LOCUS       BBa_K1088020            1218 bp    DNA     linear       12-May-2024
DEFINITION  lacI:LVA device (constitutive promoter, RBS and terminator)
ACCESSION   BBa_K1088020
VERSION     BBa_K1088020.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23106
     RBS             36..50
                     /label=RBS-1\Strong
     RBS             43..47
                     /label=RBS
     misc_feature    36..50
                     /label=BBa_B0030
     RBS             36..50
                     /label=BBa_B0030
     misc_feature    51..56
                     /label=BBa_K1088022
     CDS             57..1184
                     /label=LacI-LVA
     misc_feature    1146..1184
                     /label=LVA
     CDS             57..1184
                     /label=BBa_K1088023
     misc_feature    1185..1218
                     /label=B1002
     stem_loop       1194..1209
                     /label=stem loop
     polyA_site      1209..1215
                     /label=Poly A tail
     polyA_site      1188..1193
                     /label=Poly A tail
     terminator      1185..1218
                     /label=BBa_B1002
ORIGIN
        1 tttacggcta gctcagtcct aggtatagtg ctagcattaa agaggagaaa tactagatgg
       61 tgaatgtgaa accagtaacg ttatacgatg tcgcagagta tgccggtgtc tcttatcaga
      121 ccgtttcccg cgtggtgaac caggccagcc acgtttctgc gaaaacgcgg gaaaaagtgg
      181 aagcggcgat ggcggagctg aattacattc ccaaccgcgt ggcacaacaa ctggcgggca
      241 aacagtcgtt gctgattggc gttgccacct ccagtctggc cctgcacgcg ccgtcgcaaa
      301 ttgtcgcggc gattaaatct cgcgccgatc aactgggtgc cagcgtggtg gtgtcgatgg
      361 tagaacgaag cggcgtcgaa gcctgtaaag cggcggtgca caatcttctc gcgcaacgcg
      421 tcagtgggct gatcattaac tatccgctgg atgaccagga tgccattgct gtggaagctg
      481 cctgcactaa tgttccggcg ttatttcttg atgtctctga ccagacaccc atcaacagta
      541 ttattttctc ccatgaagac ggtacgcgac tgggcgtgga gcatctggtc gcattgggtc
      601 accagcaaat cgcgctgtta gcgggcccat taagttctgt ctcggcgcgt ctgcgtctgg
      661 ctggctggca taaatatctc actcgcaatc aaattcagcc gatagcggaa cgggaaggcg
      721 actggagtgc catgtccggt tttcaacaaa ccatgcaaat gctgaatgag ggcatcgttc
      781 ccactgcgat gctggttgcc aacgatcaga tggcgctggg cgcaatgcgc gccattaccg
      841 agtccgggct gcgcgttggt gcggatatct cggtagtggg atacgacgat accgaagaca
      901 gctcatgtta tatcccgccg ttaaccacca tcaaacagga ttttcgcctg ctggggcaaa
      961 ccagcgtgga ccgcttgctg caactctctc agggccaggc ggtgaagggc aatcagctgt
     1021 tgcccgtctc actggtgaaa agaaaaacca ccctggcgcc caatacgcaa accgcctctc
     1081 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     1141 ggcaggctgc aaacgacgaa aactacgctt tagtagctta ataacgcaaa aaaccccgct
     1201 tcggcggggt tttttcgc
//