LOCUS       BBa_K1124105            2644 bp    DNA     linear       11-Jun-2024
DEFINITION  pLac-fhlA363-plambda-sRNA(anti-hycA) (hydrogen synthesis device)
ACCESSION   BBa_K1124105
VERSION     BBa_K1124105.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     misc_feature    82..82
                     /label=start
     misc_feature    1169..1169
                     /label=E363G mutation
     misc_feature    2158..2160
                     /label=stop
     misc_feature    1987..1987
                     /label=A to G (different from E. coli K-12 MG1655)
     CDS             82..2160
                     /label=FhlA-E363G
     CDS             82..2202
                     /label=BBa_K778002
     stem_loop       2222..2265
                     /label=stem_loop
     misc_feature    2211..2290
                     /label=BBa_B0010
     terminator      2211..2290
                     /label=BBa_B0010
     stem_loop       2306..2325
                     /label=T7 TE
     misc_feature    2332..2332
                     /label=stop
     polyA_site      2326..2339
                     /label=polya
     misc_feature    2299..2339
                     /label=BBa_B0012
     terminator      2299..2339
                     /label=BBa_B0012
     terminator      2211..2339
                     /label=BBa_B0015
     promoter        2385..2390
                     /label=-10
     promoter        2362..2367
                     /label=-35
     misc_feature    2372..2388
                     /label=OR1
     misc_feature    2348..2364
                     /label=OR2
     misc_feature    2348..2396
                     /label=BBa_R0051
     promoter        2348..2396
                     /label=BBa_R0051
     misc_feature    2429..2507
                     /label=MicC scaffold
     misc_feature    2405..2428
                     /label=Target-binding sequence
     misc_feature    2405..2507
                     /label=BBa_K1124008
     stem_loop       2527..2570
                     /label=stem_loop
     misc_feature    2516..2595
                     /label=BBa_B0010
     terminator      2516..2595
                     /label=BBa_B0010
     stem_loop       2611..2630
                     /label=T7 TE
     misc_feature    2637..2637
                     /label=stop
     polyA_site      2631..2644
                     /label=polya
     misc_feature    2604..2644
                     /label=BBa_B0012
     terminator      2604..2644
                     /label=BBa_B0012
     terminator      2516..2644
                     /label=BBa_B0015
     misc_feature    2405..2644
                     /label=BBa_K1124115
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagaaagagg agaaatacta gatgtcatat acaccgatga gtgatctcgg acaacaaggg
      121 ttgttcgaca tcactcggac actattgcag cagcccgatc tggcctcgct gtgtgaggct
      181 ctttcgcaac tggtaaagcg ttctgcgctc gccgacaacg cggctattgt gttgtggcaa
      241 gcgcagactc aacgtgcgtc ttattacgcg tcgcgtgaaa aagacacccc cattaaatat
      301 gaagacgaaa ctgttctggc acacggtccg gtacgcagca ttttgtcgcg ccctgatacg
      361 ctgcattgca gttacgaaga attttgtgaa acctggccgc agctggacgc aggtgggcta
      421 tacccaaaat ttggtcacta ttgcctgatg ccactggcgg cggaagggca tatttttggt
      481 ggctgtgaat ttattcgtta tgacgatcgc ccctggagcg aaaaagagtt caatcgtctg
      541 caaacattta cgcagatcgt ttctgtcgtc accgaacaaa tccagagccg cgtcgttaac
      601 aatgtcgact atgagttgtt atgccgggaa cgcgataact tccgcatcct ggtcgccatc
      661 accaacgcgg tgctttcccg cctggatatg gacgaactgg tcagcgaagt cgccaaagaa
      721 atccattact atttcgacat tgacgatatc agtatcgtct tacgcagcca ccgtaaaaac
      781 aaactcaaca tctactccac tcactatctt gataaacagc atcccgccca cgaacagagc
      841 gaagtcgatg aagccggaac cctcaccgaa cgcgtgttca aaagtaaaga gatgctgctg
      901 atcaatctcc acgagcggga cgatttagcc ccctatgaac gcatgttgtt cgacacctgg
      961 ggcaaccaga ttcaaacctt gtgcctgtta ccgctgatgt ctggcgacac catgctgggc
     1021 gtgctgaaac tggcgcaatg cgaagagaaa gtgtttacca ctaccaatct gaatttactg
     1081 cgccagattg ccgaacgtgt ggcaatcgct gtcgataacg ccctcgccta tcaggaaatc
     1141 catcgtctga aagaacggct ggttgatgga aacctcgccc tgaccgagca gctcaacaat
     1201 gttgatagtg aatttggcga gattattggc cgcagcgaag ccatgtacag cgtgcttaaa
     1261 caagttgaaa tggtggcgca aagtgacagt accgtgctga tcctcggtga aactggcacg
     1321 ggtaaagagc tgattgcccg tgcgatccat aatctcagtg ggcgtaataa tcgccgcatg
     1381 gtcaaaatga actgcgcggc gatgcctgcc ggattgctgg aaagcgatct gtttggtcat
     1441 gagcgtgggg cttttaccgg tgccagcgcc cagcgtatcg gtcgttttga actggcggat
     1501 aaaagctccc tgttcctcga cgaagtgggc gatatgccac tggagttaca gccgaagttg
     1561 ctgcgtgtat tgcaggaaca ggagtttgaa cgtctcggca gcaacaaaat cattcagacg
     1621 gacgtgcgtc taatcgccgc gactaaccgc gatctgaaaa aaatggtcgc cgaccgtgag
     1681 ttccgtagcg atctctatta ccgcctgaac gtattcccga ttcacctgcc gccactacgc
     1741 gagcgtccgg aagatattcc gctgctggcg aaagccttta ccttcaaaat tgcccgtcgt
     1801 ctggggcgca atatcgacag cattcctgcc gagacgctgc gcaccttgag caacatggag
     1861 tggccgggta acgtacgcga actggaaaac gtcattgagc gcgcggtatt gctaacacgc
     1921 ggtaacgtgc tgcaactgtc attgccagat attgttttac cggaacctga aacgccgcct
     1981 gccgcagcgg ttgtcgccct ggagggcgaa gatgaatatc agttgattgt gcgcgtgctg
     2041 aaagaaacca acggcgtggt tgccgggcct aaaggcgctg cgcaacgtct ggggctgaaa
     2101 cgcacgaccc tgctgtcacg gatgaagcgg ctgggaattg ataaatcggc attgatttaa
     2161 ctgcaaattg ccggacagat ctgcctgtcc ggcatactat tctactagag ccaggcatca
     2221 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt
     2281 gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct gcgtttatat
     2341 actagagtaa caccgtgcgt gttgactatt ttacctctgg cggtgataat ggttgctact
     2401 agagctcgct tatttcccaa atagtcattt tctgttgggc cattgcattg ccactgattt
     2461 tccaacatat aaaaagacaa gcccgaacag tcgtccgggc ttttttttac tagagccagg
     2521 catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg
     2581 tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt
     2641 tata
//