LOCUS       BBa_K1124108            1883 bp    DNA     linear       14-Jun-2024
DEFINITION  pLambda-mCherry-pConst-c1 repressor generator (-LVA, -barcode, 
            E234K) (for UV induction check)
ACCESSION   BBa_K1124108
VERSION     BBa_K1124108.1  
FEATURES             Location/Qualifiers
     promoter        38..43
                     /label=-10
     promoter        15..20
                     /label=-35
     misc_feature    25..41
                     /label=OR1
     misc_feature    1..17
                     /label=OR2
     misc_feature    1..49
                     /label=BBa_R0051
     promoter        1..49
                     /label=BBa_R0051
     RBS             58..69
                     /label=B0034
     CDS             76..822
                     /label=mCherry-LVA
     misc_feature    76..78
                     /label=Start
     misc_feature    787..816
                     /label=ssrA degradation tag: LVA
     misc_feature    817..819
                     /label=Stop
     misc_feature    820..822
                     /label=Stop
     misc_feature    58..822
                     /label=BBa_K773003
     stem_loop       842..885
                     /label=stem_loop
     misc_feature    831..910
                     /label=BBa_B0010
     terminator      831..910
                     /label=BBa_B0010
     stem_loop       926..945
                     /label=T7 TE
     misc_feature    952..952
                     /label=stop
     polyA_site      946..959
                     /label=polya
     misc_feature    919..959
                     /label=BBa_B0012
     terminator      919..959
                     /label=BBa_B0012
     terminator      831..959
                     /label=BBa_B0015
     promoter        968..1002
                     /label=BBa_J23101
     misc_feature    1015..1018
                     /label=conserved
     RBS             1011..1022
                     /label=BBa_B0034
     misc_feature    1740..1742
                     /label=stop
     misc_feature    1743..1745
                     /label=stop
     CDS             1029..1739
                     /label=lambda cI repressor (E234K)
     misc_feature    1728..1728
                     /label=E234K (point mutation G to A)
     CDS             1029..1746
                     /label=BBa_K1124003
     stem_loop       1766..1809
                     /label=stem_loop
     misc_feature    1755..1834
                     /label=BBa_B0010
     terminator      1755..1834
                     /label=BBa_B0010
     stem_loop       1850..1869
                     /label=T7 TE
     misc_feature    1876..1876
                     /label=stop
     polyA_site      1870..1883
                     /label=polya
     misc_feature    1843..1883
                     /label=BBa_B0012
     terminator      1843..1883
                     /label=BBa_B0012
     terminator      1755..1883
                     /label=BBa_B0015
     misc_feature    1011..1883
                     /label=BBa_K1124118
ORIGIN
        1 taacaccgtg cgtgttgact attttacctc tggcggtgat aatggttgct actagagaaa
       61 gaggagaaat actagatggt gagcaagggc gaggaggata acatggccat catcaaggag
      121 ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt cgagatcgag
      181 ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag
      241 ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta cggctccaag
      301 gcctacgtga agcaccccgc cgacatcccc gactacttga agctgtcctt ccccgagggc
      361 ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt gacccaggac
      421 tcctccttgc aggacggcga gttcatctac aaggtgaagc tgcgcggcac caacttcccc
      481 tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctcctc cgagcggatg
      541 taccccgagg acggcgccct gaagggcgag atcaagcaga ggctgaagct gaaggacggc
      601 ggccactacg acgctgaggt caagaccacc tacaaggcca agaagcccgt gcagctgccc
      661 ggcgcctaca acgtcaacat caagttggac atcacctccc acaacgagga ctacaccatc
      721 gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga cgagctgtac
      781 aaggcagcaa acgacgaaaa ctacgctcta gtagcttaat aatactagag ccaggcatca
      841 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt
      901 gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct gcgtttatat
      961 actagagttt acagctagct cagtcctagg tattatgcta gctactagag aaagaggaga
     1021 aatactagat gagcacaaaa aagaaaccat taacacaaga gcagcttgag gacgcacgtc
     1081 gccttaaagc aatttatgaa aaaaagaaaa atgaacttgg cttatcccag gaatctgtcg
     1141 cagacaagat ggggatgggg cagtcaggcg ttggtgcttt atttaatggc atcaatgcat
     1201 taaatgctta taacgccgca ttgcttgcaa aaattctcaa agttagcgtt gaagaattta
     1261 gcccttcaat cgccagagaa atctacgaga tgtatgaagc ggttagtatg cagccgtcac
     1321 ttagaagtga gtatgagtac cctgtttttt ctcatgttca ggcagggatg ttctcacctg
     1381 agcttagaac ctttaccaaa ggtgatgcgg agagatgggt aagcacaacc aaaaaagcca
     1441 gtgattctgc attctggctt gaggttgaag gtaattccat gaccgcacca acaggctcca
     1501 agccaagctt tcctgacgga atgttaattc tcgttgaccc tgagcaggct gttgagccag
     1561 gtgatttctg catagccaga cttgggggtg atgagtttac cttcaagaaa ctgatcaggg
     1621 atagcggtca ggtgttttta caaccactaa acccacagta cccaatgatc ccatgcaatg
     1681 agagttgttc cgttgtgggg aaagttatcg ctagtcagtg gcctgaaaag acgtttggct
     1741 aataattact agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt
     1801 tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc
     1861 gggtgggcct ttctgcgttt ata
//