BBa_K1114108
1
BBa_K1114108
Bicistronic design RBS 8, MoClo Format with BC fusion sites
2013-09-06T11:00:00Z
2015-05-08T01:09:11Z
This part was cloned off a BCD template from Sonya Iverson. Sonya Iverson based her designs for her BCD2 part off of a paper published by <a href= ???http://www.nature.com/nmeth/journal/v10/n4/full/nmeth.2404.html????>Mutalik, et al.</a>
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This is the <a href="http://2013.igem.org/Team:BostonU/MoCloChara">MoClo</a> formatted part of a bicistronic design ribosomal binding site 8 with BC fusion sites.
This is a Level 0 MoClo part with flanking sites B on the 5' side and site C on the 3' side of the part.
The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA.
Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See <a href=???http://parts.igem.org/Part:BBa_K783055???>BBa_K783055</a>.
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false
false
_1425_
0
8248
9
In stock
false
<html>
This is the <a href="http://2013.igem.org/Team:BostonU/MoCloChara">MoClo</a> formatted part of a bicistronic design ribosomal binding site 8 with BC fusion sites.
This is a Level 0 MoClo part with flanking sites B on the 5' side and site C on the 3' side of the part.
The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA.
Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See <a href=???http://parts.igem.org/Part:BBa_K783055???>BBa_K783055</a>.
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false
Traci Haddock
annotation2361725
1
MoClo Fusion Site B
range2361725
1
1
4
annotation2361726
1
MoClo Fusion Site C
range2361726
1
89
92
annotation2361723
1
BCD8
range2361723
1
5
88
BBa_K1114108_sequence
1
tactgggcccaagttcacttaaaaaggagatcaacaatgaaagcaattttcgtactgaaacatcttaatcatgcatcggaccgtttctaatg
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z