LOCUS BBa_K1124102 3582 bp DNA linear 13-May-2024 DEFINITION clock device (plux/tet) (+luxI, -tetR, -aiiA, --sRNA, +amilGFP)(positive feedback only) ACCESSION BBa_K1124102 VERSION BBa_K1124102.1 FEATURES Location/Qualifiers misc_feature 1..53 /label=R0062 promoter 20..25 /label=-35 promoter 42..47 /label=-10 misc_feature 54..72 /label=tetR misc_feature 53..53 /label=+1 misc_feature 1..20 /label=luxR/HSL promoter 1..72 /label=BBa_K176000 RBS 81..92 /label=B0034 CDS 99..845 /label=mCherry-LVA misc_feature 99..101 /label=Start misc_feature 810..839 /label=ssrA degradation tag: LVA misc_feature 840..842 /label=Stop misc_feature 843..845 /label=Stop misc_feature 81..845 /label=BBa_K773003 misc_feature 858..861 /label=conserved RBS 854..865 /label=BBa_B0034 misc_feature 1451..1482 /label=LVA CDS 872..1450 /label=luxI misc_feature 872..1489 /label=BBa_C0061 misc_feature 1490..1514 /label=Help:Barcodes CDS 872..1489 /label=BBa_C0061 misc_feature 854..1514 /label=BBa_C0261 stem_loop 1534..1577 /label=stem_loop misc_feature 1523..1602 /label=BBa_B0010 terminator 1523..1602 /label=BBa_B0010 stem_loop 1618..1637 /label=T7 TE misc_feature 1644..1644 /label=stop polyA_site 1638..1651 /label=polya misc_feature 1611..1651 /label=BBa_B0012 terminator 1611..1651 /label=BBa_B0012 terminator 1523..1651 /label=BBa_B0015 promoter 1660..1694 /label=BBa_J23101 misc_feature 1707..1710 /label=conserved RBS 1703..1714 /label=BBa_B0034 misc_feature 1721..1722 /label=prefix misc_feature 1894..1894 /label=T misc_feature 2212..2212 /label=A CDS 1721..2470 /label=luxR misc_feature 1721..2476 /label=BBa_C0062 misc_feature 2477..2501 /label=Help:Barcodes CDS 1721..2476 /label=BBa_C0062 stem_loop 2521..2564 /label=stem_loop misc_feature 2510..2589 /label=BBa_B0010 terminator 2510..2589 /label=BBa_B0010 stem_loop 2605..2624 /label=T7 TE misc_feature 2631..2631 /label=stop polyA_site 2625..2638 /label=polya misc_feature 2598..2638 /label=BBa_B0012 terminator 2598..2638 /label=BBa_B0012 terminator 2510..2638 /label=BBa_B0015 promoter 2647..2681 /label=BBa_J23101 misc_feature 2694..2697 /label=conserved RBS 2690..2701 /label=BBa_B0034 misc_feature 3443..3445 /label=stop misc_feature 3440..3442 /label=stop misc_feature 3407..3439 /label=LVA degradation tag RBS 2708..3406 /label=amilGFP misc_feature 2708..3445 /label=BBa_K1124000 stem_loop 3465..3508 /label=stem_loop misc_feature 3454..3533 /label=BBa_B0010 terminator 3454..3533 /label=BBa_B0010 stem_loop 3549..3568 /label=T7 TE misc_feature 3575..3575 /label=stop polyA_site 3569..3582 /label=polya misc_feature 3542..3582 /label=BBa_B0012 terminator 3542..3582 /label=BBa_B0012 terminator 3454..3582 /label=BBa_B0015 misc_feature 2690..3582 /label=BBa_K1124110 ORIGIN 1 acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga atatccctat 61 cagtgataga gatactagag aaagaggaga aatactagat ggtgagcaag ggcgaggagg 121 ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga 181 acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga 241 ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc 301 ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact 361 tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg 421 gcgtggtgac cgtgacccag gactcctcct tgcaggacgg cgagttcatc tacaaggtga 481 agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct 541 gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaagc 601 agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc