| Types | DnaRegion
|
| Roles | engineered_region
Reporter
|
| Sequences | BBa_K1100006_sequence (Version 1)
|
Description
Csy4 is a member of CRISPR pathway discovered in Pseudomonas aeruginosa. It is a single endoRNase that recognizes and cleaves a 28-nucleotide repetitive sequence and produces stable transcripts with a 5???hydroxylgroup, which can eliminate unwanted interactions between 5???UTRs and translational elements such as RBSs to standardize the expression of the elements.
Notes
Csy4 is a protein used to cleavage the mRNA so that the influence from UTR will be eliminated
Source
The Csy4 part is modified from the plasmid pHMGWA-Pa14Csy4 made by Jennifer Doudna's Lab.
