatgact 3541 ttttcaagag tgccatgccc gaaggttatg tacaggaaag aactatattt ttcaaagatg 3601 acgggaacta caagacacgt gctgaagtca agtttgaagg tgataccctt gttaatagaa 3661 tcgagttaaa aggtattgat tttaaagaag atggaaacat tcttggacac aaattggaat 3721 acaactataa ctcacacaat gtatacatca tggcagacaa acaaaagaat ggaatcaaag 3781 ttaacttcaa aattagacac aacattgaag atggaagcgt tcaactagca gaccattatc 3841 aacaaaatac tccaattggc gatggccctg tccttttacc agacaaccat tacctgtcca 3901 cacaatctgc cctttcgaaa gatcccaacg aaaagagaga ccacatggtc cttcttgagt 3961 ttgtaacagc tgctgggatt acacatggca tggatgaact atacaaataa taa //