LOCUS       BBa_K1104248            1886 bp    DNA     linear       17-May-2024
DEFINITION  pSufA-E0840
ACCESSION   BBa_K1104248
VERSION     BBa_K1104248.1  
FEATURES             Location/Qualifiers
     promoter        1..1000
                     /label=BBa_K362005
     RBS             1009..1023
                     /label=RBS-1\Strong
     RBS             1016..1020
                     /label=RBS
     misc_feature    1009..1023
                     /label=BBa_B0030
     RBS             1009..1023
                     /label=BBa_B0030
     CDS             1030..1749
                     /label=GFP protein
     CDS             1030..1749
                     /label=BBa_E0040
     stem_loop       1769..1812
                     /label=stem_loop
     misc_feature    1758..1837
                     /label=BBa_B0010
     terminator      1758..1837
                     /label=BBa_B0010
     stem_loop       1853..1872
                     /label=T7 TE
     misc_feature    1879..1879
                     /label=stop
     polyA_site      1873..1886
                     /label=polya
     misc_feature    1846..1886
                     /label=BBa_B0012
     terminator      1846..1886
                     /label=BBa_B0012
     misc_feature    1009..1886
                     /label=BBa_E0840
ORIGIN
        1 ctggaaatca atgctaacca cgtccgctcg gcacgagaag ggcgggtgcg cggcgtatgc
       61 aaaccgttgc atctcggttc gcgtcaccag gtctggcaga ttgaaatctt cgatgagaaa
      121 gggcgtttgt gctgttcgtc acgattgacg accgccattt tgtgacgttg tcaccagaaa
      181 agtgtgacgc atattgcttt tggtcattat ctgcacagta aacggtggta tactgtgcag
      241 gtcttgtcta aaagcgagga tgctatgtct actcaacttg atcccaccca gctggccatt
      301 gaatttttac gccgtgacca gagcaatctt tcacctgccc aatatctcaa acgtctgaaa
      361 caactagaac ttgaatttgc tgacctgctt actctctctt ctgctgaatt aaaagaagaa
      421 atctactttg cctggcgtct gggcgtgcat tgatcctgac ttaatgccgc aagttcgcca
      481 aatcgacctg gattaacctc cgtccaggtt cgatgacctt cttaatactg cttatctacc
      541 gcctgatatt gttcaaaaat aatactaatc gcagtttgtg ttaaaacggc gggttagctt
      601 tatgagatta ttcttatcgc cccttcaaga gctaagccac tgagagtgcc ggagataagc
      661 gccggatggg gtagaaaccc ttaagcctgt gtcgcacaga cttaagggtt ttcttatttc
      721 tgaacgctgc acttttttac ctatctctta aatagtcatt ttcaatacaa ctttttcttt
      781 ttcatcacag ccttaatgct ccttagataa taaccattat ctaacaatga gatacctaat
      841 tcttagcgtg cctgttaacc cacacatcag ggtctatgct tattaaaagc aggcaaaaag
      901 catgttttac ggtaaagccc ctgcgtttgc tgggttgaac tgataatcat tatcactaac
      961 atgctgttat acgctgaaag cgatgaagtg aggtaaatcg tactagagat taaagaggag
     1021 aaatactaga tgcgtaaagg agaagaactt ttcactggag ttgtcccaat tcttgttgaa
     1081 ttagatggtg atgttaatgg gcacaaattt tctgtcagtg gagagggtga aggtgatgca
     1141 acatacggaa aacttaccct taaatttatt tgcactactg gaaaactacc tgttccatgg
     1201 ccaacacttg tcactacttt cggttatggt gttcaatgct ttgcgagata cccagatcat
     1261 atgaaacagc atgacttttt caagagtgcc atgcccgaag gttatgtaca ggaaagaact
     1321 atatttttca aagatgacgg gaactacaag acacgtgctg aagtcaagtt tgaaggtgat
     1381 acccttgtta atagaatcga gttaaaaggt attgatttta aagaagatgg aaacattctt
     1441 ggacacaaat tggaatacaa ctataactca cacaatgtat acatcatggc agacaaacaa
     1501 aagaatggaa tcaaagttaa cttcaaaatt agacacaaca ttgaagatgg aagcgttcaa
     1561 ctagcagacc attatcaaca aaatactcca attggcgatg gccctgtcct tttaccagac
     1621 aaccattacc tgtccacaca atctgccctt tcgaaagatc ccaacgaaaa gagagaccac
     1681 atggtccttc ttgagtttgt aacagctgct gggattacac atggcatgga tgaactatac
     1741 aaataataat actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc
     1801 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc
     1861 ttcgggtggg cctttctgcg tttata
//