LOCUS       BBa_K112133             2215 bp    DNA     linear       22-May-2024
DEFINITION  Xis Int temperature sensitive expression cassette
ACCESSION   BBa_K112133
VERSION     BBa_K112133.1  
FEATURES             Location/Qualifiers
     promoter        1..877
                     /label=BBa_K112126
     misc_feature    878..883
                     /label=BBa_K112999
     RBS             884..899
                     /label=rbs
     CDS             903..1120
                     /label=xis
     CDS             1099..2169
                     /label=int
     misc_feature    903..905
                     /label=start
     misc_feature    2167..2169
                     /label=stop
     misc_feature    884..2169
                     /label=BBa_K112234
     misc_feature    2170..2175
                     /label=BBa_K112999
     terminator      2176..2215
                     /label=BBa_K112709
ORIGIN
        1 gaagattctt gctcaattgt tatcagctat gcgccgacca gaacaccttg ccgatcagcc
       61 aaacgtctct tcaggccact gactagcgat aactttcccc acaacggaac aactctcatt
      121 gcatgggatc attgggtact gtgggtttag tggttgtaaa aacacctgac cgctatccct
      181 gatcagtttc ttgaaggtaa actcatcacc cccaagtctg gctatgcaga aatcacctgg
      241 ctcaacagcc tgctcagggt caacgagaat taacattccg tcaggaaagc ttggcttgga
      301 gcctgttggt gcggtcatgg aattaccttc aacctcaagc cagaatgcag aatcactggc
      361 ttttttggtt gtgcttaccc atctctccgc atcacctttg gtaaaggttc taagcttagg
      421 tgagaacatc cctgcctgaa catgagaaaa aacagggtac tcatactcac ttctaagtga
      481 cggctgcata ctaaccgctt catacatctc gtagatttct ctggcgattg aagggctaaa
      541 ttcttcaacg ctaactttga gaatttttgt aagcaatgcg gcgttataag catttaatgc
      601 attgatgcca ttaaataaag caccaacgcc tgactgcccc atccccatct tgtctgcgac
      661 agattcctgg gataagccaa gttcattttt ctttttttca taaattgctt taaggcgacg
      721 tgcgtcctca agctgctctt gtgttaatgg tttctttttt gtgctcatac gttaaatcta
      781 tcaccgcaag ggataaatat ctaacaccgt gcgtgttgac tattttacct ctggcggtga
      841 taatggttgc atgtactaag gaggttgtat caattctgga tcttaattgc ggagactttg
      901 cgatgtactt gacacttcag gagtggaacg cacgccagcg acgtccaaga agccttgaaa
      961 cagttcgtcg atgggttcgg gaatgcagga tattcccacc tccggttaag gatggaagag
     1021 agtatctgtt ccacgaatca gcggtaaagg ttgacttaaa tcgaccagta acaggtggcc
     1081 ttttgaagag gatcagaaat gggaagaagg cgaagtcatg agcgccggga tttaccccct
     1141 aacctttata taagaaacaa tggatattac tgctacaggg acccaaggac gggtaaagag
     1201 tttggattag gcagagacag gcgaatcgca atcactgaag ctatacaggc caacattgag
     1261 ttattttcag gacacaaaca caagcctctg acagcgagaa tcaacagtga taattccgtt
     1321 acgttacatt catggcttga tcgctacgaa aaaatcctgg ccagcagagg aatcaagcag
     1381 aagacactca taaattacat gagcaaaatt aaagcaataa ggaggggtct gcctgatgct
     1441 ccacttgaag acatcaccac aaaagaaatt gcggcaatgc tcaatggata catagacgag
     1501 ggcaaggcgg cgtcagccaa gttaatcaga tcaacactga gcgatgcatt ccgagaggca
     1561 atagctgaag gccatataac aacaaaccat gtcgctgcca ctcgcgcagc aaaatcagag
     1621 gtaaggagat caagacttac ggctgacgaa tacctgaaaa tttatcaagc agcagaatca
     1681 tcaccatgtt ggctcagact tgcaatggaa ctggctgttg ttaccgggca acgagttggt
     1741 gatttatgcg aaatgaagtg gtctgatatc gtagatggat atctttatgt cgagcaaagc
     1801 aaaacaggcg taaaaattgc catcccaaca gcattgcata ttgatgctct cggaatatca
     1861 atgaaggaaa cacttgataa atgcaaagag attcttggcg gagaaaccat aattgcatct
     1921 actcgtcgcg aaccgctttc atccggcaca gtatcaaggt attttatgcg cgcacgaaaa
     1981 gcatcaggtc tttccttcga aggagatccg cctacctttc acgagttgcg cagtttgtct
     2041 gcaagactct atgagaagca gataagcgat aagtttgctc aacatcttct cgggcataag
     2101 tcggacacca tggcatcaca gtatcgtgat gacagaggca gggagtggga caaaattgaa
     2161 atcaaataag gatctaaaaa aaaaccccgc ccctgacagg gcggggtttt tttta
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