LOCUS BBa_K1104702 7343 bp DNA linear 28-May-2024 DEFINITION Positive feedback produced by T7 system ACCESSION BBa_K1104702 VERSION BBa_K1104702.1 FEATURES Location/Qualifiers promoter 1..46 /label=BBa_I712074 misc_feature 59..62 /label=conserved RBS 55..66 /label=BBa_B0034 misc_feature 73..75 /label=start misc_feature 2722..2724 /label=stop misc_feature 2725..2727 /label=stop CDS 76..2721 /label=protein CDS 73..2727 /label=BBa_K145001 misc_feature 2740..2743 /label=conserved RBS 2736..2747 /label=BBa_B0034 CDS 2754..3428 /label=mrfp1 misc_feature 3435..3459 /label=Help:Barcodes CDS 2754..3434 /label=BBa_E1010 stem_loop 3479..3522 /label=stem_loop misc_feature 3468..3547 /label=BBa_B0010 terminator 3468..3547 /label=BBa_B0010 stem_loop 3563..3582 /label=T7 TE misc_feature 3589..3589 /label=stop polyA_site 3583..3596 /label=polya misc_feature 3556..3596 /label=BBa_B0012 terminator 3556..3596 /label=BBa_B0012 misc_feature 2736..3596 /label=BBa_I13507 promoter 3605..3639 /label=BBa_J23102 RBS 3648..3662 /label=RBS-1\Strong RBS 3655..3659 /label=RBS misc_feature 3648..3662 /label=BBa_B0030 RBS 3648..3662 /label=BBa_B0030 CDS 3669..4388 /label=GFP protein CDS 3669..4388 /label=BBa_E0040 stem_loop 4408..4451 /label=stem_loop misc_feature 4397..4476 /label=BBa_B0010 terminator 4397..4476 /label=BBa_B0010 stem_loop 4492..4511 /label=T7 TE misc_feature 4518..4518 /label=stop polyA_site 4512..4525 /label=polya misc_feature 4485..4525 /label=BBa_B0012 terminator 4485..4525 /label=BBa_B0012 misc_feature 3648..4525 /label=BBa_E0840 misc_feature 4538..4541 /label=conserved RBS 4534..4545 /label=BBa_B0034 misc_feature 4552..4554 /label=start misc_feature 7201..7203 /label=stop misc_feature 7204..7206 /label=stop CDS 4555..7200 /label=protein CDS 4552..7206 /label=BBa_K145001 stem_loop 7226..7269 /label=stem_loop misc_feature 7215..7294 /label=BBa_B0010 terminator 7215..7294 /label=BBa_B0010 stem_loop 7310..7329 /label=T7 TE misc_feature 7336..7336 /label=stop polyA_site 7330..7343 /label=polya misc_feature 7303..7343 /label=BBa_B0012 terminator 7303..7343 /label=BBa_B0012 terminator 7215..7343 /label=BBa_B0015 ORIGIN 1 taatacgact cactataggg aatacaagct acttgttctt tttgcatact agagaaagag 61 gagaaatact agatgaacac gattaacatc gctaagaacg acttctctga catcgaactg 121 gctgctatcc cgttcaacac tctggctgac cattacggtg agcgtttagc tcgcgaacag 181 ttggcccttg agcatgagtc ttacgagatg ggtgaagcac gcttccgcaa gatgtttgag 241 cgtcaactta aagctggtga ggttgcggat aacgctgccg ccaagcctct catcactacc 301 ctactcccta agatgattgc acgcatcaac gactggtttg aggaagtgaa agctaagcgc 361 ggcaagcgcc cgacagcctt ccagttcctg caagaaatca agccggaagc cgtagcgtac 421 atcaccatta agaccactct ggcttgccta accagtgctg acaatacaac cgttcaggct 