LOCUS       BBa_K1116004            3035 bp    DNA     linear       17-May-2024
DEFINITION  CAG-EYFP-miRNA-FF3
ACCESSION   BBa_K1116004
VERSION     BBa_K1116004.1  
FEATURES             Location/Qualifiers
     promoter        1..364
                     /label=CMVIE
     promoter        366..641
                     /label= Beta Actin promoter
     misc_feature    642..827
                     /label= LacO Exon
     CDS             1846..2565
                     /label=EYFP
     misc_feature    2586..2934
                     /label=miRNA FF3
ORIGIN
        1 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta
       61 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
      121 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
      181 tggactattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta
      241 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
      301 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
      361 gtcgaggtga gccccacgtt ctgcttcact ctccccatct cccccccctc cccaccccca
      421 attttgtatt tatttatttt ttaattattt tgtgcagcga tgggggcggg gggggggggg
      481 gcgcgcgcca ggcggggcgg ggcggggcga ggggcggggc ggggcgaggc ggagaggtgc
      541 ggcggcagcc aatcagagcg gcgcgctccg aaagtttcct tttatggcga ggcggcggcg
      601 gcggcggccc tataaaaagc gaagcgcgcg gcgggcggga gtcaattgtg agcggataac
      661 aattccacag tcgaccctag gttgtgtcgc gagtgttgga tcccagctga caccaattgt
      721 gagcgctcac aattgagtcg ctgcgttgcc ttcgccccgt gccccgctcc gcgccgcctc
      781 gcgccgcccg ccccggctct gactgaccgc gttactccca caggtgagcg ggcgggacgg
      841 cccttctcct ccgggctgta attagcgctt ggtttaatga cggctcgttt cttttctgtg
      901 gctgcgtgaa agccttaaag ggctccggga gggccctttg tgcggggggg agcggctcgg
      961 ggggtgcgtg cgtgtgtgtg tgcgtgggga gcgccgcgtg cggcccgcgc tgcccggcgg
     1021 ctgtgagcgc tgcgggcgcg gcgcggggct ttgtgcgctc cgcgtgtgcg cgaggggagc
     1081 gcggccgggg gcggtgcccc gcggtgcggg ggggctgcga ggggaacaaa ggctgcgtgc
     1141 ggggtgtgtg cgtggggggg tgagcagggg gtgtgggcgc ggcggtcggg ctgtaacccc
     1201 cccctgcacc cccctccccg agttgctgag cacggcccgg cttcgggtgc ggggctccgt
     1261 gcggggcgtg gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt gggggtgccg
     1321 ggcggggcgg ggccgcctcg ggccggggag ggctcggggg aggggcgcgg cggccccgga
     1381 gcgccggcgg ctgtcgaggc gcggcgagcc gcagccattg ccttttatgg taatcgtgcg
     1441 agagggcgca gggacttcct ttgtcccaaa tctggcggag ccgaaatctg ggaggcgccg
     1501 ccgcaccccc tctagcgggc gcgggcgaag cggtgcggcg ccggcaggaa ggaaatgggc
     1561 ggggagggcc ttcgtgcgtc gccgcgccgc cgtccccttc tccatctcca gcctcggggc
     1621 tgccgcaggg ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc
     1681 gtgtgaccgg cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac
     1741 agctcctggg caacgtgctg gttgttgtgc tgtctcatca ttttggcaaa gaattcgacc
     1801 caagtttgta caaaaaagca ggctgattac cggtcgccac catggtgagc aagggcgagg
     1861 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
     1921 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
     1981 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accttcggct
     2041 acggcctgca gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
     2101 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
     2161 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
     2221 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
     2281 acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca
     2341 agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
     2401 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc taccagtcca
     2461 agctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
     2521 ccgccgggat cactctcggc atggacgagc tgtacaagta agcggccgct gaggtgagta
     2581 tgtgctcgct tcggcagcac atatactatg tcgaatgagg cttcagtact ttacagaatc
     2641 gttgcctgca catcttggaa acacttgctg ggattacttc ttcaggttaa cccaacagaa
     2701 ggctcgagtg ctgttgacag tgagcgcacg atatgggctg aatacaaata gtgaagccac
     2761 agatgtattt gtattcagcc catatcgttt gcctactgcc tcggagaatt caaggggcta
     2821 ctttaggagc aattatcttg tttactaaaa ctgaatacct tgctatctct ttgatacatt
     2881 tttacaaagc tgaattaaaa tggtataaat taaatcactt ttttcaattg tttccttttt
     2941 tttcctcagg gccaagcttc gaattctgca gtcgacggta ccgcgggccc gggatccacc
     3001 ggatctagat aactgatcta cccagctttc ttgta
//