LOCUS       BBa_K1124118             873 bp    DNA     linear       01-Jun-2024
DEFINITION  cI repressor generator (-LVA, -barcode, E234K)(+RBS, +terminator+)
ACCESSION   BBa_K1124118
VERSION     BBa_K1124118.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     misc_feature    730..732
                     /label=stop
     misc_feature    733..735
                     /label=stop
     CDS             19..729
                     /label=lambda cI repressor (E234K)
     misc_feature    718..718
                     /label=E234K (point mutation G to A)
     CDS             19..736
                     /label=BBa_K1124003
     stem_loop       756..799
                     /label=stem_loop
     misc_feature    745..824
                     /label=BBa_B0010
     terminator      745..824
                     /label=BBa_B0010
     stem_loop       840..859
                     /label=T7 TE
     misc_feature    866..866
                     /label=stop
     polyA_site      860..873
                     /label=polya
     misc_feature    833..873
                     /label=BBa_B0012
     terminator      833..873
                     /label=BBa_B0012
     terminator      745..873
                     /label=BBa_B0015
ORIGIN
        1 aaagaggaga aatactagat gagcacaaaa aagaaaccat taacacaaga gcagcttgag
       61 gacgcacgtc gccttaaagc aatttatgaa aaaaagaaaa atgaacttgg cttatcccag
      121 gaatctgtcg cagacaagat ggggatgggg cagtcaggcg ttggtgcttt atttaatggc
      181 atcaatgcat taaatgctta taacgccgca ttgcttgcaa aaattctcaa agttagcgtt
      241 gaagaattta gcccttcaat cgccagagaa atctacgaga tgtatgaagc ggttagtatg
      301 cagccgtcac ttagaagtga gtatgagtac cctgtttttt ctcatgttca ggcagggatg
      361 ttctcacctg agcttagaac ctttaccaaa ggtgatgcgg agagatgggt aagcacaacc
      421 aaaaaagcca gtgattctgc attctggctt gaggttgaag gtaattccat gaccgcacca
      481 acaggctcca agccaagctt tcctgacgga atgttaattc tcgttgaccc tgagcaggct
      541 gttgagccag gtgatttctg catagccaga cttgggggtg atgagtttac cttcaagaaa
      601 ctgatcaggg atagcggtca ggtgttttta caaccactaa acccacagta cccaatgatc
      661 ccatgcaatg agagttgttc cgttgtgggg aaagttatcg ctagtcagtg gcctgaaaag
      721 acgtttggct aataattact agagccaggc atcaaataaa acgaaaggct cagtcgaaag
      781 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg
      841 gctcaccttc gggtgggcct ttctgcgttt ata
//