LOCUS       BBa_K1088014            3294 bp    DNA     linear       17-May-2024
DEFINITION  E. coli dxs (lac promoter with lac inhibitor: IPTG inducible)
ACCESSION   BBa_K1088014
VERSION     BBa_K1088014.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23106
     RBS             36..50
                     /label=RBS-1\Strong
     RBS             43..47
                     /label=RBS
     misc_feature    36..50
                     /label=BBa_B0030
     RBS             36..50
                     /label=BBa_B0030
     misc_feature    51..56
                     /label=BBa_K1088022
     CDS             57..1184
                     /label=LacI-LVA
     misc_feature    1146..1184
                     /label=LVA
     CDS             57..1184
                     /label=BBa_K1088023
     misc_feature    1185..1218
                     /label=B1002
     stem_loop       1194..1209
                     /label=stem loop
     polyA_site      1209..1215
                     /label=Poly A tail
     polyA_site      1188..1193
                     /label=Poly A tail
     terminator      1185..1218
                     /label=BBa_B1002
     CDS             1219..1306
                     /label=end of LacI coding region (inactive)
     misc_feature    1219..1418
                     /label=BBa_R0010
     misc_feature    1307..1344
                     /label=CAP binding site
     promoter        1355..1360
                     /label=-35
     promoter        1379..1384
                     /label=-10
     misc_feature    1384..1418
                     /label=LacI binding site
     misc_feature    1391..1391
                     /label=start
     promoter        1219..1418
                     /label=BBa_R0010
     misc_feature    1419..1421
                     /label=SacI
     RBS             1422..1428
                     /label=rbs
     RBS             1419..1428
                     /label=BBa_J15001
     CDS             1429..3291
                     /label=dxs coding sequence
     CDS             1429..3294
                     /label=BBa_K118000
ORIGIN
        1 tttacggcta gctcagtcct aggtatagtg ctagcattaa agaggagaaa tactagatgg
       61 tgaatgtgaa accagtaacg ttatacgatg tcgcagagta tgccggtgtc tcttatcaga
      121 ccgtttcccg cgtggtgaac caggccagcc acgtttctgc gaaaacgcgg gaaaaagtgg
      181 aagcggcgat ggcggagctg aattacattc ccaaccgcgt ggcacaacaa ctggcgggca
      241 aacagtcgtt gctgattggc gttgccacct ccagtctggc cctgcacgcg ccgtcgcaaa
      301 ttgtcgcggc gattaaatct cgcgccgatc aactgggtgc cagcgtggtg gtgtcgatgg
      361 tagaacgaag cggcgtcgaa gcctgtaaag cggcggtgca caatcttctc gcgcaacgcg
      421 tcagtgggct gatcattaac tatccgctgg atgaccagga tgccattgct gtggaagctg
      481 cctgcactaa tgttccggcg ttatttcttg atgtctctga ccagacaccc atcaacagta
      541 ttattttctc ccatgaagac ggtacgcgac tgggcgtgga gcatctggtc gcattgggtc
      601 accagcaaat cgcgctgtta gcgggcccat taagttctgt ctcggcgcgt ctgcgtctgg
      661 ctggctggca taaatatctc actcgcaatc aaattcagcc gatagcggaa cgggaaggcg
      721 actggagtgc catgtccggt tttcaacaaa ccatgcaaat gctgaatgag ggcatcgttc
      781 ccactgcgat gctggttgcc aacgatcaga tggcgctggg cgcaatgcgc gccattaccg
