LOCUS BBa_K1124200 3202 bp DNA linear 10-May-2024 DEFINITION pLux/tet-mCherry-luxI-tetR-aiiA ACCESSION BBa_K1124200 VERSION BBa_K1124200.1 FEATURES Location/Qualifiers misc_feature 1..53 /label=R0062 promoter 20..25 /label=-35 promoter 42..47 /label=-10 misc_feature 54..72 /label=tetR misc_feature 53..53 /label=+1 misc_feature 1..20 /label=luxR/HSL promoter 1..72 /label=BBa_K176000 RBS 81..92 /label=B0034 CDS 99..845 /label=mCherry-LVA misc_feature 99..101 /label=Start misc_feature 810..839 /label=ssrA degradation tag: LVA misc_feature 840..842 /label=Stop misc_feature 843..845 /label=Stop misc_feature 81..845 /label=BBa_K773003 misc_feature 858..861 /label=conserved RBS 854..865 /label=BBa_B0034 misc_feature 1451..1482 /label=LVA CDS 872..1450 /label=luxI misc_feature 872..1489 /label=BBa_C0061 misc_feature 1490..1514 /label=Help:Barcodes CDS 872..1489 /label=BBa_C0061 misc_feature 854..1514 /label=BBa_C0261 RBS 1523..1533 /label=RBS-4\Weaker RBS 1529..1532 /label=RBS misc_feature 1523..1533 /label=BBa_B0033 RBS 1523..1533 /label=BBa_B0033 CDS 1543..2159 /label=tetR misc_feature 2160..2193 /label=SsrA misc_feature 2200..2224 /label=Help:Barcodes CDS 1540..2199 /label=BBa_C0040 misc_feature 1523..2224 /label=BBa_S0107 RBS 2233..2245 /label=RBS-3\Weak RBS 2239..2242 /label=RBS misc_feature 2233..2245 /label=BBa_B0032 RBS 2233..2245 /label=BBa_B0032 misc_feature 2252..2254 /label=start misc_feature 3035..3040 /label=2 misc_feature 3002..3034 /label=LVA CDS 2252..3001 /label=aiiA misc_feature 2252..3040 /label=BBa_C0060 misc_feature 3041..3065 /label=Help:Barcodes CDS 2252..3040 /label=BBa_C0060 stem_loop 3085..3128 /label=stem_loop misc_feature 3074..3153 /label=BBa_B0010 terminator 3074..3153 /label=BBa_B0010 stem_loop 3169..3188 /label=T7 TE misc_feature 3195..3195 /label=stop polyA_site 3189..3202 /label=polya misc_feature 3162..3202 /label=BBa_B0012 terminator 3162..3202 /label=BBa_B0012 terminator 3074..3202 /label=BBa_B0015 misc_feature 2233..3202 /label=BBa_K516022 ORIGIN 1 acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga atatccctat 61 cagtgataga gatactagag aaagaggaga aatactagat ggtgagcaag ggcgaggagg 121 ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga 181 acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga 241 ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc 301 ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact 361 tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg 421 gcgtggtgac cgtgacccag gactcctcct tgcaggacgg cgagttcatc tacaaggtga 481 agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct 541 gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaagc 601 agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc acctacaagg 661 ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg gacatcacct 721 cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca 781 ccggcggcat ggacgagctg tacaaggcag caaacgacga aaactacgct ctagtagctt 841 aataatacta gagaaagagg agaaatacta gatgactata atgataaaaa aatcggattt 901 tttggcaatt ccatcggagg agtataaagg tattctaagt cttcgttatc aagtgtttaa 961 gcaaagactt gagtgggact tagttgtaga aaataacctt gaatcagatg agtatgataa 1021 ctcaaatgca gaatatattt atgcttgtga tgatactgaa aatgtaagtg gatgctggcg 1081 tttattacct acaacaggtg attatatgct gaaaagtgtt tttcctgaat tgcttggtca 1141 acagagtgct cccaaagatc ctaatatagt cgaattaagt cgttttgctg taggtaaaaa 1201 tagctcaaag ataaataact ctgctagtga aattacaatg aaactatttg aagctatata 1261 taaacacgct gttagtcaag gtattacaga atatgtaaca gtaacatcaa cagcaataga 1321 gcgattttta aagcgtatta aagttccttg tcatcgtatt ggagacaaag aaattcatgt 1381 attaggtgat actaaatcgg ttgtattgtc tatgcctatt aatgaacagt ttaaaaaagc 1441 agtcttaaat gctgcaaacg acgaaaacta cgctttagta gcttaataac tctgatagtg 1501 ctagtgtaga tctctactag agtcacacag gactactaga tgtccagatt agataaaagt 1561 aaagtgatta acagcgcatt agagctgctt aatgaggtcg gaatcgaagg tttaacaacc 1621 cgtaaactcg cccagaagct aggtgtagag cagcctacat tgtattggca tgtaaaaaat 1681 aagcgggctt tgctcgacgc cttagccatt gagatgttag ataggcacca tactcacttt 1741 tgccctttag aaggggaaag ctggcaagat tttttacgta ataacgctaa aagttttaga 1801 tgtgctttac taagtcatcg cgatggagca aaagtacatt taggtacacg gcctacagaa 1861 aaacagtatg aaactctcga aaatcaatta gcctttttat gccaacaagg tttttcacta 1921 gagaatgcat tatatgcact cagcgctgtg gggcatttta ctttaggttg cgtattggaa 1981 gatcaagagc atcaagtcgc taaagaagaa agggaaacac ctactactga tagtatgccg 2041 ccattattac gacaagctat cgaattattt gatcaccaag gtgcagagcc agccttctta 2101 ttcggccttg aattgatcat atgcggatta gaaaaacaac ttaaatgtga aagtgggtcc 2161 gctgcaaacg acgaaaacta cgctttagta gcttaataac actgatagtg ctagtgtaga 2221 tcactactag agtcacacag gaaagtacta gatgacagta aagaagcttt atttcgtccc 2281 agcaggtcgt tgtatgttgg atcattcgtc tgttaatagt acattaacac caggagaatt 2341 attagactta ccggtttggt gttatctttt ggagactgaa gaaggaccta ttttagtaga 2401 tacaggtatg ccagaaagtg cagttaataa tgaaggtctt tttaacggta catttgtcga 2461 agggcaggtt ttaccgaaaa tgactgaaga agatagaatc gtgaatattt taaaacgggt 2521 tggttatgag ccggaagacc ttctttatat tattagttct cacttgcatt ttgatcatgc 2581 aggaggaaat ggcgctttta taaatacacc aatcattgta cagcgtgctg aatatgaggc 2641 ggcgcagcat agcgaagaat atttgaaaga atgtatattg ccgaatttaa actacaaaat 2701 cattgaaggt gattatgaag tcgtaccagg agttcaatta ttgcatacac caggccatac 2761 tccagggcat caatcgctat taattgagac agaaaaatcc ggtcctgtat tattaacgat 2821 tgatgcatcg tatacgaaag agaattttga aaatgaagtg ccatttgcgg gatttgattc 2881 agaattagct ttatcttcaa ttaaacgttt aaaagaagtg gtgatgaaag agaagccgat 2941 tgttttcttt ggacatgata tagagcagga aaggggatgt aaagtgttcc ctgaatatat 3001 agctgcaaac gacgaaaact acgctttagt agcttaataa cgctgatagt gctagtgtag 3061 atcgctacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt 3121 cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg 3181 ggtgggcctt tctgcgttta ta //