LOCUS       BBa_K1104901            2777 bp    DNA     linear       20-May-2024
DEFINITION  ROS sensor and LuxI, LuxR, TetR generator
ACCESSION   BBa_K1104901
VERSION     BBa_K1104901.1  
FEATURES             Location/Qualifiers
     misc_feature    118..137
                     /label=OxyR binding site1
     misc_feature    141..157
                     /label=OxyR binding site2
     promoter        166..171
                     /label=(AhpCp2)-35
     promoter        192..197
                     /label=(AhpCp2)-10
     misc_feature    233..238
                     /label=ctgcag->ctacag
     misc_feature    235..235
                     /label=g->a
     promoter        285..309
                     /label=DsbGp
     misc_feature    356..372
                     /label=OxyR binding site3
     misc_feature    378..394
                     /label=OxyR binding site4
     promoter        393..398
                     /label=(AhpCp1)-35
     promoter        417..422
                     /label=(AhpCp1)-10
     promoter        1..453
                     /label=BBa_K1104204
     misc_feature    466..469
                     /label=conserved
     RBS             462..473
                     /label=BBa_B0034
     misc_feature    1059..1090
                     /label=LVA
     CDS             480..1058
                     /label=luxI
     misc_feature    480..1097
                     /label=BBa_C0061
     misc_feature    1098..1122
                     /label=Help:Barcodes
     CDS             480..1097
                     /label=BBa_C0061
     misc_feature    1135..1138
                     /label=conserved
     RBS             1131..1142
                     /label=BBa_B0034
     misc_feature    1149..1150
                     /label=prefix
     misc_feature    1322..1322
                     /label=T
     misc_feature    1640..1640
                     /label=A
     CDS             1149..1898
                     /label=luxR
     misc_feature    1149..1904
                     /label=BBa_C0062
     misc_feature    1905..1929
                     /label=Help:Barcodes
     CDS             1149..1904
                     /label=BBa_C0062
     misc_feature    462..1929
                     /label=BBa_K1104911
     misc_feature    1942..1945
                     /label=conserved
     RBS             1938..1949
                     /label=BBa_B0034
     CDS             1959..2575
                     /label=tetR
     misc_feature    2576..2609
                     /label=SsrA
     misc_feature    2616..2640
                     /label=Help:Barcodes
     CDS             1956..2615
                     /label=BBa_C0040
     stem_loop       2660..2703
                     /label=stem_loop
     misc_feature    2649..2728
                     /label=BBa_B0010
     terminator      2649..2728
                     /label=BBa_B0010
     stem_loop       2744..2763
                     /label=T7 TE
     misc_feature    2770..2770
                     /label=stop
     polyA_site      2764..2777
                     /label=polya
     misc_feature    2737..2777
                     /label=BBa_B0012
     terminator      2737..2777
                     /label=BBa_B0012
     misc_feature    1938..2777
                     /label=BBa_P0440
ORIGIN
        1 caatcgcttt tactggagca ggaagttcct ctgcgaaggc gattgcagga agcagagcca