481 gtagcaagcg caatcggtcg ggccattgag gacgaggctc gcttcggtcg tatccgtgac 541 cttgaagcta agcacttcaa gaaaaacgtt gaggaacaac tcaacaagcg cgtagggcac 601 gtctacaaga aagcatttat gcaagttgtc gaggctgaca tgctctctaa gggtctactc 661 ggtggcgagg cgtggtcttc gtggcataag gaagactcta ttcatgtagg agtacgctgc 721 atcgagatgc tcattgagtc aaccggaatg gttagcttac accgccaaaa tgctggcgta 781 gtaggtcaag actctgagac tatcgaactc gcacctgaat acgctgaggc tatcgcaacc 841 cgtgcaggtg cgctggctgg catctctccg atgttccaac cttgcgtagt tcctcctaag 901 ccgtggactg gcattactgg tggtggctat tgggctaacg gtcgtcgtcc tctggcgctg 961 gtgcgtactc acagtaagaa agcactgatg cgctacgaag acgtttacat gcctgaggtg 1021 tacaaagcga ttaacattgc gcaaaacacc gcatggaaaa tcaacaagaa agtcctagcg 1081 gtcgccaacg taatcaccaa gtggaagcat tgtccggtcg aggacatccc tgcgattgag 1141 cgtgaagaac tcccgatgaa accggaagac atcgacatga atcctgaggc tctcaccgcg 1201 tggaaacgtg ctgccgctgc tgtgtaccgc aaggacaagg ctcgcaagtc tcgccgtatc 1261 agccttgagt tcatgcttga gcaagccaat aagtttgcta accataaggc catctggttc 1321 ccttacaaca tggactggcg cggtcgtgtt tacgctgtgt caatgttcaa cccgcaaggt 1381 aacgatatga ccaaaggact gcttacgctg gcgaaaggta aaccaatcgg taaggaaggt 1441 tactactggc tgaaaatcca cggtgcaaac tgtgcgggtg tcgataaggt tccgttccct 1501 gagcgcatca agttcattga ggaaaaccac gagaacatca tggcttgcgc taagtctcca 1561 ctggagaaca cttggtgggc tgagcaagat tctccgttct gcttccttgc gttctgcttt 1621 gagtacgctg gggtacagca ccacggcctg agctataact gctcccttcc gctggcgttt 1681 gacgggtctt gctctggcat ccagcacttc tccgcgatgc tccgagatga ggtaggtggt 1741 cgcgcggtta acttgcttcc tagtgaaacc gttcaggaca tctacgggat tgttgctaag 1801 aaagtcaacg agattctaca agcagacgca atcaatggga ccgataacga agtagttacc 1861 gtgaccgatg agaacactgg tgaaatctct gagaaagtca agctgggcac taaggcactg 1921 gctggtcaat ggctggctta cggtgttact cgcagtgtga ctaagcgttc agtcatgacg 1981 ctggcttacg ggtccaaaga gttcggcttc cgtcaacaag tgctggaaga taccattcag 2041 ccagctattg attccggcaa gggtctgatg ttcactcagc cgaatcaggc tgctggatac 2101 atggctaagc tgatttggga atctgtgagc gtgacggtgg tagctgcggt tgaagcaatg 2161 aactggctta agtctgctgc taagctgctg gctgctgagg tcaaagataa gaagactgga 2221 gagattcttc gcaagcgttg cgctgtgcat tgggtaactc ctgatggttt ccctgtgtgg 2281 caggaataca agaagcctat tcagacgcgc ttgaacctga tgttcctcgg tcagttccgc 2341 ttacagccta ccattaacac caacaaagat agcgagattg atgcacacaa acaggagtct 2401 ggtatcgctc ctaactttgt acacagccaa gacggtagcc accttcgtaa gactgtagtg 2461 tgggcacacg agaagtacgg aatcgaatct tttgcactga ttcacgactc cttcggtacc 2521 attccggctg acgctgcgaa cctgttcaaa gcagtgcgcg aaactatggt tgacacatat 2581 gagtcttgtg atgtactggc tgatttctac gaccagttcg ctgaccagtt gcacgagtct 2641 caattggaca aaatgccagc acttccggct aaaggtaact tgaacctccg tgacatctta 2701 gagtcggact tcgcgttcgc gtaataatac tagagaaaga ggagaaatac tagatggctt 2761 cctccgaaga