LOCUS       BBa_K1124201            2361 bp    DNA     linear       09-May-2024
DEFINITION  pLux/tet-mCherry-luxI-tetR
ACCESSION   BBa_K1124201
VERSION     BBa_K1124201.1  
FEATURES             Location/Qualifiers
     misc_feature    1..53
                     /label=R0062
     promoter        20..25
                     /label=-35
     promoter        42..47
                     /label=-10
     misc_feature    54..72
                     /label=tetR
     misc_feature    53..53
                     /label=+1
     misc_feature    1..20
                     /label=luxR/HSL
     promoter        1..72
                     /label=BBa_K176000
     RBS             81..92
                     /label=B0034
     CDS             99..845
                     /label=mCherry-LVA
     misc_feature    99..101
                     /label=Start
     misc_feature    810..839
                     /label=ssrA degradation tag: LVA
     misc_feature    840..842
                     /label=Stop
     misc_feature    843..845
                     /label=Stop
     misc_feature    81..845
                     /label=BBa_K773003
     misc_feature    858..861
                     /label=conserved
     RBS             854..865
                     /label=BBa_B0034
     misc_feature    1451..1482
                     /label=LVA
     CDS             872..1450
                     /label=luxI
     misc_feature    872..1489
                     /label=BBa_C0061
     misc_feature    1490..1514
                     /label=Help:Barcodes
     CDS             872..1489
                     /label=BBa_C0061
     misc_feature    854..1514
                     /label=BBa_C0261
     RBS             1523..1533
                     /label=RBS-4\Weaker
     RBS             1529..1532
                     /label=RBS
     misc_feature    1523..1533
                     /label=BBa_B0033
     RBS             1523..1533
                     /label=BBa_B0033
     CDS             1543..2159
                     /label=tetR
     misc_feature    2160..2193
                     /label=SsrA
     misc_feature    2200..2224
                     /label=Help:Barcodes
     CDS             1540..2199
                     /label=BBa_C0040
     stem_loop       2244..2287
                     /label=stem_loop
     misc_feature    2233..2312
                     /label=BBa_B0010
     terminator      2233..2312
                     /label=BBa_B0010
     stem_loop       2328..2347
                     /label=T7 TE
     misc_feature    2354..2354
                     /label=stop
     polyA_site      2348..2361
                     /label=polya
     misc_feature    2321..2361
                     /label=BBa_B0012
     terminator      2321..2361
                     /label=BBa_B0012
     misc_feature    1523..2361
                     /label=BBa_P0340
ORIGIN
        1 acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga atatccctat
       61 cagtgataga gatactagag aaagaggaga aatactagat ggtgagcaag ggcgaggagg
      121 ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga
      181 acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga
      241 ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc
      301 ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact
      361 tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg
      421 gcgtggtgac cgtgacccag gactcctcct tgcaggacgg cgagttcatc tacaaggtga
      481 agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct
      541 gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaagc
      601 agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc acctacaagg
      661 ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg gacatcacct
      721 cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca
      781 ccggcggcat ggacgagctg tacaaggcag caaacgacga aaactacgct ctagtagctt
      841 aataatacta gagaaagagg agaaatacta gatgactata atgataaaaa aatcggattt
      901 tttggcaatt ccatcggagg agtataaagg tattctaagt cttcgttatc aagtgtttaa
      961 gcaaagactt gagtgggact tagttgtaga aaataacctt gaatcagatg agtatgataa
     1021 ctcaaatgca gaatatattt atgcttgtga tgatactgaa aatgtaagtg gatgctggcg
     1081 tttattacct acaacaggtg attatatgct gaaaagtgtt tttcctgaat tgcttggtca
     1141 acagagtgct cccaaagatc ctaatatagt cgaattaagt cgttttgctg taggtaaaaa
     1201 tagctcaaag ataaataact ctgctagtga aattacaatg aaactatttg aagctatata
     1261 taaacacgct gttagtcaag gtattacaga atatgtaaca gtaacatcaa cagcaataga
     1321 gcgattttta aagcgtatta aagttccttg tcatcgtatt ggagacaaag aaattcatgt
     1381 attaggtgat actaaatcgg ttgtattgtc tatgcctatt aatgaacagt ttaaaaaagc
     1441 agtcttaaat gctgcaaacg acgaaaacta cgctttagta gcttaataac tctgatagtg
     1501 ctagtgtaga tctctactag agtcacacag gactactaga tgtccagatt agataaaagt
     1561 aaagtgatta acagcgcatt agagctgctt aatgaggtcg gaatcgaagg tttaacaacc
     1621 cgtaaactcg cccagaagct aggtgtagag cagcctacat tgtattggca tgtaaaaaat
     1681 aagcgggctt tgctcgacgc cttagccatt gagatgttag ataggcacca tactcacttt
     1741 tgccctttag aaggggaaag ctggcaagat tttttacgta ataacgctaa aagttttaga
     1801 tgtgctttac taagtcatcg cgatggagca aaagtacatt taggtacacg gcctacagaa
     1861 aaacagtatg aaactctcga aaatcaatta gcctttttat gccaacaagg tttttcacta
     1921 gagaatgcat tatatgcact cagcgctgtg gggcatttta ctttaggttg cgtattggaa
     1981 gatcaagagc atcaagtcgc taaagaagaa agggaaacac ctactactga tagtatgccg
     2041 ccattattac gacaagctat cgaattattt gatcaccaag gtgcagagcc agccttctta
     2101 ttcggccttg aattgatcat atgcggatta gaaaaacaac ttaaatgtga aagtgggtcc
     2161 gctgcaaacg acgaaaacta cgctttagta gcttaataac actgatagtg ctagtgtaga
     2221 tcactactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc
     2281 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg
     2341 gtgggccttt ctgcgtttat a
//