LOCUS       BBa_K1124119             905 bp    DNA     linear       01-Jun-2024
DEFINITION  cI repressor generator (+LVA, -barcode, E234K)(+RBS, +terminator)
ACCESSION   BBa_K1124119
VERSION     BBa_K1124119.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             19..762
                     /label=lambda cI repressor (+LVA) (E234K)
     misc_feature    730..762
                     /label=LVA degradation tag
     misc_feature    763..765
                     /label=stop
     misc_feature    766..768
                     /label=stop
     misc_feature    718..718
                     /label=E234K (point mutation G to A)
     CDS             19..768
                     /label=BBa_K1124004
     stem_loop       788..831
                     /label=stem_loop
     misc_feature    777..856
                     /label=BBa_B0010
     terminator      777..856
                     /label=BBa_B0010
     stem_loop       872..891
                     /label=T7 TE
     misc_feature    898..898
                     /label=stop
     polyA_site      892..905
                     /label=polya
     misc_feature    865..905
                     /label=BBa_B0012
     terminator      865..905
                     /label=BBa_B0012
     terminator      777..905
                     /label=BBa_B0015
ORIGIN
        1 aaagaggaga aatactagat gagcacaaaa aagaaaccat taacacaaga gcagcttgag
       61 gacgcacgtc gccttaaagc aatttatgaa aaaaagaaaa atgaacttgg cttatcccag
      121 gaatctgtcg cagacaagat ggggatgggg cagtcaggcg ttggtgcttt atttaatggc
      181 atcaatgcat taaatgctta taacgccgca ttgcttgcaa aaattctcaa agttagcgtt
      241 gaagaattta gcccttcaat cgccagagaa atctacgaga tgtatgaagc ggttagtatg
      301 cagccgtcac ttagaagtga gtatgagtac cctgtttttt ctcatgttca ggcagggatg
      361 ttctcacctg agcttagaac ctttaccaaa ggtgatgcgg agagatgggt aagcacaacc
      421 aaaaaagcca gtgattctgc attctggctt gaggttgaag gtaattccat gaccgcacca
      481 acaggctcca agccaagctt tcctgacgga atgttaattc tcgttgaccc tgagcaggct
      541 gttgagccag gtgatttctg catagccaga cttgggggtg atgagtttac cttcaagaaa
      601 ctgatcaggg atagcggtca ggtgttttta caaccactaa acccacagta cccaatgatc
      661 ccatgcaatg agagttgttc cgttgtgggg aaagttatcg ctagtcagtg gcctgaaaag
      721 acgtttggcg ctgcaaacga cgaaaactac gctttagtag cttaataata ctagagccag
      781 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
      841 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt
      901 ttata
//