cgttatcaaa gagttcatgc gtttcaaagt tcgtatggaa ggttccgtta 2821 acggtcacga gttcgaaatc gaaggtgaag gtgaaggtcg tccgtacgaa ggtacccaga 2881 ccgctaaact gaaagttacc aaaggtggtc cgctgccgtt cgcttgggac atcctgtccc 2941 cgcagttcca gtacggttcc aaagcttacg ttaaacaccc ggctgacatc ccggactacc 3001 tgaaactgtc cttcccggaa ggtttcaaat gggaacgtgt tatgaacttc gaagacggtg 3061 gtgttgttac cgttacccag gactcctccc tgcaagacgg tgagttcatc tacaaagtta 3121 aactgcgtgg taccaacttc ccgtccgacg gtccggttat gcagaaaaaa accatgggtt 3181 gggaagcttc caccgaacgt atgtacccgg aagacggtgc tctgaaaggt gaaatcaaaa 3241 tgcgtctgaa actgaaagac ggtggtcact acgacgctga agttaaaacc acctacatgg 3301 ctaaaaaacc ggttcagctg ccgggtgctt acaaaaccga catcaaactg gacatcacct 3361 cccacaacga agactacacc atcgttgaac agtacgaacg tgctgaaggt cgtcactcca 3421 ccggtgctta ataacgctga tagtgctagt gtagatcgct actagagcca ggcatcaaat 3481 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa 3541 cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttatatact 3601 agagttgaca gctagctcag tcctaggtac tgtgctagct actagagatt aaagaggaga 3661 aatactagat gcgtaaagga gaagaacttt tcactggagt tgtcccaatt cttgttgaat 3721 tagatggtga tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa 3781 catacggaaa acttaccctt aaatttattt gcactactgg aaaactacct gttccatggc 3841 caacacttgt cactactttc ggttatggtg ttcaatgctt tgcgagatac ccagatcata 3901 tgaaacagca tgactttttc aagagtgcca tgcccgaagg ttatgtacag gaaagaacta 3961 tatttttcaa agatgacggg aactacaaga cacgtgctga agtcaagttt gaaggtgata 4021 cccttgttaa tagaatcgag ttaaaaggta ttgattttaa agaagatgga aacattcttg 4081 gacacaaatt ggaatacaac tataactcac acaatgtata catcatggca gacaaacaaa 4141 agaatggaat caaagttaac ttcaaaatta gacacaacat tgaagatgga agcgttcaac 4201 tagcagacca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt ttaccagaca 4261 accattacct gtccacacaa tctgcccttt cgaaagatcc caacgaaaag agagaccaca 4321 tggtccttct tgagtttgta acagctgctg ggattacaca tggcatggat gaactataca 4381 aataataata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc 4441 tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct 4501 tcgggtgggc ctttctgcgt ttatatacta gagaaagagg agaaatacta gatgaacacg 4561 attaacatcg ctaagaacga cttctctgac atcgaactgg ctgctatccc gttcaacact 4621 ctggctgacc attacggtga gcgtttagct cgcgaacagt tggcccttga gcatgagtct 4681 tacgagatgg gtgaagcacg cttccgcaag atgtttgagc gtcaacttaa agctggtgag 4741 gttgcggata acgctgccgc caagcctctc atcactaccc tactccctaa gatgattgca 4801 cgcatcaacg actggtttga ggaagtgaaa gctaagcgcg gcaagcgccc gacagccttc 4861 cagttcctgc aagaaatcaa gccggaagcc gtagcgtaca tcaccattaa gaccactctg 4921 gcttgcctaa ccagtgctga caatacaacc gttcaggctg tagcaagcgc aatcggtcgg 4981 gccattgagg acgaggctcg cttcggtcgt atccgtgacc ttgaagctaa gcacttcaag 5041 aaaaacgttg aggaacaact