acctacaagg 661 ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg gacatcacct 721 cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca 781 ccggcggcat ggacgagctg tacaaggcag caaacgacga aaactacgct ctagtagctt 841 aataatacta gagaaagagg agaaatacta gatgactata atgataaaaa aatcggattt 901 tttggcaatt ccatcggagg agtataaagg tattctaagt cttcgttatc aagtgtttaa 961 gcaaagactt gagtgggact tagttgtaga aaataacctt gaatcagatg agtatgataa 1021 ctcaaatgca gaatatattt atgcttgtga tgatactgaa aatgtaagtg gatgctggcg 1081 tttattacct acaacaggtg attatatgct gaaaagtgtt tttcctgaat tgcttggtca 1141 acagagtgct cccaaagatc ctaatatagt cgaattaagt cgttttgctg taggtaaaaa 1201 tagctcaaag ataaataact ctgctagtga aattacaatg aaactatttg aagctatata 1261 taaacacgct gttagtcaag gtattacaga atatgtaaca gtaacatcaa cagcaataga 1321 gcgattttta aagcgtatta aagttccttg tcatcgtatt ggagacaaag aaattcatgt 1381 attaggtgat actaaatcgg ttgtattgtc tatgcctatt aatgaacagt ttaaaaaagc 1441 agtcttaaat gctgcaaacg acgaaaacta cgctttagta gcttaataac tctgatagtg 1501 ctagtgtaga tctctactag agccaggcat caaataaaac gaaaggctca gtcgaaagac 1561 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc 1621 tcaccttcgg gtgggccttt ctgcgtttat atactagagt ttacagctag ctcagtccta 1681 ggtattatgc tagctactag agaaagagga gaaatactag atgaaaaaca taaatgccga 1741 cgacacatac agaataatta ataaaattaa agcttgtaga agcaataatg atattaatca 1801 atgcttatct gatatgacta aaatggtaca ttgtgaatat tatttactcg cgatcattta 1861 tcctcattct atggttaaat ctgatatttc aatcctagat aattacccta aaaaatggag 1921 gcaatattat gatgacgcta atttaataaa atatgatcct atagtagatt attctaactc 1981 caatcattca ccaattaatt ggaatatatt tgaaaacaat gctgtaaata aaaaatctcc 2041 aaatgtaatt aaagaagcga aaacatcagg tcttatcact gggtttagtt tccctattca 2101 tacggctaac aatggcttcg gaatgcttag ttttgcacat tcagaaaaag acaactatat 2161 agatagttta tttttacatg cgtgtatgaa cataccatta attgttcctt ctctagttga 2221 taattatcga aaaataaata tagcaaataa taaatcaaac aacgatttaa ccaaaagaga 2281 aaaagaatgt ttagcgtggg catgcgaagg aaaaagctct tgggatattt caaaaatatt 2341 aggttgcagt gagcgtactg tcactttcca tttaaccaat gcgcaaatga aactcaatac 2401 aacaaaccgc tgccaaagta tttctaaagc aattttaaca ggagcaattg attgcccata 2461 ctttaaaaat taataacact gatagtgcta gtgtagatca ctactagagc caggcatcaa 2521 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg 2581 aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatata 2641 ctagagttta cagctagctc agtcctaggt attatgctag ctactagaga aagaggagaa 2701 atactagatg tcttattcaa agcatggcat cgtacaagaa atgaagacga aataccatat 2761 ggaaggcagt gtcaatggcc atgaatttac gatcgaaggt gtaggaactg ggtaccctta 2821 cgaagggaaa cagatgtccg aattagtgat catcaagcct gcgggaaaac cccttccatt 2881 ctcctttgac atactgtcat cagtctttca atatggaaac cgttgcttca caaagtaccc 2941 ggcagacatg cctgactatt tcaagcaagc attcccagat ggaatgtcat atgaaaggtc 3001 atttctattt gaggatggag cagttgctac agccagctgg aacattcgtc tcgaaggaaa 3061 ttgcttcatc cacaaatcca tctttcatgg cgtaaacttt cccgctgatg gacccgtaat 3121 gaaaaagaag acaattgact gggataagtc cttcgaaaaa atgactgtgt ctaaagaggt 3181 gctaagaggt gacgtgacta tgtttcttat gctcgaagga ggtggttctc acagatgcca 3241 atttcactcc acttacaaaa cagagaagcc ggtcacactg cccccgaatc atgtcgtaga 3301 acatcaaatt gtgaggaccg accttggcca aagtgcaaaa ggctttacag tcaagctgga 3361 agcacatgcc gcggctcatg ttaacccttt gaaggttaaa aggcctgctg caaacgacga 3421 aaactacgct ttagtagctt aataatacta gagccaggca tcaaataaaa cgaaaggctc 3481 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga 3541 gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta ta //