LOCUS       BBa_K1105000            2206 bp    DNA     linear       17-May-2024
DEFINITION  lacIq-T7-ccdB
ACCESSION   BBa_K1105000
VERSION     BBa_K1105000.1  
FEATURES             Location/Qualifiers
     misc_feature    44..49
                     /label=-35box
     misc_feature    67..72
                     /label=-10box
     CDS             108..230
                     /label=LacI
     CDS             231..1190
                     /label=LacIq
     promoter        1526..1542
                     /label=pT7
     misc_feature    1547..1567
                     /label=LacO
     RBS             1600..1605
                     /label=RBS-1
     CDS             1613..1921
                     /label=ccdB
     misc_feature    1613..1615
                     /label=ccdB Start codon
     misc_feature    1919..1921
                     /label=ccdB Stop codon
ORIGIN
        1 tgcgcctgat catgttcgtc atttacgttg acaccatcga atggtgcaaa acctttcgcg
       61 gtatggcatg atagcgcccg gaagagagtc aattcagggt ggtgaatgtg aaaccagtaa
      121 cgttatacga tgtcgcagag tatgccggtg tctcttatca gaccgtttcc cgcgtggtga
      181 accaggccag ccacgtttct gcgaaaacgc gggaaaaagt ggaagcggcg atggcggagc
      241 tgaattacat tcccaaccgc gtggcacaac aactggcggg caaacagtcg ttgctgattg
      301 gcgttgccac ctccagtctg gccctgcacg cgccgtcgca aattgtcgcg gcgattaaat
      361 ctcgcgccga tcaactgggt gccagcgtgg tggtgtcgat ggtagaacga agcggcgtcg
      421 aagcctgtaa agcggcggtg cacaatcttc tcgcgcaacg cgtcagtggg ctgatcatta
      481 actatccgct ggatgaccag gatgccattg ctgtggaagc tgcctgcact aatgttccgg
      541 cgttatttct tgatgtctct gaccagacac ccatcaacag tattattttc tcccatgaag
      601 acggtacgcg actgggcgtg gagcatctgg tcgcattggg tcaccagcaa atcgcgctgt
      661 tagcgggccc attaagttct gtctcggcgc gtctgcgtct ggctggctgg cataaatatc
      721 tcactcgcaa tcaaattcag ccgatagcgg aacgggaagg cgactggagt gccatgtccg
      781 gttttcaaca aaccatgcaa atgctgaatg agggcatcgt tcccactgcg atgctggttg
      841 ccaacgatca gatggcgctg ggcgcaatgc gcgccattac cgagtccggg ctgcgcgttg
      901 gtgcggatat ctcggtagtg ggatacgacg ataccgaaga cagctcatgt tatatcccgc
      961 cgtcaaccac catcaaacag gattttcgcc tgctggggca aaccagcgtg gaccgcttgc
     1021 tgcaactctc tcagggccag gcggtgaagg gcaatcagct gttgcccgtc tcactggtga
     1081 aaagaaaaac caccctggcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
     1141 cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga gcgtgatcat
     1201 caacgcaatt aatgtgagtt agcgcgaatt gatctggttt gacagcttat catcgactgc
     1261 acggtgcacc aatgcttctg gcgtcaggca gccatcggaa gctgtggtat ggctgtgcag
     1321 gtcgtaaatc actgcataat tcgtgtcgct caaggcgcac tcccgttctg gataatgttt
     1381 tttgcgccga catcataacg gttctgggtc taggagctcg actctcttcc gggcgctatc
     1441 atgccatacc gcgaaaggtt ttgcgccatt cgatggtgtc cgggatctcg acgctctccc
     1501 ttatgcgact cctgcattag gaaattaata cgactcacta taggggaatt gtgagcggat
     1561 aacaattccc ctgtagaaat aattttgttt aactttaata aggagatata ccatggcaca
     1621 gtttaaggtt tacacctata aaagagagag ccgttatcgt ctgtttgtgg atgtacagag
     1681 tgatattatt gacacgcccg ggcgacggat ggtgatcccc ctggccagtg cacgtctgct
     1741 gtcagataaa gtctcccgtg aactttaccc ggtggtgcat atcggggatg aaagctggcg
     1801 catgatgacc accgatatgg ccagtgtgcc ggtctccgtt atcggggaag aagtggctga
     1861 tctcagccac cgcgaaaatg acatcaaaaa cgccattaac ctgatgttct ggggaatata
     1921 acctaggaga agtgaaacgc cgtagcgccg atggtagtgt ggggtctccc catgcgagag
     1981 tagggaactg ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
     2041 tttatctgtt gtttgtcggt gaacgctctc ctgagtagga caaatccgcc gggagcggat
     2101 ttgaacgttg cgaagcaacg gcccggaggg tggcgggcag gacgcccgcc ataaactgcc
     2161 aggcatcaaa ttaagcagaa ggccatcctg acggatggcc tttttc
//