cre

BBa_K112102 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K112102

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Madhvi Venkatesh

Date created: 2008-10-20 11:00:00

Date modified: 2015-05-08 01:09:13

Cre enzyme without start or stop codons

Details

• Types: DnaRegion
• Roles: polypeptide_domain; Protein_Domain
• Sequences: BBa_K112102_sequence (Version 1)

Description

Cre enzyme without start or stop codons

Notes

This part is built in Biobrick version 2.0. In this format, parts are flanked by BglII and BamHI restriction sites.

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PCR mv001/mv004 on pBca9145-Bca1010    (1057bp, EcoRI/BamHI/DpnI)

Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)

Product is pBca1256-K112102



mv001	Forward Biobricking of 
ccataGAATTCatgAGATCTtccaatttactgaccgtacacc

mv004	Reverse Biobricking of Cre>

cgttaGGATCCatcgccatcttccagcaggcg

Source

PCR mv001/mv004 on pBca9145-Bca1010    (1057bp, EcoRI/BamHI/DpnI)

Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)

Product is pBca1256-K112102

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/myers
• synbiohub#ownedBy: user/james
• synbiohub#topLevel: BBa_K112102/1

Member of these Collections

• //function/recombination/cre
• iGEM Parts Registry