LOCUS       BBa_K1124202            2492 bp    DNA     linear       14-May-2024
DEFINITION  pLux/tet-mCherry-luxI-aiiA
ACCESSION   BBa_K1124202
VERSION     BBa_K1124202.1  
FEATURES             Location/Qualifiers
     misc_feature    1..53
                     /label=R0062
     promoter        20..25
                     /label=-35
     promoter        42..47
                     /label=-10
     misc_feature    54..72
                     /label=tetR
     misc_feature    53..53
                     /label=+1
     misc_feature    1..20
                     /label=luxR/HSL
     promoter        1..72
                     /label=BBa_K176000
     RBS             81..92
                     /label=B0034
     CDS             99..845
                     /label=mCherry-LVA
     misc_feature    99..101
                     /label=Start
     misc_feature    810..839
                     /label=ssrA degradation tag: LVA
     misc_feature    840..842
                     /label=Stop
     misc_feature    843..845
                     /label=Stop
     misc_feature    81..845
                     /label=BBa_K773003
     misc_feature    858..861
                     /label=conserved
     RBS             854..865
                     /label=BBa_B0034
     misc_feature    1451..1482
                     /label=LVA
     CDS             872..1450
                     /label=luxI
     misc_feature    872..1489
                     /label=BBa_C0061
     misc_feature    1490..1514
                     /label=Help:Barcodes
     CDS             872..1489
                     /label=BBa_C0061
     misc_feature    854..1514
                     /label=BBa_C0261
     RBS             1523..1535
                     /label=RBS-3\Weak
     RBS             1529..1532
                     /label=RBS
     misc_feature    1523..1535
                     /label=BBa_B0032
     RBS             1523..1535
                     /label=BBa_B0032
     misc_feature    1542..1544
                     /label=start
     misc_feature    2325..2330
                     /label=2
     misc_feature    2292..2324
                     /label=LVA
     CDS             1542..2291
                     /label=aiiA
     misc_feature    1542..2330
                     /label=BBa_C0060
     misc_feature    2331..2355
                     /label=Help:Barcodes
     CDS             1542..2330
                     /label=BBa_C0060
     stem_loop       2375..2418
                     /label=stem_loop
     misc_feature    2364..2443
                     /label=BBa_B0010
     terminator      2364..2443
                     /label=BBa_B0010
     stem_loop       2459..2478
                     /label=T7 TE
     misc_feature    2485..2485
                     /label=stop
     polyA_site      2479..2492
                     /label=polya
     misc_feature    2452..2492
                     /label=BBa_B0012
     terminator      2452..2492
                     /label=BBa_B0012
     terminator      2364..2492
                     /label=BBa_B0015
     misc_feature    1523..2492
                     /label=BBa_K516022
ORIGIN
        1 acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga atatccctat
       61 cagtgataga gatactagag aaagaggaga aatactagat ggtgagcaag ggcgaggagg
      121 ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag ggctccgtga
      181 acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag ggcacccaga
      241 ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc
      301 ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact
      361 tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg
      421 gcgtggtgac cgtgacccag gactcctcct tgcaggacgg cgagttcatc tacaaggtga
      481 agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag accatgggct
      541 gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc gagatcaagc
      601 agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc acctacaagg
      661 ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg gacatcacct
      721 cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca
      781 ccggcggcat ggacgagctg tacaaggcag caaacgacga aaactacgct ctagtagctt
      841 aataatacta gagaaagagg agaaatacta gatgactata atgataaaaa aatcggattt
      901 tttggcaatt ccatcggagg agtataaagg tattctaagt cttcgttatc aagtgtttaa
      961 gcaaagactt gagtgggact tagttgtaga aaataacctt gaatcagatg agtatgataa
     1021 ctcaaatgca gaatatattt atgcttgtga tgatactgaa aatgtaagtg gatgctggcg
     1081 tttattacct acaacaggtg attatatgct gaaaagtgtt tttcctgaat tgcttggtca
     1141 acagagtgct cccaaagatc ctaatatagt cgaattaagt cgttttgctg taggtaaaaa
     1201 tagctcaaag ataaataact ctgctagtga aattacaatg aaactatttg aagctatata
     1261 taaacacgct gttagtcaag gtattacaga atatgtaaca gtaacatcaa cagcaataga
     1321 gcgattttta aagcgtatta aagttccttg tcatcgtatt ggagacaaag aaattcatgt
     1381 attaggtgat actaaatcgg ttgtattgtc tatgcctatt aatgaacagt ttaaaaaagc
     1441 agtcttaaat gctgcaaacg acgaaaacta cgctttagta gcttaataac tctgatagtg
     1501 ctagtgtaga tctctactag agtcacacag gaaagtacta gatgacagta aagaagcttt
     1561 atttcgtccc agcaggtcgt tgtatgttgg atcattcgtc tgttaatagt acattaacac
     1621 caggagaatt attagactta ccggtttggt gttatctttt ggagactgaa gaaggaccta
     1681 ttttagtaga tacaggtatg ccagaaagtg cagttaataa tgaaggtctt tttaacggta
     1741 catttgtcga agggcaggtt ttaccgaaaa tgactgaaga agatagaatc gtgaatattt
     1801 taaaacgggt tggttatgag ccggaagacc ttctttatat tattagttct cacttgcatt
     1861 ttgatcatgc aggaggaaat ggcgctttta taaatacacc aatcattgta cagcgtgctg
     1921 aatatgaggc ggcgcagcat agcgaagaat atttgaaaga atgtatattg ccgaatttaa
     1981 actacaaaat cattgaaggt gattatgaag tcgtaccagg agttcaatta ttgcatacac
     2041 caggccatac tccagggcat caatcgctat taattgagac agaaaaatcc ggtcctgtat
     2101 tattaacgat tgatgcatcg tatacgaaag agaattttga aaatgaagtg ccatttgcgg
     2161 gatttgattc agaattagct ttatcttcaa ttaaacgttt aaaagaagtg gtgatgaaag
     2221 agaagccgat tgttttcttt ggacatgata tagagcagga aaggggatgt aaagtgttcc
     2281 ctgaatatat agctgcaaac gacgaaaact acgctttagt agcttaataa cgctgatagt
     2341 gctagtgtag atcgctacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga
     2401 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg
     2461 ctcaccttcg ggtgggcctt tctgcgttta ta
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