       61 gtaaaagtat cttttttaac attaatttgt ccttttcagt cagtgcaaaa gtcgagtaaa
      121 aggcataacc tatcactgtc ataggtaaga gcttagatca ggtgattgcc ctttgtttat
      181 gagggtgttg taatccatgt cgttgttgca tttgtaaggg caacacctca gcctacaggc
      241 aggcactgaa gataccaaag ggtagttcag attacacggt cacctggaaa gggggccatt
      301 ttacttttta tcgccgctgg cggtgcaaag ttcacaaagt tgtcttacga aggttgtaag
      361 gtaaaactta tcgatttgat aatggaaacg cattagccga atcggcaaaa attggttacc
      421 ttacatctca tcgaaaacac ggaggaagta tagtactaga gaaagaggag aaatactaga
      481 tgactataat gataaaaaaa tcggattttt tggcaattcc atcggaggag tataaaggta
      541 ttctaagtct tcgttatcaa gtgtttaagc aaagacttga gtgggactta gttgtagaaa
      601 ataaccttga atcagatgag tatgataact caaatgcaga atatatttat gcttgtgatg
      661 atactgaaaa tgtaagtgga tgctggcgtt tattacctac aacaggtgat tatatgctga
      721 aaagtgtttt tcctgaattg cttggtcaac agagtgctcc caaagatcct aatatagtcg
      781 aattaagtcg ttttgctgta ggtaaaaata gctcaaagat aaataactct gctagtgaaa
      841 ttacaatgaa actatttgaa gctatatata aacacgctgt tagtcaaggt attacagaat
      901 atgtaacagt aacatcaaca gcaatagagc gatttttaaa gcgtattaaa gttccttgtc
      961 atcgtattgg agacaaagaa attcatgtat taggtgatac taaatcggtt gtattgtcta
     1021 tgcctattaa tgaacagttt aaaaaagcag tcttaaatgc tgcaaacgac gaaaactacg
     1081 ctttagtagc ttaataactc tgatagtgct agtgtagatc tctactagag aaagaggaga
     1141 aatactagat gaaaaacata aatgccgacg acacatacag aataattaat aaaattaaag
     1201 cttgtagaag caataatgat attaatcaat gcttatctga tatgactaaa atggtacatt
     1261 gtgaatatta tttactcgcg atcatttatc ctcattctat ggttaaatct gatatttcaa
     1321 tcctagataa ttaccctaaa aaatggaggc aatattatga tgacgctaat ttaataaaat
     1381 atgatcctat agtagattat tctaactcca atcattcacc aattaattgg aatatatttg
     1441 aaaacaatgc tgtaaataaa aaatctccaa atgtaattaa agaagcgaaa acatcaggtc
     1501 ttatcactgg gtttagtttc cctattcata cggctaacaa tggcttcgga atgcttagtt
     1561 ttgcacattc agaaaaagac aactatatag atagtttatt tttacatgcg tgtatgaaca
     1621 taccattaat tgttccttct ctagttgata attatcgaaa aataaatata gcaaataata
     1681 aatcaaacaa cgatttaacc aaaagagaaa aagaatgttt agcgtgggca tgcgaaggaa
     1741 aaagctcttg ggatatttca aaaatattag gttgcagtga gcgtactgtc actttccatt
     1801 taaccaatgc gcaaatgaaa ctcaatacaa caaaccgctg ccaaagtatt tctaaagcaa
     1861 ttttaacagg agcaattgat tgcccatact ttaaaaatta ataacactga tagtgctagt
     1921 gtagatcact actagagaaa gaggagaaat actagatgtc cagattagat aaaagtaaag
     1981 tgattaacag cgcattagag ctgcttaatg aggtcggaat cgaaggttta acaacccgta
     2041 aactcgccca gaagctaggt gtagagcagc ctacattgta ttggcatgta aaaaataagc
     2101 gggctttgct cgacgcctta gccattgaga tgttagatag gcaccatact cacttttgcc
     2161 ctttagaagg ggaaagctgg caagattttt tacgtaataa cgctaaaagt tttagatgtg
     2221 ctttactaag tcatcgcgat ggagcaaaag tacatttagg tacacggcct acagaaaaac
     2281 agtatgaaac tctcgaaaat caattagcct ttttatgcca acaaggtttt tcactagaga
     2341 atgcattata tgcactcagc gctgtggggc attttacttt aggttgcgta ttggaagatc
     2401 aagagcatca agtcgctaaa gaagaaaggg aaacacctac tactgatagt atgccgccat
     2461 tattacgaca agctatcgaa ttatttgatc accaaggtgc agagccagcc ttcttattcg
     2521 gccttgaatt gatcatatgc ggattagaaa aacaacttaa atgtgaaagt gggtccgctg
     2581 caaacgacga aaactacgct ttagtagctt aataacactg atagtgctag tgtagatcac
     2641 tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt
     2701 tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg
     2761 gcctttctgc gtttata
//