      841 agtccgggct gcgcgttggt gcggatatct cggtagtggg atacgacgat accgaagaca
      901 gctcatgtta tatcccgccg ttaaccacca tcaaacagga ttttcgcctg ctggggcaaa
      961 ccagcgtgga ccgcttgctg caactctctc agggccaggc ggtgaagggc aatcagctgt
     1021 tgcccgtctc actggtgaaa agaaaaacca ccctggcgcc caatacgcaa accgcctctc
     1081 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     1141 ggcaggctgc aaacgacgaa aactacgctt tagtagctta ataacgcaaa aaaccccgct
     1201 tcggcggggt tttttcgcca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     1261 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     1321 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     1381 tgttgtgtgg aattgtgagc ggataacaat ttcacacact caaggaggat gagttttgat
     1441 attgccaaat acccgaccct ggcactggtc gactccaccc aggagttacg actgttgccg
     1501 aaagagagtt taccgaaact ctgcgacgaa ctgcgccgct atttactcga cagcgtgagc
     1561 cgttccagcg ggcacttcgc ctccgggctg ggcacggtcg aactgaccgt ggcgctgcac
     1621 tatgtctaca acaccccgtt tgaccaattg atttgggatg tggggcatca ggcttatccg
     1681 cataaaattt tgaccggacg ccgcgacaaa atcggcacca tccgtcagaa aggcggtctg
     1741 cacccgttcc cgtggcgcgg cgaaagcgaa tatgacgtat taagcgtcgg gcattcatca
     1801 acctccatca gtgccggaat tggtattgcg gttgctgccg aaaaagaagg caaaaatcgc
     1861 cgcaccgtct gtgtcattgg cgatggcgcg attaccgcag gcatggcgtt tgaagcgatg
     1921 aatcacgcgg gcgatatccg tcctgatatg ctggtgattc tcaacgacaa tgaaatgtcg
     1981 atttccgaaa atgtcggcgc gctcaacaac catctggcac agctgctttc cggtaagctt
     2041 tactcttcac tgcgcgaagg cgggaaaaaa gttttctctg gcgtgccgcc aattaaagag
     2101 ctgctcaaac gcaccgaaga acatattaaa ggcatggtag tgcctggcac gttgtttgaa
     2161 gagctgggct ttaactacat cggcccggtg gacggtcacg atgtgctggg gcttatcacc
     2221 acgctaaaga acatgcgcga cctgaaaggc ccgcagttcc tgcatatcat gaccaaaaaa
     2281 ggtcgtggtt atgaaccggc agaaaaagac ccgatcactt tccacgccgt gcctaaattt
     2341 gatccctcca gcggttgttt gccgaaaagt agcggcggtt tgccgagcta ttcaaaaatc
     2401 tttggcgact ggttgtgcga aacggcagcg aaagacaaca agctgatggc gattactccg
     2461 gcgatgcgtg aaggttccgg catggtcgag ttttcacgta aattcccgga tcgctacttc
     2521 gacgtggcaa ttgccgagca acacgcggtg acctttgctg cgggtctggc gattggtggg
     2581 tacaaaccca ttgtcgcgat ttactccact ttcctgcaac gcgcctatga tcaggtgctg
     2641 catgacgtgg cgattcaaaa gcttccggtc ctgttcgcca tcgaccgcgc gggcattgtt
     2701 ggtgctgacg gtcaaaccca tcagggtgct tttgatctct cttacctgcg ctgcataccg
     2761 gaaatggtca ttatgacccc gagcgatgaa aacgaatgtc gccagatgct ctataccggc
     2821 tatcactata acgatggccc gtcagcggtg cgctacccgc gtggcaacgc ggtcggcgtg
     2881 gaactgacgc cgctggaaaa actaccaatt ggcaaaggca ttgtgaagcg tcgtggcgag
     2941 aaactggcga tccttaactt tggtacgctg atgccagaag cggcgaaagt cgccgaatcg
     3001 ctgaacgcca cgctggtcga tatgcgtttt gtgaaaccgc ttgatgaagc gttaattctg
     3061 gaaatggccg ccagccatga agcgctggtc accgtagaag aaaacgccat tatgggcggc
     3121 gcaggcagcg gcgtgaacga agtgctgatg gcccatcgta aaccagtacc cgtgctgaac
     3181 attggcctgc cggacttctt tattccgcaa ggaactcagg aagaaatgcg cgccgaactc
     3241 ggcctcgatg ccgctggtat ggaagccaaa atcaaggcct ggctggcata ataa
//