caacaagcgc gtagggcacg tctacaagaa agcatttatg 5101 caagttgtcg aggctgacat gctctctaag ggtctactcg gtggcgaggc gtggtcttcg 5161 tggcataagg aagactctat tcatgtagga gtacgctgca tcgagatgct cattgagtca 5221 accggaatgg ttagcttaca ccgccaaaat gctggcgtag taggtcaaga ctctgagact 5281 atcgaactcg cacctgaata cgctgaggct atcgcaaccc gtgcaggtgc gctggctggc 5341 atctctccga tgttccaacc ttgcgtagtt cctcctaagc cgtggactgg cattactggt 5401 ggtggctatt gggctaacgg tcgtcgtcct ctggcgctgg tgcgtactca cagtaagaaa 5461 gcactgatgc gctacgaaga cgtttacatg cctgaggtgt acaaagcgat taacattgcg 5521 caaaacaccg catggaaaat caacaagaaa gtcctagcgg tcgccaacgt aatcaccaag 5581 tggaagcatt gtccggtcga ggacatccct gcgattgagc gtgaagaact cccgatgaaa 5641 ccggaagaca tcgacatgaa tcctgaggct ctcaccgcgt ggaaacgtgc tgccgctgct 5701 gtgtaccgca aggacaaggc tcgcaagtct cgccgtatca gccttgagtt catgcttgag 5761 caagccaata agtttgctaa ccataaggcc atctggttcc cttacaacat ggactggcgc 5821 ggtcgtgttt acgctgtgtc aatgttcaac ccgcaaggta acgatatgac caaaggactg 5881 cttacgctgg cgaaaggtaa accaatcggt aaggaaggtt actactggct gaaaatccac 5941 ggtgcaaact gtgcgggtgt cgataaggtt ccgttccctg agcgcatcaa gttcattgag 6001 gaaaaccacg agaacatcat ggcttgcgct aagtctccac tggagaacac ttggtgggct 6061 gagcaagatt ctccgttctg cttccttgcg ttctgctttg agtacgctgg ggtacagcac 6121 cacggcctga gctataactg ctcccttccg ctggcgtttg acgggtcttg ctctggcatc 6181 cagcacttct ccgcgatgct ccgagatgag gtaggtggtc gcgcggttaa cttgcttcct 6241 agtgaaaccg ttcaggacat ctacgggatt gttgctaaga aagtcaacga gattctacaa 6301 gcagacgcaa tcaatgggac cgataacgaa gtagttaccg tgaccgatga gaacactggt 6361 gaaatctctg agaaagtcaa gctgggcact aaggcactgg ctggtcaatg gctggcttac 6421 ggtgttactc gcagtgtgac taagcgttca gtcatgacgc tggcttacgg gtccaaagag 6481 ttcggcttcc gtcaacaagt gctggaagat accattcagc cagctattga ttccggcaag 6541 ggtctgatgt tcactcagcc gaatcaggct gctggataca tggctaagct gatttgggaa 6601 tctgtgagcg tgacggtggt agctgcggtt gaagcaatga actggcttaa gtctgctgct 6661 aagctgctgg ctgctgaggt caaagataag aagactggag agattcttcg caagcgttgc 6721 gctgtgcatt gggtaactcc tgatggtttc cctgtgtggc aggaatacaa gaagcctatt 6781 cagacgcgct tgaacctgat gttcctcggt cagttccgct tacagcctac cattaacacc 6841 aacaaagata gcgagattga tgcacacaaa caggagtctg gtatcgctcc taactttgta 6901 cacagccaag acggtagcca ccttcgtaag actgtagtgt gggcacacga gaagtacgga 6961 atcgaatctt ttgcactgat tcacgactcc ttcggtacca ttccggctga cgctgcgaac 7021 ctgttcaaag cagtgcgcga aactatggtt gacacatatg agtcttgtga tgtactggct 7081 gatttctacg accagttcgc tgaccagttg cacgagtctc aattggacaa aatgccagca 7141 cttccggcta aaggtaactt gaacctccgt gacatcttag agtcggactt cgcgttcgcg 7201 taataatact agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt 7261 tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc 7321 gggtgggcct ttctgcgttt ata //